UVSX Antibody

Code CSB-PA361289XA01EDZ
Size US$299
Image
  • Western Blot
    Positive WB detected in Recombinant protein
    All lanes: UVSX antibody at 1:2000
    Secondary
    Goat polyclonal to rabbit IgG at 1/50000 dilution
    Predicted band size: 23.7 kDa
    Observed band size: 28 kDa
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Product Details

Full Product Name
Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) UVSX Polyclonal antibody
Uniprot No.
Target Names
UVSX
Alternative Names
UVSXRecombination and repair protein antibody
Raised in
Rabbit
Species Reactivity
Enterobacteria phage T4 (Bacteriophage T4)
Immunogen
Recombinant Enterobacteria phage T4 (Bacteriophage T4) UVSX protein
Immunogen Species
Enterobacteria phage T4 (Bacteriophage T4)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Protein A/G
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Lead Time
Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Description

The rabbit is injected with the recombinant Enterobacteria phage T4 (Bacteriophage T4) UVSX protein to stimulate its immune system. Eventually, the rabbit's immune system produces antibodies against the UVSX protein. Once enough antibodies have been generated, a sample of serum is collected to obtain the polyclonal antibodies. The collected serum, which contains the UVSX antibody, is then subjected to protein A/G affinity chromatography. The effectiveness of the UVSX antibody is assessed in ELISA and WB applications. This specific UVSX antibody is capable of specifically reacting with the Enterobacteria phage T4 (Bacteriophage T4) UVSX protein.

The Enterobacteria phage T4 (Bacteriophage T4) UVSX protein mainly participates in the recombination and repair processes of the phage's DNA. UVSX is a recombinase enzyme that plays a crucial role in homologous recombination, allowing for the exchange of genetic material between two DNA molecules with similar sequences. This protein helps in repairing damaged DNA and facilitating the integration of the phage DNA into the host bacterium's genome. Additionally, UVSX is involved in the resolution of branched DNA structures during recombination, ensuring the successful completion of the recombination process.

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Target Background

Function
Important in genetic recombination, DNA repair, and replication. Possesses pairing and strand-transfer activity. Interacts with dda and gene 32 proteins.
Gene References into Functions
  1. the uvsX mutant executes recombination-dependent replication but does it by a qualitatively different way. PMID: 22365497
  2. The authors show that the phage helicase UvsW completes the UvsX-promoted strand-exchange reaction, allowing the generation of a simple nicked circular product rather than complex networks of partially exchanged substrates. PMID: 21035462
  3. UvsX suffice to rescue stalled T4 replication forks in vitro PMID: 15194689
  4. allosteric switch residue His195 controls the formation of an asymmetric conformation of UvsX-ssDNA filaments that is active in DNA strand exchange. PMID: 19027026
  5. unlike wild-type UvsX, the ssDNA-dependent ATPase activities of UvsX-H195Q/A are strongly inhibited by both low and high concentrations of UvsY protein. PMID: 19244311

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Protein Families
RecA family
Database Links

KEGG: vg:1258704

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