Product Details

Description

This Human CD96 ELISA Kit was designed for the quantitative measurement of Human CD96 protein in serum, plasma, tissue homogenates, cell lysates. It is a Sandwich ELISA kit, its detection range is 0.156 ng/mL-10 ng/mL and the sensitivity is 0.039 ng/mL.

Target Name

CD96 molecule

Alternative Names

CD96; T-cell surface protein tactile; Cell surface antigen CD96; T cell-activated increased late expression protein; CD antigen CD96

Abbreviation

CD96

Uniprot No.

P40200

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

0.156 ng/mL-10 ng/mL

Sensitivity

0.039 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Immunology

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CD96 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	89-97
1:2	Average %	96
	Range %	92-100
1:4	Average %	104
	Range %	100-112
1:8	Average %	94
	Range %	90-100

Recovery

The recovery of human CD96 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-93
EDTA plasma (n=4)	93	88-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
10	2.045	1.972	2.009	1.836
5	1.502	1.581	1.542	1.369
2.5	1.104	1.054	1.079	0.906
1.25	0.711	0.727	0.719	0.546
0.625	0.510	0.505	0.508	0.335
0.312	0.361	0.349	0.355	0.182
0.156	0.294	0.286	0.290	0.117
0	0.175	0.170	0.173

ELISA Data Analysis

Watch ELISA data processing video & download Curve Expert if needed

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Target Background

Function (From Uniprot)	May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.
Gene References into Functions	Lower CD96 expression was also observed in human IL-9(+) compared with IFN-gamma(+) T cells PMID: 29531070 Blocking CD96 or TIGIT with mAbs has been shown to improve tumor control in mice. PMID: 27620276 CD96 and CD123 are expressed in bone marrow cells of patients with myelodysplastic syndromes PMID: 26642704 In the present study we analyzed the expression of four cell surface antigens relevant to human hematopoiesis-CD90, CD96, CD117, and CD123-in bone marrow from pediatric acute myeloid leukemia patients and normal control subjects. PMID: 24751333 down-regulation of CD96 is an important aspect of HIV-1 pathogenesis and differential expression is related to cell effector functions and HIV-1 disease course. PMID: 23272144 CD96 could serve as a target structure for effector cell-mediated lysis. PMID: 22879978 The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, relapse and progression. PMID: 21729528 CD96 promotes natural killer (NK) cell adhesion to target cells expressing the poliovirus receptor (PVR), stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. PMID: 15034010 CD96 is a cell surface marker present on many acute myeloid leukemia leukemic stem cells PMID: 17576927 CD96-driven adhesion to CD155 may be crucial in developmental processes PMID: 19056733 Show More Hide All
Involvement in disease	C syndrome (CSYN)
Subcellular Location	Membrane; Single-pass type I membrane protein.
Tissue Specificity	Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.
Database Links	HGNC: 16892 OMIM: 211750 KEGG: hsa:10225 STRING: 9606.ENSP00000283285 UniGene: Hs.142023

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Check Code：

Code	CSB-EL004971HU
Size	96T,5×96T,10×96T
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Human T-cell surface protein tactile(CD96) ELISA kit

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