Bovine Interleukin 1,IL-1 ELISA Kit

Instructions
Code CSB-E12019B
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name Interleukin 1,IL-1
Alternative Names IL1A ELISA Kit; Interleukin-1 alpha ELISA Kit; IL-1 alpha ELISA Kit
Abbreviation IL1A
Uniprot No. P08831
Species Bos taurus (Bovine)
Sample Types serum, plasma, tissue homogenates
Detection Range 250 pg/mL-4000 pg/mL
Sensitivity 250 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<15%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %90
Range %85-94
1:2Average %92
Range %87-97
1:4Average %84
Range %80-88
1:8Average %98
Range %90-102
Recovery
The recovery of bovine IL-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9490-98
EDTA plasma (n=4)8983-96
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2Average
40000.147 0.141 0.144
20000.296 0.279 0.288
10000.431 0.467 0.449
5000.782 0.823 0.803
2501.862 1.826 1.844
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function Produced by activated macrophages, IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells.
Gene References into Functions
  1. These findings suggest that the elevated levels of IL-1alpha found in the osteoarthritis environment heighten fibroblast-like synoviocytes sensitivity to fluid shear by altering both intercellular communication and individual cell sensitivity, which could affect downstream functions and contribute to progression of the disease state PMID: 28716465
  2. Protein kinase R plays a pivotal role in oncostatin M and interleukin-1 signalling in bovine articular cartilage chondrocytes. PMID: 22287113
  3. Testicular IL-1 alpha and IL-1 beta concentrations were highest in the early post-natal period; however, IL-1 bioactivity and IL-6 concentrations were greatest in the immediate pre-pubertal period. PMID: 19144032
  4. Genes for IL-1alpha and IL-1beta are expressed and a functional IL-1R is present in bovine corpora lutea throughout luteal phase. IL-1alpha and IL-1beta may have different roles as regulating PGF(2alpha) and PGE(2) production during luteal phase. PMID: 15253926
  5. non-metalloproteinase mechanisms participate in IL-1-induced matrix degradation and loss of tissue material properties PMID: 17174540
  6. These results suggest that IL-1alpha and IL-1beta are produced by the stromal cells, that IL-1beta is produced by the epithelial cells, and that IL-1alpha is a more potent stimulator of prostaglandin and plasminogen activator in bovine endometrial cells. PMID: 18930361

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Subcellular Location Secreted
Protein Families IL-1 family
Database Links

KEGG: bta:281250

STRING: 9913.ENSBTAP00000013665

UniGene: Bt.191

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