Home
Kit
ELISA Kits
Human glucagon-like peptide-1,GLP-1 ELISA Kit

Human glucagon-like peptide-1,GLP-1 ELISA Kit

Citations (6) Protocol

Code	CSB-E08119h
Size	96T,5×96T,10×96T
Price	Request a Quote
Trial Size	24T ELISA Kit Trial Size (Only USD$150/ kit) * Sample kit cost can be deducted as a $30 credit for each 96-assay kit of the same analyte and brand you subsequently purchase within six months until depleted. More details >> Interested in a trial size? Please leave a message below.
Have Questions?	Leave a Message or Start an on-line Chat

Product Details
Citations (6)
Related Products
Customer Reviews and Q&A

Product Details

Alternative Names

N/A

Abbreviation

GLP-1

Species

Homo sapiens (Human)

Sample Types

serum, plasma, tissue homogenates.

Detection Range

2.5 ng/mL-40 ng/mL

Sensitivity

0.5 ng/mL

Assay Time

1-5h

Sample Volume

50-100ul

Detection Wavelength

450 nm

Research Area

Metabolism

Assay Principle

quantitative

Measurement

Sandwich

Precision

Linearity

Recovery

Typical Data

ELISA Data Analysis

ELISA Standard Curve & Curve Expert software

Troubleshooting
and FAQs

ELISA kit FAQs

Storage

Store at 2-8°C. Please refer to protocol.

Shelf Life

6 months

Lead Time

3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx.

Description

CUSABIO's GLP-1 human ELISA kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human GLP-1 in serum, plasma, or tissue homogenates. This assay uses an antibody specific to human GLP-1 coated on a 96-well plate. GLP-1 present in a sample is bound to the wells by the immobilized antibody after adding the sample into the wells. Biotinylated GLP-1 antibody is added to the wells, forming an antibody-analyte-antibody complex. HRP-conjugated avidin is pipetted to the wells and binds to the complex. A TMB substrate solution is added to the wells and color develops in proportion to the amount of GLP-1 bound. The addition of Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm using a microplate reader.

GLP-1 is a gastrointestinal peptide that is produced by the enteroendocrine L cells and is released in response to meal intake. GLP-1 mainly functions to stimulate insulin secretion and to suppress food intake, gastric emptying, and glucagon secretion, thereby limiting postprandial glucose excursions. It is important for glucose homeostasis. It also inhibits gastrointestinal motility and secretion and thus acts as an enterogastrone and part of the "ileal brake" mechanism. GLP-1 stimulates the proliferation, differentiation, and neogenesis of β cells.

Citations

Effect of Resistance Training with Different Set Structures on Neurotrophic Factors and Obesity-Related Biomarkers in Middle-Aged Korean Women with Obesity HS Bang - Journal of Clinical Medicine,Journal of clinical medicine,2023
Electroacupuncture for functional dyspepsia and the influence on serum Ghrelin, CGRP and GLP-1 levels QIANGLiming.et al,World Journal of Acupuncture-Moxibustion,2018
Acute effect of smoking and smoking abstinence on energy intake and appetite-related hormones blood concentrations Yannakoulia M.et al,Physiol Behav,2017
Purslane (Portulaca oleracea) Seed Consumption And Aerobic Training Improves Biomarkers Associated with Atherosclerosis in Women with Type 2 Diabetes (T2D) F Dehghan.et al,Scientific Reports ,2016
Effect of Coenzyme Q10 on the Incidence of Atrial Fibrillation in Patients With Heart Failure. Zhao Q et al,J Investig Med,2015
Extra virgin olive oil use is associated with improved post-prandial blood glucose and LDL cholesterol in healthy subjects Violi F. et al,Nutr Diabetes,2015

Customer Reviews and Q&A

■ Customer Reviews

There are currently no reviews for this product.

Submit a Review here

■ Q&A

For the CSB-E08119h, Human glucagon-like peptide-1, GLP-1 ELISA Kit, may I ask how we suppose to collect a blood sample and do we need to add DPP-4 inhibitor in EDTA-tube or not?
In Cusabio's instruction, there is no word that explains that we need a DPP-4 inhibitor while collecting blood plasma samples. Do we really not need to add a DPP-4 inhibitor to our sample if we use the Cusabio ELISA kit?

Actually, most CUSABIO ELISA kits are used to detect natural samples. And we usually do not do special treatment such as adding DPP 4 inhibitor in the sample when we detect serum or plasma samples. Though some references state that DPP 4 inhibitor (Dipeptidyl peptidase 4 inhibitors) is one medicine for type 2 diabetes, which could inhibit the inactivation of glucagon-like peptide-1 (GLP-1) and glucose-dependent insulinotropic polypeptide (GIP), and enhance the level of endogenous GLP-1 and GIP. But we did not ever try this kind of operation. Not sure what the result will be.

Promotions

Email: [email protected]
Distributors Worldwide

Service

Phage Display Service

Antibody Service

Protein Service

Gene Synthesis Service

Oligo Synthesis Service

The Collection of Progress of Anti-GPCR Antibody Drugs GIPR: the Crucial Regulator of Glucose and Lipid Metabolism in Diabetes and Obesity! GPCR Drugs Discovery Diabetes: What You Need to Know Before Starting Research? GLP-1R: An Energy Metabolism Regulator of GPCR Family, an Effective Drug Target for Type 2 Diabetes Therapy! CNR1 (CB1): A Crucial Receptor of Endocannabinoid System Offers Potential Treatments for Tumors and Glycolipid Metabolism Disorders! ACVR2A and ACVR2B as Key Targets for Muscle Growth, Weight Loss, PHA in Drug Research！ ELISA Data Analysis How to Do The Pre-experiment before Your ELISA Experiment Four Types of ELISA 11 tips for choosing your right ELISA kit Do you Often Trouble in These Problems of ELISA? Problems and Solutions in ELISA Experiments

Call us

301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)

[email protected]

Address

7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA

Join us with

Subscribe newsletter

Leave a message

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：
If CAPTCHA verification fails, click to refresh the CAPTCHA and try again.

Human glucagon-like peptide-1,GLP-1 ELISA Kit

Product Details

Citations

Related Products

Customer Reviews and Q&A