Pig Procalcitonin,PCT ELISA Kit

Pig Procalcitonin,PCT ELISA Kit

Product Details

Citations

Customer Reviews and Q&A

Product Details

Citations

Related Products

Customer Reviews and Q&A

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Pig Procalcitonin,PCT ELISA Kit

Citations (2) Protocol MSDS

Code	CSB-E15004p
Size	96T,5×96T,10×96T
Price	Request a Quote
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Product Details
Citations (2)
Related Products
Customer Reviews and Q&A

Target Name

Procalcitonin,PCT

Abbreviation

PCT

Species

Sus scrofa (Pig)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39 pg/mL-2500 pg/mL

Sensitivity

9.75 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Signal Transduction

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	82-92
1:2	Average %	95
	Range %	88-98
1:4	Average %	92
	Range %	89-96
1:8	Average %	93
	Range %	88-98

Recovery

The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-96
EDTA plasma (n=4)	96	92-99

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2500	2.165	2.145	2.155	2.056
1250	1.697	1.678	1.688	1.589
625	1.298	1.223	1.261	1.162
312.5	0.860	0.854	0.857	0.758
156	0.516	0.501	0.509	0.410
78	0.381	0.371	0.376	0.277
39	0.235	0.222	0.229	0.130
0	0.100	0.098	0.099

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This Pig PCT ELISA Kit was designed for the quantitative measurement of Pig PCT protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 39 pg/mL-2500 pg/mL and the sensitivity is 9.75 pg/mL.

Histologic Changes Of Porcine Portal Vein Anastomosis after Electrochemotherapy with Bleomycin U Stupan,SSRN,2023
Initial Immune Response in Escherichia coli, Staphylococcus aureus, and Candida albicans Bacteremia Vaios Spyropoulos, et al,Inflammation,2019

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Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.