Dog Interleukin-8,IL-8 ELISA Kit

Instructions
Code CSB-E08052c
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name interleukin 8
Alternative Names CXCL8, GCP-1, GCP1, LECT, LUCT, LYNAP, MDNCF, MONAP, NAF, NAP-1, NAP1, T cell chemotactic factor|beta-thromboglobulin-like protein|chemokine (C-X-C motif) ligand 8|emoctakin|granulocyte chemotactic
Abbreviation IL8
Uniprot No. P41324
Species Canis lupus familiaris (Dog) (Canis familiaris)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.312 ng/mL-20 ng/mL
Sensitivity 0.078 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of dog IL-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %93
Range %89-97
1:2Average %99
Range %95-105
1:4Average %88
Range %85-93
1:8Average %90
Range %86-97
Recovery
The recovery of dog IL-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9086-94
EDTA plasma (n=4)8885-95
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
203.221 3.295 3.258 3.157
102.523 2.711 2.617 2.516
51.791 1.930 1.861 1.760
2.51.035 1.045 1.040 0.939
1.250.466 0.485 0.476 0.375
0.6250.249 0.259 0.254 0.153
0.3120.157 0.167 0.162 0.061
00.103 0.099 0.101
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function IL-8 is a chemotactic factor that attracts neutrophils, basophils, and T-cells, but not monocytes. It is also involved in neutrophil activation. It is released from several cell types in response to an inflammatory stimulus.
Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: cfa:403850

STRING: 9615.ENSCAFP00000004516

UniGene: Cfa.3510

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