Pig N-terminal pro-brain natriuretic peptide (NT-proBNP) ELISA kit

Code CSB-EQ027465PI
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name N-terminal pro-brain natriuretic peptide (NT-proBNP)
Abbreviation NT-proBNP
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 7.8 pg/mL-500 pg/mL
Sensitivity 1.95 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of pig NT-proBNP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 103  
Range % 96-108  
1:2 Average % 99  
Range % 91-102  
1:4 Average % 94  
Range % 86-98  
1:8 Average % 98  
Range % 90-102  
The recovery of pig NT-proBNP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 105 99-109  
EDTA plasma (n=4) 97 90-101  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
500 2.129 2.045 2.087 1.949  
250 1.671 1.555 1.613 1.475  
125 1.134 1.117 1.126 0.988  
62.5 0.666 0.650 0.658 0.520  
31.2 0.388 0.371 0.380 0.242  
15.6 0.257 0.266 0.262 0.124  
7.8 0.171 0.167 0.169 0.031  
0 0.137 0.139 0.138    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Q&A and Customer Reviews


What is the antibody pair and the recombinant protein.
Is there any possibility to purchase those componets separatedly? CSB-EQ027465PI

Thanks for your inquiry!
Here is the information of the antibody and protein for CSB-EQ027465PI :
Coated anti: Goat polyclonal antibody
Detection anti: Goat polyclonal antibody
Standard: 293 cell-derived recombinant protein
So sorry that the components of our elisa kits are not sold separately.

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