Code | CSB-E12819h |
Size | $695 |
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Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. |
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-17A/IL-17 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
| Sample | Serum(n=4) | ||||
1:1 | Average % | 104 | ||||
Range % | 95-112 | |||||
1:2 | Average % | 99 | ||||
Range % | 94-105 | |||||
1:4 | Average % | 97 | ||||
Range % | 92-109 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-99 |
The recovery of human IL-17A/IL-17 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 96 | 89-102 | ||||
EDTA plasma (n=4) | 100 | 94-108 |
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||
ng/ml | OD1 | OD2 | Average | Corrected | |||
4000 | 2.660 | 2.634 | 2.647 | 2.563 | |||
2000 | 1.951 | 1.867 | 1.909 | 1.825 | |||
1000 | 1.306 | 1.328 | 1.317 | 1.233 | |||
500 | 0.766 | 0.729 | 0.747 | 0.663 | |||
250 | 0.432 | 0.421 | 0.426 | 0.342 | |||
125 | 0.270 | 0.253 | 0.261 | 0.177 | |||
62.5 | 0.175 | 0.184 | 0.180 | 0.096 | |||
0 | 0.086 | 0.082 | 0.084 |
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CUSABIO's human IL17A ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative determination of IL17A concentrations in serum, plasma, and tissue homogenates. This assay exclusively recognizes human IL17A. The quantitative sandwich ELISA technique of this kit is based on IL17A antibody-IL17A antigen interactions and an HRP colorimetric detection system to detect the levels of IL17A in samples. The intensity of the color is positively proportional to the amount of bound IL17A in the initial step.
IL-17, also known as IL17A, is a key pri-inflammatory cytokine that links T cell activation to neutrophil mobilization and activation. It not only protects the host against extracellular pathogens but also helps to clear intracellular pathogens. IL-17 binding to IL-17RA and IL-17RC activates the ACT1-mediated NF-κB pathway, inducing the production of many inflammatory genes, including TNFα, IL-6, and IL-1. Dysregulated IL-17 production or signaling often leads to unresolved inflammation, resulting in the autoimmune response and tissue destruction. Uncontrolled IL-17 activity is related to different immunopathological conditions, autoimmune disorders, and cancer progression. IL-17 has been shown to promote the formation, growth, and metastasis of various tumors.
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