Product Details

Target Name

chemokine (C-C motif) ligand 16

Alternative Names

C-C motif chemokine 16 ELISA Kit; CCL16 ELISA Kit; CCL16_HUMAN ELISA Kit; Chemokine (C-C motif) ligand 16 ELISA Kit; Chemokine CC-4 ELISA Kit; Chemokine LEC ELISA Kit; CKb12 ELISA Kit; HCC-4 ELISA Kit; HCC4 ELISA Kit; IL-10-inducible chemokine ELISA Kit; IL10 inducible chemokine ELISA Kit; IL10 inducible chemokine ELISA Kit; ILINCK ELISA Kit; LCC-1 ELISA Kit; LCC1 ELISA Kit; Liver CC chemokine 1 precursor ELISA Kit; Liver expressed chemokine ELISA Kit; Liver-expressed chemokine ELISA Kit; LMC ELISA Kit; Lymphocyte and monocyte chemoattractant ELISA Kit; Monotactin 1 ELISA Kit; Monotactin-1 ELISA Kit; MTN-1 ELISA Kit; Mtn1 ELISA Kit; NCC-4 ELISA Kit; NCC4 ELISA Kit; New CC chemokine 4 ELISA Kit; SCYA16 ELISA Kit; SCYL4 ELISA Kit; Small inducible cytokine A16 precursor ELISA Kit; Small inducible cytokine A16 precursor ELISA Kit; Small inducible cytokine subfamily A (Cys Cys) member 16 ELISA Kit; Small-inducible cytokine A16 ELISA Kit

Abbreviation

CCL16

Uniprot No.

O15467

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

39.06 pg/mL-2500 pg/mL

Sensitivity

9.77 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
	Intra-Assay Precision			Inter-Assay Precision
Sample	1	2	3	1	2	3
n	20	20	20	20	20	20
Mean(pg/ml)	312.528	312.182	312.923	313.120	312.775	312.676
SD	0.038	0.038	0.027	0.042	0.040	0.045
CV(%)	5.574	5.580	3.956	6.150	5.863	6.598

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CCL16 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:2

Average %

Range %

80-95

1:4

Average %

Range %

91-105

1:8

Average %

Range %

92-110

1:16

Average %

Range %

86-99

Recovery

The recovery of human CCL16 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	89-98
EDTA plasma (n=4)	94	91-100

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.


pg/ml	OD1	OD2	Average	Corrected
2500	2.795	2.880	2.838	2.680
1250	2.147	2.142	2.145	1.987
625	1.317	1.426	1.372	1.214
312.5	0.647	0.667	0.657	0.499
156.25	0.367	0.325	0.346	0.188
78.13	0.227	0.244	0.236	0.078
39.06	0.174	0.179	0.177	0.019
0	0.159	0.157	0.158

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

This human CCL16 ELISA kit employs the quantitative sandwich enzyme immunoassay technique to measure the levels of human CCL16 in multiple samples, including serum, plasma, or tissue homogenates. It also uses the enzyme-substrate chromogenic reaction to visualize and analyze the analyte levels through the color intensity. The intensity of the colored product is in direct proportion to the CCL16 levels in the sample and is measured at 450 nm through a microplate reader.

CCL16 is a chemokine predominantly expressed in the liver but is also found in the blood and brain. It plays important roles in immune response and angiogenesis. CCL16 specifically attracts lymphocytes, dendritic cells, and monocytes and increases their adhesive properties. CCL16 also possesses myelosuppressive activity. A study has shown that CCL16 binds explicitly to CCR2, which in turn activates the AKT/GSK3β signaling and promotes β-catenin nuclear translocation, leading to enhanced cancer cell stemness. CCL16 also leads to the migration of cancer cells if they express CCR1. On the other hand, CCL16 enhances the anti-cancer effects of cytotoxic T and dendritic cells (DC) lymphocytes.

Code	CSB-EL004779HU
Size	96T,5×96T,10×96T
Price	Request a Quote
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Human C-C motif chemokine 16(CCL16) ELISA kit

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