Human Interleukin 17A (IL-17A/IL-17) ELISA Kit

Code CSB-E12819h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q16552
Lead Time 3-5 working days
Abbreviation IL17A
Target Name interleukin 17A
Alias CTLA8, IL-17, IL-17A, IL17, cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|cytotoxic T-lymphocyte-associated serine esterase 8|interleukin 17 (cytotoxic T-ly
Species Homo sapiens (Human)
Sample Types serum, urine, cell culture supernates, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details This protein is a proinflammatory cytokine produced by activated T cells. This cytokine regulates the activities of NF-kappaB and mitogen-activated protein kinases. This cytokine can stimulate the expression of IL6 and cyclooxygenase-2 (PTGS2/COX-2), as well as enhance the production of nitric oxide (NO). High levels of this cytokine are associated with several chronic inflammatory diseases including rheumatoid arthritis, psoriasis and multiple sclerosis.
HGNC 5981
RGD 2888
MGI 107364
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human IL-17A in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 88  
Range % 81-91  
1:2 Average % 95  
Range % 91-100  
1:4 Average % 98  
Range % 90-107  
1:8 Average % 92  
Range % 82-98  
Recovery
The recovery of human IL-17A spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 91 80-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
400 2.597 2.452 2.525 2.422  
200 2.123 2.086 2.105 2.002  
100 1.446 1.345 1.396 1.293  
50 0.829 0.801 0.815 0.712  
25 0.492 0.472 0.482 0.379  
12.5 0.311 0.300 0.306 0.203  
6.25 0.238 0.219 0.229 0.126  
0 0.106 0.099 0.103    
References
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Function Ligand for IL17RA and IL17RC
Subcellular Location Secreted
Protein Families IL-17 family
Tissue Specificity Restricted to activated memory T-cells.
Database Links

HGNC: 5981

OMIM: 603149

KEGG: hsa:3605

STRING: 9606.ENSP00000344192

UniGene: Hs.41724

Pathway IL-17 signaling pathway
Th17 cell differentiation

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