Dog Interleukin 1β (IL- 1β) ELISA Kit

Code CSB-E13836c
Size 96T,5×96T,10×96T
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Product Details

Target Name
interleukin 1, beta
Alternative Names
IL1BInterleukin-1 beta ELISA Kit; IL-1 beta ELISA Kit
Abbreviation
IL1B
Uniprot No.
Species
Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types
serum, plasma, cell culture supernates, tissue homogenates
Detection Range
7.8 pg/mL-500 pg/mL
Sensitivity
1.95 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-1β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %92
Range %83-100
1:2Average %94
Range %91-99
1:4Average %95
Range %90-105
1:8Average %94
Range %86-100
Recovery
The recovery of dog IL-1β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-102
EDTA plasma (n=4)9790-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
5002.585 2.675 2.630 2.512
2502.319 2.412 2.366 2.248
1251.957 1.972 1.965 1.847
62.51.432 1.453 1.443 1.325
31.250.744 0.719 0.732 0.614
15.630.517 0.508 0.513 0.395
7.80.385 0.377 0.381 0.263
00.118 0.117 0.118  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Dog IL1B ELISA Kit was designed for the quantitative measurement of Dog IL1B protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 7.8 pg/mL-500 pg/mL and the sensitivity is 1.95 pg/mL .

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Target Background

Function
(From Uniprot)
Potent proinflammatory cytokine. Initially discovered as the major endogenous pyrogen, induces prostaglandin synthesis, neutrophil influx and activation, T-cell activation and cytokine production, B-cell activation and antibody production, and fibroblast proliferation and collagen production. Promotes Th17 differentiation of T-cells. Synergizes with IL12/interleukin-12 to induce IFNG synthesis from T-helper 1 (Th1) cells. Plays a role in angiogenesis by inducing VEGF production synergistically with TNF and IL6.
Subcellular Location
Cytoplasm, cytosol. Lysosome. Secreted, extracellular exosome. Secreted.
Protein Families
IL-1 family
Database Links
CUSABIO guaranteed quality
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