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Human cystathionine β-synthase(CBS) ELISA Kit

Human cystathionine β-synthase(CBS) ELISA Kit

^Instructions

Code	CSB-E13314h
Size	96T,5×96T,10×96T
Trial Size
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Target Data

Product Details

Target Name

cystathionine-beta-synthase

Alternative Names

AI047524 ELISA Kit; AI303044 ELISA Kit; Beta thionase ELISA Kit; Beta-thionase ELISA Kit; Cbs ELISA Kit; Cbs cystathionine beta-synthase ELISA Kit; CBS_HUMAN ELISA Kit; Cystathionine beta synthase ELISA Kit; Cystathionine beta-synthase ELISA Kit; EC 4.2.1.22 ELISA Kit; HIP 4 ELISA Kit; HIP4 ELISA Kit; Methylcysteine synthase ELISA Kit; MGC18856 ELISA Kit; MGC18895 ELISA Kit; MGC37300 ELISA Kit; OTTHUMP00000109416 ELISA Kit; OTTHUMP00000109418 ELISA Kit; Serine sulfhydrase ELISA Kit

Abbreviation

CBS

Uniprot No.

P35520

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates, cell lysates

Detection Range

125 pg/mL-8000 pg/mL

Sensitivity

31.25 pg/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

Linearity

To assess the linearity of the assay, samples were spiked with high concentrations of human CBS in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	106
	Range %	102-110
1:4	Average %	96
	Range %	90-101
1:8	Average %	88
	Range %	86-96

Recovery

The recovery of human CBS spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	103	98-106
EDTA plasma (n=4)	94	91-97

Typical Data

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
8000	2.144	2.033	2.089	1.978
4000	1.388	1.411	1.400	1.289
2000	0.932	0.989	0.961	0.850
1000	0.655	0.636	0.646	0.535
500	0.445	0.424	0.435	0.324
250	0.266	0.268	0.267	0.156
125	0.212	0.221	0.217	0.106
0	0.110	0.112	0.111
		.

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Lead Time

3-5 working days

Citations

Maternal vitamin D deficiency impact on LCPUFA and pregnancy outcome associated with alterations in the one carbon metabolism AA Nandi,Nutrition Research,2020
Potential biomarkers predicting risk of pulmonary hypertension in congenital heart disease: the role of homocysteine and hydrogen sulfide Sun L et al,Chin Med J (Engl),2014

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Target Data

Function	Hydro-lyase catalyzing the first step of the transsulfuration pathway, where the hydroxyl group of L-serine is displaced by L-homocysteine in a beta-replacement reaction to form L-cystathionine, the precursor of L-cysteine. This catabolic route allows the elimination of L-methionine and the toxic metabolite L-homocysteine
Involvement in disease	Cystathionine beta-synthase deficiency (CBSD)
Subcellular Location	Cytoplasm, Nucleus
Protein Families	Cysteine synthase/cystathionine beta-synthase family
Tissue Specificity	In the adult strongly expressed in liver and pancreas, some expression in heart and brain, weak expression in lung and kidney. In the fetus, expressed in brain, liver and kidney.
Database Links	HGNC: 1550 OMIM: 236200 KEGG: hsa:102724560 UniGene: Hs.533013

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