Rat procalcitonin (PCT) ELISA kit

Instructions
Code CSB-E13419r
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name procalcitonin (PCT)
Abbreviation PCT
Species Rattus norvegicus (Rat)
Sample Types serum, plasma, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of rat PCT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %84
Range %80-88
1:2Average %96
Range %91-103
1:4Average %97
Range %91-105
1:8Average %94
Range %89-99
Recovery
The recovery of rat PCT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10198-107
EDTA plasma (n=4)9792-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.655 2.554 2.605 2.516
10001.828 1.727 1.778 1.689
5000.973 0.996 0.985 0.896
2500.548 0.559 0.554 0.465
1250.302 0.312 0.307 0.218
62.50.244 0.233 0.239 0.150
31.250.147 0.142 0.145 0.056
00.089 0.088 0.089  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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