Macrophage inflammatory protein 2 (MIP-2) is a chemokine that plays an important role in inflammatory responses and immune cell recruitment. This protein belongs to the CXC chemokine family and functions as a potent chemoattractant for neutrophils, promoting their migration to sites of inflammation and tissue injury. Various cell types produce MIP-2, including macrophages, epithelial cells, and fibroblasts in response to inflammatory stimuli. This makes it an important mediator in both acute and chronic inflammatory processes.
The Human macrophage inflammatory protein 2,MIP-2 ELISA Kit (CSB-E07420h) is designed for quantitative measurement of MIP-2 in human samples. This sandwich ELISA can analyze serum, plasma, cell culture supernates, and urine with a detection range of 3.12 pg/mL to 200 pg/mL and sensitivity of 7.81 pg/mL. The assay requires 50-100 μL sample volume, measures at 450 nm wavelength, and can be completed within 1-5 hours.
Application Examples
Note: The following application examples are drawn from a selection of publications citing this product. For additional applications, please refer to the full list of references in the "Citations" section.
Researchers have used this ELISA kit to study inflammatory responses and chemokine signaling pathways in various disease contexts. Studies have applied the kit to quantify CXCL2 levels in biological samples as part of broader cytokine and chemokine profiling efforts. The kit supports research examining immune cell interactions and inflammatory mediator release in both clinical and experimental settings.
• Inflammatory disease research: Used to measure CXCL2 concentrations in serum samples from patients with community-acquired pneumonia compared to healthy controls as part of cytokine profiling studies
• Neuroinflammation studies: Applied to quantify CXCL2 levels in cerebrospinal fluid and cell culture supernatants alongside other inflammatory mediators in research examining central nervous system inflammation
• Cancer immunology research: Applied in chemokine release assays to measure CXCL2 concentrations in supernatants from natural killer cell and bladder cancer cell coculture experiments, supporting studies of immune cell-tumor interactions
• Chemokine profiling: Used as part of comprehensive chemokine panels to assess multiple inflammatory mediators simultaneously in transwell migration assays and cellular interaction studies
