Product Details

Purity

Greater than 95% as determined by SDS-PAGE.

Endotoxin

Less than 1.0 EU/ug as determined by LAL method.

Activity

Measured by its binding ability in a functional ELISA. Immobilized CD96 at 2 μg/mL can bind anti-CD96 antibody （CSB-RA004971A0HU）, the EC₅₀ is 95.78-127.1 ng/mL.

Target Names

CD96

Uniprot No.

P40200

Alternative Names

(Cell surface antigen CD96)(T cell-activated increased late expression protein)(CD antigen CD96)

Molecular Characterization

Species

Homo sapiens (Human)

Source

Mammalian cell

Expression Region

22-503aa

Target Protein Sequence

VWEKTVNTEENVYATLGSDVNLTCQTQTVGFFVQMQWSKVTNKIDLIAVYHPQYGFYCAYGRPCESLVTFTETPENGSKWTLHLRNMSCSVSGRYECMLVLYPEGIQTKIYNLLIQTHVTADEWNSNHTIEIEINQTLEIPCFQNSSSKISSEFTYAWSVEDNGTQETLISQNHLISNSTLLKDRVKLGTDYRLHLSPVQIFDDGRKFSCHIRVGPNKILRSSTTVKVFAKPEIPVIVENNSTDVLVERRFTCLLKNVFPKANITWFIDGSFLHDEKEGIYITNEERKGKDGFLELKSVLTRVHSNKPAQSDNLTIWCMALSPVPGNKVWNISSEKITFLLGSEISSTDPPLSVTESTLDTQPSPASSVSPARYPATSSVTLVDVSALRPNTTPQPSNSSMTTRGFNYPWTSSGTDTKKSVSRIPSETYSSSPSGAGSTLHDNVFTSTARAFSEVPTTANGSTKTNHVHITGIVVNKPKDGM

Mol. Weight

56.3 kDa

Protein Length

Partial of Isoform 2

Tag Info

C-terminal 10xHis-tagged

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer

Lyophilized from a 0.2 μm filtered 20 mM Tris-HCl, 0.5 M NaCl, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Basically, we can dispatch the products out in 3-7 working days after receiving your orders. Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet & COA

Please contact us to get it.

Description

Recombinant human CD96 protein was expressed with sequence Val22-Met503 of the human CD96 that was fused with a 10xHis-tag at the C-terminus in mammalian cells. Its purity is greater than 95%. Its activity and endotoxin have also been determined.

CD96 is primarily expressed by cells of hematopoietic origin, in specific on T and NK cells. CD96 plays an important role in anti-tumor immune responses and is an ideal candidate for cancer immunotherapy. It has suppressive but also activating NK cell modulating functions and is involved in the regulation of NK cell effector and metastasis. Many studies have revealed the role of the human CD96/human CD155 interaction in target cell elimination by NK cells. Human CD96 upregulation has been found in subpopulations of T-acute lymphoblastic leukemia and AML and has been associated with poor prognosis and increased resistance to chemotherapy. Human CD96 is thus a biomarker for acute myeloid leukemia stem cells (LSC) and is thus a potential target for LSC-specific therapy to treat AML.

Target Background

Function

May be involved in adhesive interactions of activated T and NK cells during the late phase of the immune response. Promotes NK cell-target adhesion by interacting with PVR present on target cells. May function at a time after T and NK cells have penetrated the endothelium using integrins and selectins, when they are actively engaging diseased cells and moving within areas of inflammation.

Gene References into Functions

Lower CD96 expression was also observed in human IL-9(+) compared with IFN-gamma(+) T cells PMID: 29531070
Blocking CD96 or TIGIT with mAbs has been shown to improve tumor control in mice. PMID: 27620276
CD96 and CD123 are expressed in bone marrow cells of patients with myelodysplastic syndromes PMID: 26642704
In the present study we analyzed the expression of four cell surface antigens relevant to human hematopoiesis-CD90, CD96, CD117, and CD123-in bone marrow from pediatric acute myeloid leukemia patients and normal control subjects. PMID: 24751333
down-regulation of CD96 is an important aspect of HIV-1 pathogenesis and differential expression is related to cell effector functions and HIV-1 disease course. PMID: 23272144
CD96 could serve as a target structure for effector cell-mediated lysis. PMID: 22879978
The positive expression of CD96 on bone marrow hematopoietic stem cells in patients with acute leukemia may be associated with primary drug resistance, relapse and progression. PMID: 21729528
CD96 promotes natural killer (NK) cell adhesion to target cells expressing the poliovirus receptor (PVR), stimulates cytotoxicity of activated NK cells, and mediates acquisition of PVR from target cells. PMID: 15034010
CD96 is a cell surface marker present on many acute myeloid leukemia leukemic stem cells PMID: 17576927
CD96-driven adhesion to CD155 may be crucial in developmental processes PMID: 19056733

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Involvement in disease

C syndrome (CSYN)

Subcellular Location

Membrane; Single-pass type I membrane protein.

Tissue Specificity

Expressed on normal T-cell lines and clones, and some transformed T-cells, but no other cultured cell lines tested. It is expressed at very low levels on activated B-cells.

Database Links

HGNC: 16892

OMIM: 211750

KEGG: hsa:10225

STRING: 9606.ENSP00000283285

UniGene: Hs.142023

Code	CSB-MP004971HU1(F2)
Abbreviation	Recombinant Human CD96 protein, partial (Active)
MSDS	MSDS Datasheet
Size	$138
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Image	(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel. Activity Measured by its binding ability in a functional ELISA. Immobilized CD96 at 2 μg/ml can bind anti-CD96 antibody （CSB-RA004971A0HU）, the EC₅₀ is 95.78-127.1 ng/mL.
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Recombinant Human T-cell surface protein tactile (CD96), partial (Active)

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