Dog Interleukin-8,IL-8 ELISA Kit

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Dog Interleukin-8,IL-8 ELISA Kit

Name: Dog Interleukin-8,IL-8 ELISA Kit
Brand: CUSABIO
SKU: CSB-E08052c

^Instructions

Code	CSB-E08052c
Size	96T,5×96T,10×96T
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Product Details
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Target Data

Target Name

interleukin 8

Alternative Names

CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic

Abbreviation

IL8

Uniprot No.

P41324

Species

Canis lupus familiaris (Dog) (Canis familiaris)

Sample Types

serum, plasma, cell culture supernates, tissue homogenates

Detection Range

0.312 ng/mL-20 ng/mL

Sensitivity

0.078 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	99
	Range %	95-105
1:4	Average %	88
	Range %	85-93
1:8	Average %	90
	Range %	86-97

Recovery

The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	88	85-95

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	3.221	3.295	3.258	3.157
10	2.523	2.711	2.617	2.516
5	1.791	1.930	1.861	1.760
2.5	1.035	1.045	1.040	0.939
1.25	0.466	0.485	0.476	0.375
0.625	0.249	0.259	0.254	0.153
0.312	0.157	0.167	0.162	0.061
0	0.103	0.099	0.101

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

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Synergistic Action of IL-8 and BoneMarrow Concentrate on Cartilage Regeneration Through Upregulation of Chondrogenic Transcription Factors Dong Suk Yoon. et al,Tissue Engineering Part A.,2016

Function	IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Subcellular Location	Secreted
Protein Families	Intercrine alpha (chemokine CxC) family
Database Links	KEGG: cfa:403850 STRING: 9615.ENSCAFP00000004516 UniGene: Cfa.3510

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

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