Canine Procalcitonin(PCT)ELISA Kit

Code CSB-E17400c
Size 96T,5×96T,10×96T How to order?
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Dog PCT ELISA Kit was designed for the quantitative measurement of Dog PCT protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 46.88 pg/mL-3000 pg/mL and the sensitivity is 11.72 pg/mL.

Target Name Procalcitonin(PCT)
Abbreviation PCT
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, cell culture supernates
Detection Range 46.88 pg/mL-3000 pg/mL
Sensitivity 11.72 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of canine PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 86
Range % 83-90
1:2 Average % 95
Range % 91-99
1:4 Average % 91
Range % 85-97
1:8 Average % 83
Range % 80-86
Recovery
The recovery of canine PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 87 84-93
EDTA plasma (n=4) 104 101-107
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
3000 2.839 2.901 2.870 2.772
1500 2.341 2.302 2.322 2.224
750 1.602 1.657 1.630 1.532
375 0.920 0.972 0.946 0.848
187.5 0.425 0.408 0.417 0.319
93.75 0.252 0.261 0.257 0.159
46.88 0.144 0.152 0.148 0.050
0 0.099 0.096 0.098  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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