Human IgG receptor FcRn large subunit p51(FCGRT) ELISA kit

Code CSB-EL008545HU
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P55899
Lead Time 7-14 working days
Abbreviation FCGRT
Target Name Fc fragment of IgG, receptor, transporter, alpha
Alias FCRN, alpha-chain, FcRn alpha chain|IgG receptor FcRn large subunit p51|immunoglobulin receptor, intestinal, heavy chain|major histocompatibility complex class I-like Fc receptor|neonatal Fc-recepto
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This gene encodes a receptor that binds the Fc region of monomeric immunoglobulin G. The encoded protein transfers immunoglobulin G antibodies from mother to fetus across the placenta. This protein also binds immunoglobulin G to protect the antibody from degradation. Alternative splicing results in multiple transcript variants.
HGNC 3621
RGD 61811
MGI 103017
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human FCGRT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:1Average %86
Range %80-90
1:2Average %96
Range %91-100
1:4Average %90
Range %87-96
1:8Average %91
Range %86-97
Recovery
The recovery of human FCGRT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9184-98
EDTA plasma (n=4)8885-93
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
10002.415 2.352 2.384 2.254
5001.895 1.794 1.845 1.715
2501.244 1.204 1.224 1.094
1250.753 0.739 0.746 0.616
62.50.447 0.424 0.436 0.306
31.20.346 0.319 0.333 0.203
15.60.211 0.196 0.204 0.074
00.132 0.127 0.130  
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Function Binds to the Fc region of monomeric immunoglobulins gamma
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily
Tissue Specificity Expressed in full-term placenta, heart, lung, liver, muscle, kidney, pancreas, and both fetal and adult small intestine.
Database Links

HGNC: 3621

OMIM: 601437

KEGG: hsa:2217

STRING: 9606.ENSP00000221466

UniGene: Hs.111903

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