Human adiponectin,ADP ELISA Kit

Instructions
Code CSB-E07270h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The Human adiponectin (ADP) ELISA Kit allows for the in vitro quantitative determination of ADP concentrations in serum, plasma, cell culture supernates, tissue homogenates, or urine. This kit exclusively recognizes human ADP. Adiponectin is the most abundant peptide secreted mainly by adipocytes. It is an insulin sensitizer, and decreased adiponectin levels are associated with insulin resistance and metabolic syndrome. Adiponectin interacting with its receptors Adipo R1 and R2 activates multiple signaling pathways, including IRS1/2, AMPK, and p38 MAPK. Activation of IRS1/2 by adiponectin signaling is the main mechanism by which adiponectin sensitizes insulin action in insulin-responsive tissues.

The detection mechanism of this kit is based on the Sandwich-ELISA technique. ADP in the sample is bound to the capture antibody immobilized on the microtiter plate and then sandwiched with a Biotin-labeled ADP antibody. The solution color develops into blue after the ordinal addition of HRP-avidin and TMB. The color development is terminated after adding the stop solution, and the color turns from blue to yellow. The color intensity is positively correlated with ADP content in the sample. The kit has been quality controlled with high sensitivity, strong specificity, good linearity, precision less than 10%, recovery with 85%-102%, and high lot-to-lot consistency. Refer to the product manual for more information.

Target Name adiponectin, C1Q and collagen domain containing
Alternative Names 30 kDa adipocyte complement related protein ELISA Kit; 30 kDa adipocyte complement-related protein ELISA Kit; ACDC ELISA Kit; Acrp 30 ELISA Kit; ACRP30 ELISA Kit; ADIPO_HUMAN ELISA Kit; Adipocyte ELISA Kit; Adipocyte C1q and collagen domain containing protein ELISA Kit; Adipocyte complement related 30 kDa protein ELISA Kit; Adipocyte complement related protein of 30 kDa ELISA Kit; Adipocyte complement-related 30 kDa protein ELISA Kit; adipocyte-specific secretory protein ELISA Kit; Adiponectin ELISA Kit; Adiponectin precursor ELISA Kit; adiponectin; C1Q and collagen domain containing ELISA Kit; Adipoq ELISA Kit; Adipose most abundant gene transcript 1 ELISA Kit; Adipose most abundant gene transcript 1 protein ELISA Kit; Adipose specific collagen like factor ELISA Kit; ADIPQTL1 ELISA Kit; ADPN ELISA Kit; APM 1 ELISA Kit; apM-1 ELISA Kit; APM1 ELISA Kit; C1q and collagen domain-containing protein ELISA Kit; GBP 28 ELISA Kit; GBP28 ELISA Kit; Gelatin binding protein ELISA Kit; Gelatin binding protein 28 ELISA Kit; gelatin-binding protein 28 ELISA Kit; Gelatin-binding protein ELISA Kit; OTTHUMP00000210047 ELISA Kit
Abbreviation ADP
Uniprot No. Q15848
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, urine
Detection Range 1.562 ng/mL-100 ng/mL
Sensitivity 1.102 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision

 

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

 

Linearity

 

To assess the linearity of the assay, samples were spiked with high concentrations of human ADP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

 

Typical Data

 

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

 

