Rabbit Procalcitonin (PCT) ELISA Kit

Code CSB-E12875Rb
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Procalcitonin (PCT)
Abbreviation PCT
Species Oryctolagus cuniculus (Rabbit)
Sample Types serum, plasma
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of rabbit PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 86-102  
1:2 Average % 86  
Range % 81-99  
1:4 Average % 95  
Range % 90-103  
1:8 Average % 93  
Range % 84-100  
The recovery of rabbit PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 86-94  
EDTA plasma (n=4) 96 92-102  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.860 2.786 2.823 2.706  
500 2.447 2.319 2.383 2.266  
250 1.643 1.588 1.616 1.499  
125 0.892 0.907 0.900 0.783  
62.5 0.463 0.452 0.458 0.341  
31.2 0.313 0.321 0.317 0.200  
15.6 0.221 0.212 0.217 0.100  
0 0.119 0.115 0.117    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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