Pig Procalcitonin,PCT ELISA Kit

Instructions
Code CSB-E15004p
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name Procalcitonin,PCT
Abbreviation PCT
Species Sus scrofa (Pig)
Sample Types serum, plasma, tissue homogenates
Detection Range 39 pg/mL-2500 pg/mL
Sensitivity 9.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of pig PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %82-92
1:2Average %95
Range %88-98
1:4Average %92
Range %89-96
1:8Average %93
Range %88-98
Recovery
The recovery of pig PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9489-96
EDTA plasma (n=4)9692-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
25002.165 2.145 2.155 2.056
12501.697 1.678 1.688 1.589
6251.298 1.223 1.261 1.162
312.50.860 0.854 0.857 0.758
1560.516 0.501 0.509 0.410
780.381 0.371 0.376 0.277
390.235 0.222 0.229 0.130
00.100 0.098 0.099  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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