Materials provided
  • A micro ELISA plate --- The 96-well plate has been pre-coated with an anti-human ADP antibody. This dismountable microplate can be divided into 12 x 8 strip plates.
  • Two vials lyophilized standard --- Dilute a bottle of the standard at dilution series, read the OD values, and then draw a standard curve.
  • One vial Biotin-labeled ADP antibody (100 x concentrate) (120 μl/bottle) ---Act as the detection antibody.
  • One vial HRP-avidin (100 x concentrate) (120 μl/bottle) --- Bind to the detection antibody and react with the TMB substrate to make the solution chromogenic.
  • One vial Biotin-antibody Diluent (15 ml/bottle) ---Dilute the high concentration Biotin-antibody to an appropriate working solution.
  • One vial HRP-avidin Diluent (15 ml/bottle) ---Dilute the high concentration HRP-avidin solution to an appropriate solution.
  • One vial Sample Diluent (50 ml/bottle)---Dilute the sample to an appropriate concentration.
  • One vial Wash Buffer (25 x concentrate) (20 ml/bottle) --- Wash away unbound or free substances.
  • One vial TMB Substrate (10 ml/bottle) --- Act as the chromogenic agent. TMB interacts with HRP, eliciting the solution turns blue.
  • One vial Stop Solution (10 ml/bottle) --- Stop the color reaction. The solution color immediately turns from blue to yellow.
  • Four Adhesive Strips (For 96 wells) --- Cover the microplate when incubation.
  • An instruction manual
Materials not provided
  • A microplate reader capable of measuring absorbance at 450 nm, with the correction wavelength set at 540 nm or 570 nm.
  • An incubator can provide stable incubation conditions up to 37°C±5°C.
  • Centrifuge
  • Vortex
  • Squirt bottle, manifold dispenser, or automated microplate washer
  • Absorbent paper for blotting the microtiter plate
  • 50-300ul multi-channel micropipette
  • Pipette tips
  • Single-channel micropipette with different ranges
  • 100ml and 500ml graduated cylinders
  • Deionized or distilled water
  • Timer
  • Test tubes for dilution
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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 Q&A
Q:

Please confirm whether this kit can be used with Saliva samples and also suggest suitable protocol for saliva sample preparation.

A:
Thanks for your inquiry. Usually we suggest you test the sample types mentioned in the kit manual. If you want to test human saliva samples, you can do a pretest first.
Here is the processing method on saliva sample preparation.You can take it as reference:Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately or aliquot and store samples at -20°C. Avoid repeated freeze-thaw cycles. Recommend to use fresh saliva samples.

