Code | CSB-E12024h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
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Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human PARK7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 87 | ||||
Range % | 80-92 | |||||
1:2 | Average % | 97 | ||||
Range % | 91-105 | |||||
1:4 | Average % | 103 | ||||
Range % | 92-110 | |||||
1:8 | Average % | 93 | ||||
Range % | 86-98 |
The recovery of human PARK7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 95 | 89-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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Parkinson disease (autosomal recessive, early onset) 7 (PARK7), also known as protein DJ-1, is a multifunctional protein that plays key roles in cellular protection against oxidative stress and mitochondrial dysfunction. This protein functions as an antioxidant and molecular chaperone, helping to maintain cellular homeostasis under stress conditions. PARK7 mutations are associated with early-onset Parkinson's disease. This makes it a valuable biomarker for neurodegenerative research and understanding disease mechanisms related to dopaminergic neuron survival.
The Human Protein DJ-1 (PARK7) ELISA kit (CSB-E12024h) provides quantitative measurement of PARK7 in human samples. This sandwich-based assay works with serum, tissue homogenates, and cell lysates. The detection range spans 0.235 ng/mL to 15 ng/mL with sensitivity of 0.058 ng/mL. The assay requires 50-100 μL sample volume and can be completed within 1-5 hours, with detection performed at 450 nm wavelength.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
This ELISA kit has been used in research studies to measure DJ-1 protein levels in biological samples, particularly focusing on its role as a biomarker in various disease contexts. The kit allows researchers to quantify DJ-1 concentrations in serum and culture medium samples across different experimental conditions.
• Biomarker research: Measuring DJ-1 levels in serum samples for disease-related studies and comparative analyses between different patient populations
• Cell culture studies: Measuring DJ-1 protein secretion or release into culture medium from various cell types under experimental conditions
• Inflammatory research: Studying DJ-1 alongside inflammatory cytokines to explore potential relationships between neurodegeneration-associated proteins and inflammatory responses
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