Human Programmed Death Ligand-1(PD-L1/CD274) ELISA Kit

Code CSB-E13644h
See More Details 24T ELISA kits trial application
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9NZQ7
Lead Time 3-5 working days
Abbreviation CD274
Target Name CD274 molecule
Alias B7-H, B7H1, MGC142294, MGC142296, PD-L1, PDCD1L1, PDCD1LG1, PDL1, CD274 antigen|programmed cell death 1 ligand 1
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 15.6 pg/mL-1000 pg/mL
Sensitivity 3.9 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
HGNC 17635
RGD 1566211
MGI 1926446
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human PD-L1/CD274 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 80-101  
1:2 Average % 95  
Range % 91-105  
1:4 Average % 98  
Range % 90-107  
1:8 Average % 95  
Range % 85-100  
Recovery
The recovery of human PD-L1/CD274 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 89-104  
EDTA plasma (n=4) 95 90-107  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
1000 2.274 2.174 2.224 1.965  
500 1.940 1.854 1.897 1.638  
250 1.450 1.355 1.403 1.144  
125 0.927 0.955 0.941 0.682  
62.5 0.570 0.580 0.575 0.316  
31.2 0.422 0.417 0.420 0.161  
15.6 0.352 0.341 0.347 0.088  
0 0.253 0.265 0.259    
References
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Function Involved in the costimulatory signal, essential for T-cell proliferation and production of IL10 and IFNG, in an IL2-dependent and a PDCD1-independent manner. Interaction with PDCD1 inhibits T-cell proliferation and cytokine production.
Subcellular Location Isoform 1: Cell membrane, Single-pass type I membrane protein, SUBCELLULAR LOCATION: Isoform 2: Endomembrane system, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, BTN/MOG family
Tissue Specificity Highly expressed in the heart, skeletal muscle, placenta and lung. Weakly expressed in the thymus, spleen, kidney and liver. Expressed on activated T- and B-cells, dendritic cells, keratinocytes and monocytes.
Database Links

HGNC: 17635

OMIM: 605402

KEGG: hsa:29126

STRING: 9606.ENSP00000370989

UniGene: Hs.521989

Q&A and Customer Reviews

Q&A
Q:

Which antibody is pre-coated onto a microplate? I would appreciate it if you could give me advice.

A:
Thanks for your inquiry.In this kit#CSB-E13644h,Human Programmed Death Ligand-1(PD-L1/CD274) ELISA Kit,2 antibodies are used:
CaptureL:mouse monoclonal antibody;Detection:goat polyclonal antibody
The plate is pre-coated and you can use it directly. Pls let me know if you have any further questions. Thank you.
Customer Reviews

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