Code | CSB-E04601h-IS |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 87 | ||||
Range % | 82-94 | |||||
1:2 | Average % | 95 | ||||
Range % | 88-101 | |||||
1:4 | Average % | 92 | ||||
Range % | 87-98 | |||||
1:8 | Average % | 100 | ||||
Range % | 95-105 |
The recovery of human IL-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 105 | 97-111 | ||||
EDTA plasma (n=4) | 96 | 90-100 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
pg/ml | OD1 | OD2 | Average | Corrected | |||
200 | 3.074 | 2.934 | 3.004 | 2.879 | |||
100 | 2.111 | 2.167 | 2.139 | 2.015 | |||
50 | 1.264 | 1.328 | 1.296 | 1.172 | |||
25 | 0.793 | 0.819 | 0.806 | 0.682 | |||
12.5 | 0.413 | 0.421 | 0.417 | 0.293 | |||
6.25 | 0.263 | 0.253 | 0.258 | 0.134 | |||
3.12 | 0.169 | 0.174 | 0.171 | 0.047 | |||
0 | 0.124 | 0.125 | 0.124 |
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