FCGRT Antibody

Datasheet
Code CSB-PA008545EA01HU
See More Details Free Antibody trial simple
Size US$299Purchase it in Cusabio online store
(only available for customers from the US)
Uniprot No. P55899
Image
  • Western blot
    All lanes: FCGRT antibody at 2µg/ml
    Lane 1: HepG2 whole cell lysate
    Lane 2: Mouse liver tissue
    Lane 3: A549 whole cell lysate
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Western blot
    All lanes: FCGRT antibody at 5.3µg/ml
    Lane 1: Mouse liver tissue
    Lane 2: Mouse kidney tissue
    Secondary
    Goat polyclonal to rabbit IgG at 1/10000 dilution
    Predicted band size: 40 kDa
    Observed band size: 40 kDa

  • Immunohistochemistry of paraffin-embedded human placenta tissue using CSB-PA008545EA01HU at dilution of 1:100

  • Immunohistochemistry of paraffin-embedded human pancreatic cancer using CSB-PA008545EA01HU at dilution of 1:100

  • Immunofluorescent analysis of HepG2 cells using CSB-PA008545EA01HU at dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG(H+L)

  • IHC image of CSB-PA008545EA01HU diluted at 1:500 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

  • IHC image of CSB-PA008545EA01HU diluted at 1:500 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Protocols ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Immunogen Recombinant Human IgG receptor FcRn large subunit p51 protein (24-297AA)
Raised in Rabbit
Species Reactivity Human, Mouse
Tested Applications ELISA, WB, IHC, IF; Recommended dilution: WB:1:500-1:2000, IHC:1:20-1:1000, IF:1:50-1:200
Form Liquid
Conjugate Non-conjugated
Storage Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Purification Method >95%, Protein G purified
Isotype IgG
Clonality Polyclonal
Alias IgG receptor FcRn large subunit p51 (FcRn) (IgG Fc fragment receptor transporter alpha chain) (Neonatal Fc receptor), FCGRT, FCRN
Immunogen Species Human
Research Area Immunology
Target Names FCGRT
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
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Function Binds to the Fc region of monomeric immunoglobulins gamma
Subcellular Location Cell membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily
Tissue Specificity Expressed in full-term placenta, heart, lung, liver, muscle, kidney, pancreas, and both fetal and adult small intestine.
Database Links

HGNC: 3621

OMIM: 601437

KEGG: hsa:2217

STRING: 9606.ENSP00000221466

UniGene: Hs.111903

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