Code | CSB-E09171h |
Size | 96T,5×96T,10×96T |
Price | Request a Quote or Start an on-line Chat |
Trial Size |
24T ELISA Kit Trial Size (Only USD$150/ kit) * The sample kit cost can be deducted from your subsequent orders of 96T full size kits of the same analyte at 1/5 per kit, until depleted in 6 months. Apply now |
Intra-assay Precision (Precision within an assay): CV%<8% | ||||||
Three samples of known concentration were tested twenty times on one plate to assess. | ||||||
Inter-assay Precision (Precision between assays): CV%<10% | ||||||
Three samples of known concentration were tested in twenty assays to assess. | ||||||
To assess the linearity of the assay, samples were spiked with high concentrations of human CTLA-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. | ||||||
Sample | Serum(n=4) | |||||
1:1 | Average % | 99 | ||||
Range % | 96-102 | |||||
1:2 | Average % | 87 | ||||
Range % | 84-93 | |||||
1:4 | Average % | 111 | ||||
Range % | 107-115 | |||||
1:8 | Average % | 104 | ||||
Range % | 100-108 |
The recovery of human CTLA-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. | ||||||
Sample Type | Average % Recovery | Range | ||||
Serum (n=5) | 85 | 81-89 | ||||
EDTA plasma (n=4) | 92 | 87-98 | ||||
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
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The product CSB-E09171h is a sandwich ELISA kit developed to measure concentrations of human CTLA4 in serum, plasma, or tissue homogenates. This assay uses the sandwich enzyme immunoassay technique in combination with the enzyme-substrate chromogenic reaction to quantify the analyte in the sample. The color develops positively to the amount of CTLA4 in samples. The color intensity is measured at 450 nm via a microplate reader.
CTLA4 is a membrane glycoprotein constitutively expressed on regulatory T (Treg) cells and upregulated on activated T cells. It is involved in the repression of T cell proliferation, cell cycle progression, and cytokine production. CTLA-4 inhibits T cell activation by various suppressive functions including competition with CD28, modulation of the inhibitory function of Treg cells, such as transendocytosis, and the control of adhesion and motility. It is a critical modulator of T cell homeostasis and self-tolerance. Studies have CTLA4 can inhibit cell cycle progression in T-cells by suppressing the generation of cyclin D3 and cyclin-dependent kinases. Human CTLA-4 insufficiency is caused by heterozygous germline mutations in CTLA4 and is characterized by a complex immune dysregulation syndrome.
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