Human Ly6/PLAUR domain-containing protein 3(LYPD3) ELISA kit

Product Details

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Customer Reviews and Q&A

Target Background

Product Details

Target Name

LY6/PLAUR domain containing 3

Alternative Names

2310061G07Rik ELISA Kit; C4.4A ELISA Kit; GPI-anchored metastasis-associated protein C4.4A homolog ELISA Kit; GPI-anchored metastasis-associated protein homolog ELISA Kit; Ly6/PLAUR domain-containing protein 3 ELISA Kit; Lypd3 ELISA Kit; LYPD3_HUMAN ELISA Kit; Matrigel-induced gene C4 protein ELISA Kit; MIG-C4 ELISA Kit; SHINC3 ELISA Kit

Abbreviation

LYPD3

Uniprot No.

O95274

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates

Detection Range

31.25 pg/mL-2000 pg/mL

Sensitivity

7.81 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Cancer

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human LYPD3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	101
	Range %	94-105
1:2	Average %	88
	Range %	81-93
1:4	Average %	94
	Range %	87-97
1:8	Average %	92
	Range %	84-96

Recovery

The recovery of human LYPD3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-94
EDTA plasma (n=4)	92	85-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.626	2.446	2.536	2.378
1000	2.228	2.113	2.171	2.013
500	1.506	1.488	1.497	1.339
250	0.803	0.785	0.794	0.636
125	0.502	0.491	0.497	0.339
62.5	0.377	0.364	0.371	0.213
31.25	0.246	0.251	0.249	0.091
0	0.156	0.160	0.158

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx

Description

This Human LYPD3 ELISA Kit was designed for the quantitative measurement of Human LYPD3 protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 31.25 pg/mL-2000 pg/mL and the sensitivity is 7.81 pg/mL.

Target Background

Function
(From Uniprot)

Supports cell migration. May be involved in urothelial cell-matrix interactions. May be involved in tumor progression.

Gene References into Functions

While in the healthy liver hepatocytes are C4.4A negative, data show that C4.4A is strongly expressed in hepatocellular carcinoma (HCC) with upregulation at the invasive front and in lung metastasis, indicating that C4.4A apparently contributes to HCC progression. PMID: 29048672
Our results are consistent with previous data in mouse embryogenesis and wound healing. Based on these findings, we conclude that this human TES model provides an excellent surrogate for studies of C4.4A and Haldisin expressions in human stratified epithelia. PMID: 29075641
Expression and crystallographic studies of the D1D2 domains of C4.4A have been reported. PMID: 28777093
LYPD3 has a role in the maintenance of colorectal cancer stem-like cells. PMID: 28238780
Highly expressed C4.4A protein in HER2-positive human breast cancers indicate a good prognosis. PMID: 23918676
overexpression of C4.4A correlates with metastatic potential of gastric cancer and C4.4A could be a novel independent prognostic marker for predicting outcome. PMID: 24935570
expression of the Ly6/uPAR-domain proteins C4.4A and Haldisin in non-invasive and invasive skin lesions PMID: 25414274
Tenascin-C expression was significantly associated with C4.4A expression in clinical esophageal squamous carcinoma samples suggesting that there may be a functional role for the C4.4A to induceTenascin-C in vivo. PMID: 23708783
findings suggest that a tight association between C4.4A and tumor budding may, in part, be due to C4.4A promoting epithelial-mesenchymal transition at the invasive front of colorectal cancer PMID: 22404718
the first explanation for the C4.4A contribution to wound healing and metastasis. PMID: 22431918
data indicate that expression of the C4.4A protein at the invasive front acts as a novel prognostic marker in colorectal cancer, possibly through invasion-related mechanisms PMID: 20825414
hAG-2 and hAG-3, human homologues of genes involved in differentiation, are associated with oestrogen receptor-positive breast tumours and interact with metastasis gene C4.4a and dystroglycan PMID: 12592373
High tumour cell C4.4A expression is associated with shorter survival for non-small cell lung cancer patients. PMID: 17706320
Overexpression of C4.4A is associated with invasion and metastasis of esophageal squamous cell carcinoma PMID: 17849475
we consider C4.4A as a candidate diagnostic marker in colorectal cancer, which possibly can be detected in body fluids PMID: 17912244
cleavage of C4.4A by ADAM10 and ADAM17 contributes to tumor progression PMID: 18979631

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Subcellular Location

Cell membrane; Lipid-anchor, GPI-anchor.

Tissue Specificity

Expressed in placenta, skin and urothelium. Found in suprabasal keratinocytes of chronic wounds. Weak expression is found in esophagus and peripheral blood mononuclear cells. Found in the majority of primary and metastatic transitional cell carcinomas (TC

Database Links

HGNC: 24880

OMIM: 609484

KEGG: hsa:27076

STRING: 9606.ENSP00000244333

UniGene: Hs.631594

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Code	CSB-EL013263HU
Size	96T,5×96T,10×96T
Price	Request a Quote or Start an on-line Chat
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.