Code | CSB-E11904h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human ADAM9 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 96 | ||||
Range % | 9-101 | |||||
1:2 | Average % | 87 | ||||
Range % | 84-92 | |||||
1:4 | Average % | 95 | ||||
Range % | 92-97 | |||||
1:8 | Average % | 93 | ||||
Range % | 89-99 |
The recovery of human ADAM9 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 90 | 87-93 | ||||
EDTA plasma (n=4) | 93 | 89-95 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
|
The ELISA Kit is designed for quantitatively measuring human ADAM9 levels in samples, including serum, plasma, tissue homogenates, cell culture supernates, or ascitic fluid. It uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of ADAM9 in samples. The color intensity is measured at 450 nm via a microplate reader.
ADAM9 is a metzincin cell-surface protease involved in several biological processes such as myogenesis, fertilization, cell migration, inflammatory response, proliferation, and cell-cell interactions. It is involved in inflammation, degenerative diseases, and cancer. In inflammation, ADAM9 contributes to monocyte fusion, mediating the conversion of monocytes-macrophages to multinucleated giant cells (MGCs) in response to foreign bodies or bacteria. The generated granulomatous lesions help to isolate the pathogens and also enhance phagocytotic activity. ADAM9 has been found over-expressed in several solid tumors such as glioma, melanoma, prostate cancer, pancreatic ductal adenocarcinoma, as well as gastric, breast, lung, and liver cancers. Upregulation of ADAM9 is associated with tumor aggressiveness and poor prognosis.
There are currently no reviews for this product.