Canine N-terminal pro-brain natriuretic peptide(NT-proBNP)ELISA Kit

Code CSB-E17577c
Size 96T,5×96T,10×96T
Price Request a Quote or Start an on-line Chat
Trial Size 24T ELISA Kit Trial Size (Only USD$150/ kit)
* The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Product Details

Target Name
N-terminal pro-brain natriuretic peptide(NT-proBNP)
Abbreviation
NT-proBNP
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma
Detection Range
0.312 ng/mL-20 ng/mL
Sensitivity
0.078 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Cardiovascular
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-92
1:2Average %97
Range %90-105
1:4Average %97
Range %92-104
1:8Average %93
Range %86-100
Recovery
The recovery of canine NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9585-108
EDTA plasma (n=4)9792-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
202.675 2.655 2.665 2.516
102.152 2.144 2.148 1.999
51.311 1.302 1.307 1.158
2.50.781 0.799 0.790 0.641
1.250.377 0.363 0.370 0.221
0.6250.264 0.254 0.259 0.110
0.3120.186 0.190 0.188 0.039
00.146 0.152 0.149  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog NT-proBNP ELISA Kit was designed for the quantitative measurement of Dog NT-proBNP protein in serum, plasma. It is a Sandwich ELISA kit, its detection range is 0.312 ng/mL-20 ng/mL and the sensitivity is 0.078 ng/mL.

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 Q&A
Q:

Per customers request, please provide the following information regarding Canine N-terminal pro-brain natriuretic peptide, NT-proBNP ELISA Kit (CSB-E17577c)
Can this kit be used with cat samples too? Or is this kit only designed to be used for dogs?
1.What is the standard? (Recombinant full-length protein? Partial protein
(range?)?, peptide (sequence range?), native protein?)
2. If recombinant protein, then what is the host?
3. Is the capture antibody a monoclonal or polyclonal?
4. What is the host of the capture antibody?
5. What is the immunogen of the capture antibody?
6. Is the detection antibody a monoclonal or polyclonal?
7. What is the host of the detection antibody?
8. What is the immunogen of the detection antibody?

A:
Thanks for your inquiry!
CSB-E17577c
This kit is specific to dog samples. It is not suggested to be used to test other species.
Capture antibody is mouse monoclonal antibody and immunogen is 293 cell-derived recombinant protein(full length).
Detection antibody is goat polyclonal antibody and immunogen is 293 cell-derived recombinant protein(fragment).
Standard is 293 cell-derived recombinant protein. Sorry we can not disclose the sequence.
Pls let me know if you have any further questions. Thank you.
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