Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

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Customer Reviews and Q&A

Product Details

Citations

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Rat N-terminal pro-brain natriuretic peptide,NT-proBNP ELISA KIT

Citations (19) Protocol MSDS

Code	CSB-E08752r
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (19)
Related Products
Customer Reviews and Q&A

Target Name

N-terminal pro-brain natriuretic peptide,NT-proBNP

Abbreviation

NT-proBNP

Species

Rattus norvegicus (Rat)

Sample Types

serum, plasma, tissue homogenates

Detection Range

7.8 pg/mL-500 pg/mL

Sensitivity

1.95 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cardiovascular

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of rat NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	89
	Range %	84-95
1:2	Average %	96
	Range %	90-101
1:4	Average %	99
	Range %	94-105
1:8	Average %	84
	Range %	80-88

Recovery

The recovery of rat NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-94
EDTA plasma (n=4)	94	89-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
500	2.769	2.651	2.710	2.548
250	1.973	1.901	1.937	1.775
125	1.183	1.217	1.200	1.038
62.5	0.793	0.841	0.817	0.655
31.2	0.506	0.550	0.528	0.366
15.6	0.404	0.379	0.392	0.230
7.8	0.261	0.245	0.253	0.091
0	0.165	0.159	0.162

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Rat NT-proBNP ELISA Kit was designed for the quantitative measurement of Rat NT-proBNP protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

Shenmai injection revives cardiac function in rats with hypertensive heart failure: involvement of microbial-host co-metabolism L Li, S Zhong, J Ye, S Hu, X Xiong,BMC Complementary Medicine and Therapies,2025
Clinicopathological Studies on the Impact of Grape Seed Extract and L-Carnitine as Cardioprotective Agents Against Doxorubicin-Induced Toxicity in Rats TS Aldayel, OE Kilany, HNG El-Hak, HMA Abdelrazek,Life,2024
VPO1 Promotes Programmed Necrosis of Cardiomyocytes in Rats with Chronic Heart Failure by Upregulating CYLD Y Zhang, Z Shen, Z Mao, D Huang, C Lou,Frontiers in Bioscience-Landmark,2024
Uncovering the Therapeutic Potential of Lithium Chloride in Type 2 Diabetic Cardiomyopathy: Targeting Tau Hyperphosphorylation and TGF-β Signaling via GSK-3β Inhibition L Abou Assi,Pharmaceutics,2024
DL0805-1, a novel Rho-kinase inhibitor, attenuates lung injury and vasculopathy in a rat model of monocrotaline-induced pulmonary hypertension D Chen,European journal of pharmacology,2023
Role of Klotho and AGE/RAGE-Wnt/β-Catenin Signalling Pathway on the Development of Cardiac and Renal Fibrosis in Diabetes B Martín-Carro,International Journal of Molecular Sciences,2023
Puerarin-V Improve Mitochondrial Respiration and Cardiac Function in a Rat Model of Diabetic Cardiomyopathy via Inhibiting Pyroptosis Pathway through P2X7 Receptors S Sun,International journal of molecular sciences,2022
Early cardiac-chamber-specific fingerprints in heart failure with preserved ejection fraction detected by FTIR and Raman spectroscopic techniques N Tombolesi,Scientific Reports,2022
Renal Denervation Mitigated Fecal Microbiota Aberrations in Rats with Chronic Heart Failure Z Guo,Evidence-Based Complementary and Alternative Medicine,2021
Cardiac-chamber-specific Fingerprints in Heart Failure with Preserved Ejection Fraction. Early Detection of Pathological Conditions by FTIR and Raman Spectroscopic Techniques N Tombolesi,Research Square,2021
Xiao-Qing-Long-Tang Maintains Cardiac Function during Heart Failure with Reduced Ejection Fraction in Salt-Sensitive Rats by Regulating the Imbalance of Cardiac Sympathetic Innervation Zhaoyu Li,Evidence-Based Complementary and Alternative Medicine,2020
The cardiac protection of Baoyuan decoction via gut-heart axis metabolic pathway Z Du,Phytomedicine,2020
Chronic high-fat diet consumption induces an alteration in plasma/brain neurotensin signaling, metabolic disturbance, systemic inflammation/oxidative stress, brain … N Saiyasit,Neuropeptides,2020
Rho-Kinase inhibitors ameliorated diclofenac-induced cardiotoxicity in chloroquine-treated adjuvant arthritic rats MH Arafa,Life Sciences,2020
YQWY decoction reverses cardiac hypertrophy induced by TAC through inhibiting GATA4 phosphorylation and MAPKs Jing-JingHUANG, et al,Chinese Journal of Natural Medicines,2019
Assessment of Pregabalin-Induced Cardiotoxicity in Rats: Mechanistic Role of Angiotensin 1-7 Awwad Z M, et al,Cardiovascular Toxicology,2019
Telmisartan and Captopril Ameliorate Pregabalin-Induced Heart Failure in Rats Awwad Z M, et al,Toxicology,2019
The mechanism of RNA oxidation involved in the development of heart failure Liu T, et al,Free Radical Research,2019
Blood pressure-targeted stepwise resuscitation for hemorrhagic shock in rats. Lee JH.et al,J Trauma Acute Care Surg.,2014

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.