Target Data

Function Important adipokine involved in the control of fat metabolism and insulin sensitivity, with direct anti-diabetic, anti-atherogenic and anti-inflammatory activities. Stimulates AMPK phosphorylation and activation in the liver and the skeletal muscle, enhancing glucose utilization and fatty-acid combustion. Antagonizes TNF-alpha by negatively regulating its expression in various tissues such as liver and macrophages, and also by counteracting its effects. Inhibits endothelial NF-kappa-B signaling through a cAMP-dependent pathway. May play a role in cell growth, angiogenesis and tissue remodeling by binding and sequestering various growth factors with distinct binding affinities, depending on the type of complex, LMW, MMW or HMW.
Gene References into Functions
  1. Serum adiponectin correlates negatively with bone density in type 2 diabetic osteoporotic postmenopausal women. PMID: 29853264
  2. The 276G gene of adiponectin may be a susceptibility gene of CHD, and the genotype GG may be related to the severity of this disease. PMID: 30311881
  3. Alongside insulin resistance, increased sympathetic activity is associated with and may modulate HMW adiponectin levels in women with polycystic ovary syndrome. PMID: 29428305
  4. The adiponectin +276G/T polymorphism may be associated with the development of Kawasaki disease. PMID: 30022756
  5. Genetic polymorphisms in the adiponectin gene of recipients (but not donors) are associated with early de novo posttransplant hepatic steatosis. PMID: 29863454
  6. Expression of adiponectin inhibit the proliferation of colorectal cancer HCT116 cells and induce cell arrest at G1/G0 phase and promote apoptosis. PMID: 29773108
  7. Adiponectin was negatively related only in obese subjects PMID: 30070872
  8. ADIPOQ gene SNP rs182052 was associated with antipsychotic-induced weight gain. PMID: 28327672
  9. the current study indicated that the adiponectin expression levels were higher in the osteoarthritis patients than in the healthy controls. PMID: 30115130
  10. Serum total adiponectin and gender-specific ADIPOQ variants predict CHD and IS, but not HF, independently of other risk factors in community-based patients with T2D. PMID: 29969725
  11. severely obese patients, adiponectin gene expression in visceral adipose tissue (VAT) is negatively correlated with serum levels of uric acid and is an independent determinant, together with anthropometric parameters of visceral obesity and serum adiponectin levels, of insulin resistance. PMID: 29645064
  12. Adiponectin and colon cancer: evidence for inhibitory effects on viability and migration of human colorectal cell lines. PMID: 29446048
  13. Our meta-analysis suggests that the rs1501299 polymorphism may play a protective role in hypertension in Caucasian subgroup PMID: 28181566
  14. Studied the association of SNPs in the adiponectin gene with coronary artery disease in 277 Tunisian patients with angiographically confirmed coronary artery disease. PMID: 29633340
  15. Increased cardiovascular disease risk is associated with ADIPOQ SNPs, rs266729 and rs2241766, but not associated with rs1501299 (meta-analysis). PMID: 29807528
  16. High ADIPOQ expression is associated with hypothyroidism. PMID: 28728192
  17. adiponectin 45T/G polymorphism is associated with diabetic cardiovascular complication in type 2 diabetes PMID: 29686595
  18. preoperative adiponectin levels correlate with postoperative complications after cancer surgery PMID: 29141566
  19. women with gestational diabetes showed significantly low adiponectin and high resistin levels when compared with control group. PMID: 29207892
  20. Late improvement of low-density lipoprotein cholesterol and adiponectin after transplant suggested that kidney transplant recipients may still have risk of cardiovascular events, especially in the first years. PMID: 27267514
  21. Findings suggest a significant association between variants in COL13A1, ADIPOQ, SAMM50, and PNPLA3, and risk of NAFLD/elevated transaminase levels in Mexican adults with an admixed ancestry. PMID: 29307798
  22. fetuin a to adiponectin ratio is more strongly associated with metabolic syndrome and its components than fetuin-A or adiponectin alone PMID: 28779425
  23. component of mid-trimester amniotic fluid; its concentration varies with maternal body mass index and subsequent development of preterm birth, fetal growth restriction, and respiratory distress syndrome PMID: 28708576
  24. Data suggest that first trimester measurement of adiponectin and 1,5-anhydroglucitol are potential early serum biomarkers for later onset of gestational diabetes; both serum levels are lower in women who later develop gestational diabetes than in women who do not. This study was conducted in Dublin, Ireland. PMID: 29328940
  25. Worse nutritional state in de novo heart failure patients is associated with higher adiponectin plasma levels. Their levels were upregulated in adipose cells after being nutrients-starved. These results may help us to understand the adiponectin paradox in heart failure. PMID: 29559829
  26. Adiponectin may be considered a biomarker of metabolic compensation, inversely associated with chronic low-grade inflammation. Circulating adiponectin is also associated with lower risk of adverse cardivascular events in patients with severe carotid stenosis. PMID: 29305337
