Human adiponectin,ADP ELISA Kit

Instructions
Code CSB-E07270h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name adiponectin, C1Q and collagen domain containing
Alternative Names ACDC, ACRP30, ADIPQTL1, ADPN, APM-1, APM1, GBP28, adiponectin, OTTHUMP00000210047|OTTHUMP00000210048|adipocyte, C1Q and collagen domain containing|adipocyte, C1Q and collagen domain-containing|adipo
Abbreviation ADP
Uniprot No. Q15848
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 1.562 ng/mL-100 ng/mL
Sensitivity 1.102 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Citations

Q&A and Customer Reviews

 Q&A
Q:

Please confirm whether this kit can be used with Saliva samples and also suggest suitable protocol for saliva sample preparation.

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test human saliva samples, you can do a pretest first.
Here is the processing method on saliva sample preparation.You can take it as reference:Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles. Recommend to use fresh saliva samples.
 Customer Reviews

Target Data

Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Involvement in disease Adiponectin deficiency (ADPND); Diabetes mellitus, non-insulin-dependent (NIDDM)
Subcellular Location Secreted
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Database Links

HGNC: 13633

OMIM: 125853

KEGG: hsa:9370

STRING: 9606.ENSP00000320709

UniGene: Hs.80485

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.