Human High Molecular Weight Adiponectin(HMW Adiponectin)ELISA Kit

Instructions
Code CSB-E13400h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name High Molecular Weight Adiponectin(HMW Adiponectin)
Abbreviation HMW Adiponectin
Uniprot No. Q15848
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human HMW adiponectin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %87
Range %84-91
1:200Average %95
Range %90-100
1:400Average %99
Range %95-104
1:800Average %101
Range %96-106
Recovery
The recovery of human HMW adiponectin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9995-104
EDTA plasma (n=4)9287-98
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
2002.169 2.117 2.143 2.040
1001.679 1.658 1.669 1.566
501.245 1.231 1.238 1.135
250.832 0.869 0.851 0.748
12.50.513 0.547 0.530 0.427
6.250.345 0.367 0.356 0.253
3.120.203 0.212 0.208 0.105
00.102 0.104 0.103  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
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Target Data

Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Involvement in disease Adiponectin deficiency (ADPND); Diabetes mellitus, non-insulin-dependent (NIDDM)
Subcellular Location Secreted
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Database Links

HGNC: 13633

OMIM: 125853

KEGG: hsa:9370

STRING: 9606.ENSP00000320709

UniGene: Hs.80485

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