CD274, or programmed cell death-ligand 1, alias B7H1, PDCD1L1, PDCD1LG1, PDL1. The protein encoded by CD274 is a type I transmembrane protein with a molecular weight of 40 kDa, and belongs to the CD molecule and B7 family members.
Its mechanism of action involves binding to the PD-1 receptor and transmitting inhibitory signals that attenuate the proliferation of CD8+ T cells and may control the accumulation of antigen-specific T cells in lymph nodes by regulating the Bcl-2 gene. CD274 may play an important role in the suppression of the immune system, for example, in pregnancy, tissue transplantation, autoimmune disorders, and in other diseases such as hepatitis. Normally, the immune system responds to foreign antigens, triggering the proliferation of antigen-specific CD8+ T cells, and PD-1 binds to CD274 to transmit inhibitory signals, thereby suppressing the proliferation of CD8+ T cells.
CD274 expression has prognostic significance in several types of human malignancies and provides tumor cells in the tumor microenvironment by inhibiting cytotoxic T cell activity immune escape.
Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial (Active) (CSB-MP878942HU1)
Validated Data
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.
Activity
Measured by its binding ability in a functional ELISA. Immobilized PD-L1 at 2 μg/ml can bind Anti- PD-L1 mouse monoclonal antibody(CSB-MA878942A1m,antigen from E.coli), the EC50 of human PD-L1 protein is 1.252-1.653 ng/mL.
The purity of CD274 was greater than 90% as determined by SEC-HPLC.
CD274 Recombinant Monoclonal Antibody (CSB-RA878942MA1HU)
Validated Data
IHC image of CSB-RA878942MA1HU diluted at 1:100 and staining in paraffin-embedded human lung cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a Goat anti-Mouse IgG labeled by HRP and visualized using 0.05% DAB.
Overlay Peak curve showing Hela cells surface stained with CSB-RA878942MA1HU (red line) at 1:100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1µg/1*106cells) for 45 min at 4°C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/200 dilution for 35 min at 4°C. Isotype control antibody (green line) was mouse IgG1 (1µg/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.
The following CD274 reagents supplied by CUSABIO are manufactured under a strict quality control system. Multiple applications have been validated and solid technical support is offered.
CD274 Antibodies for Homo sapiens (Human)
Code | Product Name | Species Reactivity | Application |
---|---|---|---|
CSB-PA004911GA01HU | CD274 Antibody | Human,Mouse,Rat | WB, IP, IHC, IF, FC, ELISA |
CSB-PA878942ESR1HU | CD274 Antibody | Human | ELISA, IHC |
CSB-MA878942A0m | PD-L1 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
CSB-MA878942A1m | PD-L1 Monoclonal Antibody | Human | ELISA, WB, IHC, IF, FC |
CSB-RA977797A0HU | CD274 Recombinant Monoclonal Antibody | Human | ELISA, WB, IHC |
CSB-RA878942MA1HU | CD274 Recombinant Monoclonal Antibody | Human | ELISA, IHC, FC |
CD274 Proteins for Homo sapiens (Human)
Code | Product Name | Source |
---|---|---|
CSB-YP878942HU1 CSB-EP878942HU1 CSB-BP878942HU1 CSB-EP878942HU1-B |
Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial | Yeast E.coli Baculovirus In Vivo Biotinylation in E.coli |
CSB-MP878942HU1 | Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial (Active) | Mammalian cell |
CSB-EP878942HU2 | Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial | E.coli |
CSB-EP878942HU1(M) | Recombinant Human Programmed cell death 1 ligand 1 (CD274), partial ( (K136A)) | E.coli |
CSB-YP878942HU1(M)c7 | Recombinant Human Programmed cell death 1 ligand 1 (CD274) (K136A), partial | Yeast |
CD274 Proteins for Mus musculus (Mouse)
Code | Product Name | Source |
---|---|---|
CSB-YP880615MO CSB-EP880615MO CSB-BP880615MO CSB-MP880615MO CSB-EP880615MO-B |
Recombinant Mouse Programmed cell death 1 ligand 1 (Cd274), partial | Yeast E.coli Baculovirus Mammalian cell In Vivo Biotinylation in E.coli |
CD274 ELISA Kit for Homo sapiens (Human)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-E13644h | Human Programmed Death Ligand-1(PD-L1/CD274) ELISA Kit | serum, plasma, tissue homogenates, cell lysates | 3.9 pg/mL |
CD274 ELISA Kit for Mus musculus (Mouse)
Code | Product Name | Sample Types | Sensitivity |
---|---|---|---|
CSB-EL004911MO | Mouse Programmed cell death 1 ligand 1(CD274) ELISA kit | serum, plasma, tissue homogenates | 7.81 pg/mL |