Human T-cell surface protein tactile(CD96) ELISA kit

Code CSB-EL004971HU
Size 96T,5×96T,10×96T
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Product Details

Target Name
CD96 molecule
Alternative Names
CD96; T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; CD antigen CD96
Abbreviation
CD96
Uniprot No.
Species
Homo sapiens (Human)
Sample Types
serum, plasma, tissue homogenates, cell lysates
Detection Range
0.156 ng/mL-10 ng/mL
Sensitivity
0.039 ng/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human CD96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 93
Range % 89-97
1:2 Average % 96
Range % 92-100
1:4 Average % 104
Range % 100-112
1:8 Average % 94
Range % 90-100
Recovery
The recovery of human CD96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 84-93
EDTA plasma (n=4) 93 88-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
10 2.045 1.972 2.009 1.836
5 1.502 1.581 1.542 1.369
2.5 1.104 1.054 1.079 0.906
1.25 0.711 0.727 0.719 0.546
0.625 0.510 0.505 0.508 0.335
0.312 0.361 0.349 0.355 0.182
0.156 0.294 0.286 0.290 0.117
0 0.175 0.170 0.173  
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Human CD96 ELISA Kit was designed for the quantitative measurement of Human CD96 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

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Target Background

Function
(From Uniprot)
May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Gene References into Functions
  1. Lower CD96 expression was also observed in human IL-9(+) compared with IFN-gamma(+) T cells PMID: 29531070
  2. Blocking CD96 or TIGIT with mAbs has been shown to improve tumor control in mice. PMID: 27620276
  3. CD96 and CD123 are expressed in bone marrow cells of patients with myelodysplastic syndromes PMID: 26642704
  4. In the present study we analyzed the expression of four cell surface antigens relevant to human hematopoiesis-CD90, CD96, CD117, and CD123-in bone marrow from pediatric acute myeloid leukemia patients and normal control subjects. PMID: 24751333
  5. down-regulation of CD96 is an important aspect of HIV-1 pathogenesis and differential expression is related to cell effector functions and HIV-1 disease course. PMID: 23272144
  6. CD96 could serve as a target structure for effector cell-mediated lysis. PMID: 22879978
  7. The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, relapse and progression. PMID: 21729528
  8. CD96 promotes natural killer (NK) cell adhesion to target cells expressing the poliovirus receptor (PVR), stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. PMID: 15034010
  9. CD96 is a cell surface marker present on many acute myeloid leukemia leukemic stem cells PMID: 17576927
  10. CD96-driven adhesion to CD155 may be crucial in developmental processes PMID: 19056733

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Involvement in disease
C syndrome (CSYN)
Subcellular Location
Membrane; Single-pass type I membrane protein.
Tissue Specificity
Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
Database Links

HGNC: 16892

OMIM: 211750

KEGG: hsa:10225

STRING: 9606.ENSP00000283285

UniGene: Hs.142023

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