Human Peroxiredoxin-1(PRDX1) ELISA kit

Code CSB-EL018653HU
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name peroxiredoxin 1
Alternative Names MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, natural killer-enhancing factor A|proliferation-associated gene A|thioredoxin-dependent peroxide reductase 2
Abbreviation PRDX1
Uniprot No. Q06830
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 3.12 ng/mL-200 ng/mL
Sensitivity 0.78 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cell Biology
Assay Principle quantitative
Measurement Sandwich
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
To assess the linearity of the assay, samples were spiked with high concentrations of human PRDX1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 85  
Range % 81-91  
1:2 Average % 92  
Range % 87-97  
1:4 Average % 96  
Range % 93-99  
1:8 Average % 97  
Range % 91-103  
The recovery of human PRDX1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 90 85-95  
EDTA plasma (n=4) 100 97-103  
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected  
200 2.462 2.497 2.480 2.295  
100 1.726 1.736 1.731 1.546  
50 1.121 1.164 1.143 0.958  
25 0.683 0.698 0.691 0.506  
12.5 0.475 0.498 0.487 0.302  
6.25 0.351 0.367 0.359 0.174  
3.12 0.297 0.287 0.292 0.107  
0 0.186 0.183 0.185    
and FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 7-14 working days
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Target Data

Function Thiol-specific peroxidase that catalyzes the reduction of hydrogen peroxide and organic hydroperoxides to water and alcohols, respectively. Plays a role in cell protection against oxidative stress by detoxifying peroxides and as sensor of hydrogen peroxide-mediated signaling events. Might participate in the signaling cascades of growth factors and tumor necrosis factor-alpha by regulating the intracellular concentrations of H(2)O(2)
Subcellular Location Cytoplasm, Melanosome
Protein Families Peroxiredoxin family, AhpC/Prx1 subfamily
Database Links

HGNC: 9352

OMIM: 176763

KEGG: hsa:5052

STRING: 9606.ENSP00000262746

UniGene: Hs.180909

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