  27. Review/Meta-analysis: circulating adiponectin levels were significantly higher in patients with rheumatoid arthritis, but there was no correlation with disease activity. PMID: 28205390
  28. ADIPOQ rs1501299 gene polymorphism is associated with an increased risk of new-onset diabetes mellitus after liver transplantation. PMID: 29291779
  29. Serum levels of adiponectin were significantly and negatively associated with bone mineral density in patients with knee osteoarthritis. PMID: 29343264
  30. Data suggest that higher consumption of bread and dairy products along with low intake of rice may be associated with increased serum adiponectin in Japanese women. PMID: 28637371
  31. Data suggest that plasma adiponectin levels in obese women with low REE (resting energy expenditure) are significantly lower compared to obese women with low REE and compared to normal weight women. PMID: 29017362
  32. Elevated plasma adiponectin concentrations were associated with both clinical and radiological disease severity in knee osteoarthritis patients. PMID: 26544540
  33. High ADIPOQ expression is associated with osteoporosis and arterial stiffness. PMID: 28646476
  34. Higher adiponectin levels were associated with a more favourable development of cardiovascular autonomic function in individuals with type 2 diabetes independently of multiple confounders. PMID: 29195493
  35. Low ADIPOQ expression is associated with obesity. PMID: 28087246
  36. Findings indicate that blood adiponectin concentration is more likely to be an epiphenomenon in the context of metabolic disease than a key determinant. PMID: 29237687
  37. elevated circulating total adiponectin levels were associated with lower 10-year cardiovascular disease risk in adults without previous cardiovascular disease PMID: 29039145
  38. in lumbar facet joint osteoarthritis, expression in the subchrondral bone decreased as the degeneration increased PMID: 29100514
  39. Data suggest that transient increases in plasma adiponectin levels and transient decreases in plasma leptin levels occur following high-intensity interval exercise in overweight and obese young women. These studies were conducted in Turkey in formerly sedentary women ages 20-30 years. PMID: 28857629
  40. Adipolin and adiponectin are cytokines that exert substantial impact on obesity, progression of atherosclerosis, insulin resistance, and glucose metabolism. PMID: 29452073
  41. This meta-analysis demonstrates that serum/plasma levels of adiponectin in type 1 diabetes mellitus, rheumatoid arthritis, and systemic lupus erythematosus patients were higher than those in normal controls, but not in ankylosing spondylitis patients. PMID: 28702765
  42. Data suggest that, in overweight and obese subjects, weight reduction induces changes in chemerin serum levels in parallel with adiponectin plasma levels; chemerin is down-regulated and adiponectin is up-regulated in both insulin-resistant and insulin-sensitive subjects after weight reduction; however, only changes in chemerin in insulin-sensitive subjects were found to be statistically significant. PMID: 28323510
  43. ADIPOQ rs182052 AA allele is associated with obesity. PMID: 28947843
  44. The current investigation demonstrated that, unlike the reduction in the levels of all the adiponectin isoforms and the high molecular weight (HMW) / total adiponectin (TA) ratio, an increased low molecular weight (LMW) / TA ratio was associated with T2D through its relation to insulin resistance. PMID: 29494595
  45. This study showed that homeostatic model assessment (HOMA)-insulin resistance (IR) but not HOMA-adiponectin was appropriate for diagnosing IR. PMID: 29217381
  46. Data suggest that vitamin D deficiency (indicated by low maternal 25(OH)D concentrations) in overweight/obese pregnant women at high-risk of gestational diabetes is associated with increased cardiometabolic risk during pregnancy and with adverse pregnancy outcomes; these associations may be mediated by HMW-adiponectin. PMID: 28741856
  47. The depigmenting effect of adiponectin was mediated via AMPK activation, which induced the inhibitory phosphorylation of CREB-regulated transcription co-activators (CRTCs) and subsequent suppression of the novel CRTC/CREB pathway in melanocytes. PMID: 28481450
  48. In anorexia nervosa subjects, TT genotype in ADIPOQ c.276 polymorphism was significantly more frequent than in control group. PMID: 28759190
  49. Our data suggest that adiponectin and resistin are altered in non-obese chronic migraineurs. PMID: 27553954
  50. An increased proportion of trunk fat is associated with decreased adiponectin in relatively overweight children, and also demonstrated that girls with a normal volume of body fat that follows a more centralized distribution show decreased adiponectin. PMID: 26655234

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Involvement in disease Adiponectin deficiency (ADPND); Diabetes mellitus, non-insulin-dependent (NIDDM)
Subcellular Location Secreted
Tissue Specificity Synthesized exclusively by adipocytes and secreted into plasma.
Database Links

HGNC: 13633

OMIM: 125853

KEGG: hsa:9370

STRING: 9606.ENSP00000320709

UniGene: Hs.80485

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