| Code | CSB-PA03724A0Rb |
| Size |
US$299Purchase it in Cusabio online store (only available for customers from the US) |
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| Full Product Name | Rabbit anti-Homo sapiens (Human) PARK7 Polyclonal antibody | ||||||
| Uniprot No. | Q99497 | ||||||
| Target Names | PARK7 | ||||||
| Alternative Names | CAP1 antibody; DJ-1 antibody; DJ1 antibody; DJ1 protein antibody; Epididymis secretory sperm binding protein Li 67p antibody; FLJ27376 antibody; FLJ34360 antibody; FLJ92274 antibody; HEL S 67p antibody; Oncogene DJ1 antibody; OTTHUMP00000001348 antibody; OTTHUMP00000001349 antibody; OTTHUMP00000001350 antibody; OTTHUMP00000001351 antibody; PARK7 antibody; PARK7_HUMAN antibody; Parkinson disease (autosomal recessive, early onset) 7 antibody; Parkinson disease protein 7 antibody; Parkinson protein 7 antibody; Protein DJ-1 antibody; SP22 antibody | ||||||
| Raised in | Rabbit | ||||||
| Species Reactivity | Human, Mouse, Rat | ||||||
| Immunogen | Recombinant Human Protein DJ-1 protein (1-188AA) | ||||||
| Immunogen Species | Homo sapiens (Human) | ||||||
| Conjugate | Non-conjugated The PARK7 Antibody (Product code: CSB-PA03724A0Rb) is Non-conjugated. For PARK7 Antibody with conjugates, please check the following table. |
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| Clonality | Polyclonal | ||||||
| Isotype | IgG | ||||||
| Purification Method | >95%, Protein G purified | ||||||
| Concentration | It differs from different batches. Please contact us to confirm it. | ||||||
| Buffer | Preservative: 0.03% Proclin 300 Constituents: 50% Glycerol, 0.01M PBS, PH 7.4 |
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| Form | Liquid | ||||||
| Tested Applications | ELISA, WB, IHC | ||||||
| Recommended Dilution |
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| Protocols | ELISA Protocol Western Blotting(WB) Protocol Immunohistochemistry (IHC) Protocol |
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| Troubleshooting and FAQs | Antibody FAQs | ||||||
| Storage | Upon receipt, store at -20°C or -80°C. Avoid repeated freeze. | ||||||
| Lead Time | Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time. |
Applications : Western blot analysis
Review: Size fractionation of DJ-1 mitochondrial proteins in plants detected with human DJ-1 antibody. Total mitochondrial proteins and recombinant human Park7 protein were separated by SDS-PAGE. After electrophoresis, proteins were transferred to a membrane and probed with human anti-DJ-1 antibody and developed with TMB substrate. lane 1: size markers (in kDa); lane 2: blank; lane 3: maize mitochondrial protein from leaves grown in light.
By Anonymous
| Function |
Multifunctional protein with controversial molecular function which plays an important role in cell protection against oxidative stress and cell death acting as oxidative stress sensor and redox-sensitive chaperone and protease. It is involved in neuroprotective mechanisms like the stabilization of NFE2L2 and PINK1 proteins, male fertility as a positive regulator of androgen signaling pathway as well as cell growth and transformation through, for instance, the modulation of NF-kappa-B signaling pathway. Has been described as a protein and nucleotide deglycase that catalyzes the deglycation of the Maillard adducts formed between amino groups of proteins or nucleotides and reactive carbonyl groups of glyoxals. But this function is rebuted by other works. As a protein deglycase, repairs methylglyoxal- and glyoxal-glycated proteins, and releases repaired proteins and lactate or glycolate, respectively. Deglycates cysteine, arginine and lysine residues in proteins, and thus reactivates these proteins by reversing glycation by glyoxals. Acts on early glycation intermediates (hemithioacetals and aminocarbinols), preventing the formation of advanced glycation endproducts (AGE) that cause irreversible damage. Also functions as a nucleotide deglycase able to repair glycated guanine in the free nucleotide pool (GTP, GDP, GMP, dGTP) and in DNA and RNA. Is thus involved in a major nucleotide repair system named guanine glycation repair (GG repair), dedicated to reversing methylglyoxal and glyoxal damage via nucleotide sanitization and direct nucleic acid repair. Protects histones from adduction by methylglyoxal, controls the levels of methylglyoxal-derived argininine modifications on chromatin. Able to remove the glycations and restore histone 3, histone glycation disrupts both local and global chromatin architecture by altering histone-DNA interactions as well as histone acetylation and ubiquitination levels. Displays a very low glyoxalase activity that may reflect its deglycase activity. Eliminates hydrogen peroxide and protects cells against hydrogen peroxide-induced cell death. Required for correct mitochondrial morphology and function as well as for autophagy of dysfunctional mitochondria. Plays a role in regulating expression or stability of the mitochondrial uncoupling proteins SLC25A14 and SLC25A27 in dopaminergic neurons of the substantia nigra pars compacta and attenuates the oxidative stress induced by calcium entry into the neurons via L-type channels during pacemaking. Regulates astrocyte inflammatory responses, may modulate lipid rafts-dependent endocytosis in astrocytes and neuronal cells. In pancreatic islets, involved in the maintenance of mitochondrial reactive oxygen species (ROS) levels and glucose homeostasis in an age- and diet dependent manner. Protects pancreatic beta cells from cell death induced by inflammatory and cytotoxic setting. Binds to a number of mRNAs containing multiple copies of GG or CC motifs and partially inhibits their translation but dissociates following oxidative stress. Metal-binding protein able to bind copper as well as toxic mercury ions, enhances the cell protection mechanism against induced metal toxicity. In macrophages, interacts with the NADPH oxidase subunit NCF1 to direct NADPH oxidase-dependent ROS production, and protects against sepsis.
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| Gene References into Functions |
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| Involvement in disease | Parkinson disease 7 (PARK7) |
| Subcellular Location | Cell membrane; Lipid-anchor. Cytoplasm. Nucleus. Membrane raft. Mitochondrion. Endoplasmic reticulum. |
| Protein Families | Peptidase C56 family |
| Tissue Specificity | Highly expressed in pancreas, kidney, skeletal muscle, liver, testis and heart. Detected at slightly lower levels in placenta and brain (at protein level). Detected in astrocytes, Sertoli cells, spermatogonia, spermatids and spermatozoa. Expressed by panc |
| Database Links |
HGNC: 16369 OMIM: 168600 KEGG: hsa:11315 STRING: 9606.ENSP00000340278 UniGene: Hs.419640 |
