Bovine transforming growth factors β2,TGFβ2 ELISA Kit

Code CSB-E14209B
Size 96T,5×96T,10×96T
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Product Details

Target Name
transforming growth factor, beta 2
Alternative Names
TGFB2 ELISA Kit; Transforming growth factor beta-2 proprotein ELISA Kit; Milk growth factor ELISA Kit; MGF) [Cleaved into: Latency-associated peptide ELISA Kit; LAP); Transforming growth factor beta-2 ELISA Kit; TGF-beta-2)] ELISA Kit
Abbreviation
TGFB2
Uniprot No.
Species
Bos taurus (Bovine)
Sample Types
serum, plasma, tissue homogenates
Detection Range
6.25 ng/mL-100 ng/mL
Sensitivity
6.25 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Signal Transduction
Assay Principle
quantitative
Measurement
Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<15%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<15%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of bovine TGFβ2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 82-89  
1:2 Average % 83  
Range % 80-86  
1:4 Average % 97  
Range % 93-100  
1:8 Average % 96  
Range % 91-101  
Recovery
The recovery of bovine TGFβ2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 87-93  
EDTA plasma (n=4) 94 89-99  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average    
100 0.204 0.198 0.201    
50 0.356 0.358 0.357    
25 0.592 0.572 0.582    
12.5 1.107 1.105 1.106    
6.25 2.332 2.369 2.351    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Bovine TGFB2 ELISA Kit was designed for the quantitative measurement of Bovine TGFB2 protein in serum, plasma, tissue homogenates. It is a Competitive ELISA kit, its detection range is 6.25 ng/mL-100 ng/mL and the sensitivity is 6.25 ng/mL.

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Target Background

Function
(From Uniprot)
Transforming growth factor beta-2 proprotein: Precursor of the Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains, which constitute the regulatory and active subunit of TGF-beta-2, respectively.; Required to maintain the Transforming growth factor beta-2 (TGF-beta-2) chain in a latent state during storage in extracellular matrix. Associates non-covalently with TGF-beta-2 and regulates its activation via interaction with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2.; Transforming growth factor beta-2: Multifunctional protein that regulates various processes such as angiogenesis and heart development. Activation into mature form follows different steps: following cleavage of the proprotein in the Golgi apparatus, Latency-associated peptide (LAP) and Transforming growth factor beta-2 (TGF-beta-2) chains remain non-covalently linked rendering TGF-beta-2 inactive during storage in extracellular matrix. At the same time, LAP chain interacts with 'milieu molecules', such as LTBP1 and LRRC32/GARP, that control activation of TGF-beta-2 and maintain it in a latent state during storage in extracellular milieus. Once activated following release of LAP, TGF-beta-2 acts by binding to TGF-beta receptors (TGFBR1 and TGFBR2), which transduce signal.
Gene References into Functions
  1. Data show that TGF-beta pathways operate during ovarian fetal development, and fibrillin 3 is highly expressed at a critical stage early in developing human and bovine fetal ovaries. PMID: 21411746
Subcellular Location
[Latency-associated peptide]: Secreted, extracellular space, extracellular matrix.; [Transforming growth factor beta-2]: Secreted.
Protein Families
TGF-beta family
Database Links
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