Dog High mobility group protein B1(HMGB1) ELISA kit

Instructions
Code CSB-EL010553DO
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name high-mobility group box 1
Alternative Names HMGB1 ELISA Kit; High mobility group protein B1 ELISA Kit; High mobility group protein 1 ELISA Kit; HMG-1 ELISA Kit
Abbreviation HMGB1
Uniprot No. Q6YKA4
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Epigenetics and Nuclear Signaling
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog HMGB1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 88-97
1:2 Average % 89
Range % 85-93
1:4 Average % 94
Range % 90-98
1:8 Average % 103
Range % 99-107
Recovery
The recovery of dog HMGB1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 85 80-90
EDTA plasma (n=4) 97 92-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
20 2.673 2.631 2.652 2.526
10 1.549 1.518 1.534 1.408
5 0.904 0.952 0.928 0.802
2.5 0.612 0.629 0.621 0.495
1.25 0.396 0.407 0.402 0.276
0.625 0.281 0.269 0.275 0.149
0.312 0.157 0.165 0.161 0.035
0 0.128 0.124 0.126  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days

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 Q&A
Q:

There is talk that dog HMGB1 is homologous to human HMGB1. Do you have any data or knowledge on this point?

A:
Thanks for your inquiry!
If you want to test human samples, we suggest you use the kit for human.
Sorry we didn't test the cross reactivity with human before and don't suggest you use this kit to test human samples because there is matrix interference and the antibody's specificity is different. Although the homology is very high, it can not be ensured 100%.

Target Data

Function Multifunctional redox sensitive protein with various roles in different cellular compartments. In the nucleus is one of the major chromatin-associated non-histone proteins and acts as a DNA chaperone involved in replication, transcription, chromatin remodeling, V(D)J recombination, DNA repair and genome stability. Proposed to be an universal biosensor for nucleic acids. Promotes host inflammatory response to sterile and infectious signals and is involved in the coordination and integration of innate and adaptive immune responses. In the cytoplasm functions as sensor and/or chaperone for immunogenic nucleic acids implicating the activation of TLR9-mediated immune responses, and mediates autophagy. Acts as danger associated molecular pattern (DAMP) molecule that amplifies immune responses during tissue injury. Released to the extracellular environment can bind DNA, nucleosomes, IL-1 beta, CXCL12, AGER isoform 2/sRAGE, lipopolysaccharide (LPS) and lipoteichoic acid (LTA), and activates cells through engagement of multiple surface receptors. In the extracellular compartment fully reduced HMGB1 (released by necrosis) acts as a chemokine, disulfide HMGB1 (actively secreted) as a cytokine, and sulfonyl HMGB1 (released from apoptotic cells) promotes immunological tolerance. Has proangiogenic activity. May be involved in platelet activation. Binds to phosphatidylserine and phosphatidylethanolamide. Bound to RAGE mediates signaling for neuronal outgrowth. May play a role in accumulation of expanded polyglutamine (polyQ) proteins.
Gene References into Functions
  1. The observed HMGB1 release kinetics showed a clearly time-dependent manner with a peak release 24h after TNF-alpha stimulation, while stimulation with IFN-gamma had only small effects on the HMGB1 release. PMID: 22154216
  2. RAGE and HMGB1 expression down-regulation in canine disseminated histiocytic sarcoma points to a role in the progression of histiocytic disorders PMID: 21617208
Subcellular Location Nucleus, Chromosome, Cytoplasm, Secreted, Cell membrane, Peripheral membrane protein, Extracellular side, Endosome, Endoplasmic reticulum-Golgi intermediate compartment
Protein Families HMGB family
Database Links

KEGG: cfa:403170

STRING: 9615.ENSCAFP00000009862

UniGene: Cfa.416

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