Human CD70 antigen(CD70) ELISA kit

Code CSB-EL004954HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P32970
Lead Time 7-14 working days
Abbreviation CD70
Target Name CD70 molecule
Alias CD27L, CD27LG, TNFSF7, CD27 ligand|CD70 antigen|Ki-24 antigen|surface antigen CD70|tumor necrosis factor (ligand) superfamily, member 7|tumor necrosis factor ligand superfamily, member 7
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This cytokine is a ligand for TNFRSF27/CD27. It is a surface antigen on activated, but not on resting, T and B lymphocytes. It induces proliferation of costimulated T cells, enhances the generation of cytolytic T cells, and contributes to T cell activation. This cytokine is also reported to play a role in regulating B-cell activation, cytotoxic function of natural killer cells, and immunoglobulin sythesis.
HGNC 11937
RGD 1588573
MGI 1195273
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD70 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %94
Range %90-98
1:2Average %92
Range %89-97
1:4Average %93
Range %90-96
1:8Average %96
Range %90-101
Recovery
The recovery of human CD70 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 10095-105
EDTA plasma (n=4)9790-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.196 2.189 2.193 2.085
51.699 1.671 1.685 1.577
2.51.166 1.182 1.174 1.066
1.250.766 0.712 0.739 0.631
0.6250.503 0.526 0.515 0.407
0.3120.352 0.327 0.340 0.232
0.1560.206 0.216 0.211 0.103
00.107 0.109 0.108  
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Function Cytokine that binds to CD27. Plays a role in T-cell activation. Induces the proliferation of costimulated T-cells and enhances the generation of cytolytic T-cells.
Subcellular Location Membrane, Single-pass type II membrane protein
Protein Families Tumor necrosis factor family
Database Links

HGNC: 11937

OMIM: 602840

KEGG: hsa:970

STRING: 9606.ENSP00000245903

UniGene: Hs.501497

Q&A and Customer Reviews

Customer Reviews
  • Tested application: ELISA
    Test sample: Serum
    Species: Human
    Review:Used this kit to test a variety of serum samples. Instructions were easy to follow and sufficient reagent is provided to test a variety of conditions. I wish i had more info on the standard used in the kit so it could easily be compared to other vendor protein options tested! Overall impressed with the packaging and ease of use.

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