Human macrophage inflammatory protein 2,MIP-2 ELISA Kit

Instructions
Code CSB-E07420h
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name macrophage inflammatory protein 2,MIP-2
Alternative Names C-X-C motif chemokine 2 ELISA Kit; Chemokine (C X C motif) ligand 2 ELISA Kit; Chemokine; CXC motif; ligand 2 ELISA Kit; CINC 2a ELISA Kit; CINC2a ELISA Kit; CINC3 ELISA Kit; CXC chemokine ELISA Kit; CXCL 2 ELISA Kit; Cxcl2 ELISA Kit; CXCL2_HUMAN ELISA Kit; Cytokine-induced neutrophil chemoattractant 3 ELISA Kit; GRO 2 ELISA Kit; GRO b ELISA Kit; GRO protein; beta ELISA Kit; Gro-beta ELISA Kit; GRO-beta(5-73) ELISA Kit; GRO-beta-T ELISA Kit; GRO2 ELISA Kit; GRO2 oncogene ELISA Kit; GROb ELISA Kit; GRObeta ELISA Kit; Growth regulated protein beta ELISA Kit; Growth-regulated protein beta ELISA Kit; GROX ELISA Kit; Hematopoietic synergistic factor ELISA Kit; HSF ELISA Kit; Macrophage inflammatory protein 2 alpha ELISA Kit; Macrophage inflammatory protein 2 ELISA Kit; Macrophage inflammatory protein 2-alpha ELISA Kit; Melanoma growth stimulatory activity beta ELISA Kit; MGSA b ELISA Kit; MGSA beta ELISA Kit; MIP 2 ELISA Kit; MIP 2a ELISA Kit; MIP2 alpha ELISA Kit; MIP2 ELISA Kit; MIP2-alpha ELISA Kit; MIP2A ELISA Kit; MIP2alpha ELISA Kit; SB-251353 ELISA Kit; SCYB 2 ELISA Kit; Scyb ELISA Kit; SCYB2 ELISA Kit; Small inducible cytokine subfamily B; member 2 ELISA Kit
Abbreviation MIP-2
Uniprot No. P19875
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates, urine
Detection Range 3.12 pg/mL-200 pg/mL
Sensitivity 7.81 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human MIP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 92  
Range % 87-100  
1:2 Average % 97  
Range % 91-105  
1:4 Average % 100  
Range % 95-106  
1:8 Average % 93  
Range % 86-98  
Recovery
The recovery of human MIP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 82 80-85  
EDTA plasma (n=4) 89 85-96  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
200 2.352 2.452 2.402 2.276  
100 1.812 1.819 1.816 1.690  
50 1.174 1.273 1.224 1.098  
25 0.703 0.723 0.713 0.587  
12.5 0.425 0.428 0.427 0.301  
6.25 0.301 0.321 0.311 0.185  
3.125 0.201 0.214 0.208 0.082  
0 0.125 0.127 0.126    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Customer Reviews and Q&A

 Customer Reviews

There are currently no reviews for this product.

Submit a Review here

Earn $30 Amazon Card or 20μL/μg CUSABIO Trial Size Antibody. Details of rewards >>

Target Background

Function
(From Uniprot)
Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity.
Gene References into Functions
  1. The studies revealed that, although overall structural and oligomerization features of CXCL3 and CXCL2 are similar, prominent differences were observed in their surface characteristics, thus implicating a functional divergence. PMID: 28928065
  2. Taken together, the data of the present study demonstrated that TcpC can induce MIP2 production, which may contribute to the characteristic histological change associated with pyelonephritis. PMID: 28765918
  3. Functional effects data suggested that recombinant human CXCL2 significantly enhanced the migration, invasion ability of SMMC7721 hepatocellular carcinoma cells, and weakened adhesion ability. PMID: 27117207
  4. Reduced rate of sickle-related complications in Brazilian patients carrying HbF-promoting alleles at the BCL11A and HMIP-2 loci PMID: 26888013
  5. Results identify the CXCL2/MIF-CXCR2 axis as an important mediator in MDSC recruitment and as predictors in bladder cancer. PMID: 27721403
  6. Chronic inflammation contributes to the change of CXCL12 DNA methylation in buccal cells and that DNA methylation profile of CXCL12 promoter plays important role in development and progression of periodontal disease. PMID: 27580404
  7. high GRO-beta expression correlates with poor prognosis and contributes to ovarian cancer tumorigenesis and metastasis. PMID: 26063953
  8. In this review, a genetic variant in CXCL12 is described that is associated with type 2 diabetes mellitus and its complications. PMID: 25085744
  9. We have demonstrated that GRObeta, as an oncogene product, contributed to tumorigenesis and metastasis of HCC PMID: 25801245
  10. Our results demonstrated that resistance to anti-proliferative effects of CXCR2 may also arise from feedback increases in MIP-2 secretion. PMID: 25682075
  11. autophagy is required for Hepatitis B virus-induced NF-kappaB activation and release of IL-6, IL-8, and CXCL2 in Hepatocytes PMID: 25708728
  12. The results link CXCL1 and CXCL2 chemokines with bone marrow adiposity and implicate CXCR2 signaling in promoting effects of marrow fat on progression of skeletal tumors in bone. PMID: 25802102
  13. CXCL2 has antimicrobial activity against E. coli and S. aureus. PMID: 12949249
  14. Simultaneous targeting of hCAP-G2 and MIP-2A is a promising strategy for the development of antitumor drugs as a treatment for intractable tumours. PMID: 24098805
  15. our results demonstrate the diverse mechanisms by which CXCL2 and CXCL3 mediate normal and asthmatic airway smooth muscle cell migration PMID: 23904157
  16. CXCL12 and CXCR4 are related to formation of gastric tumors and lymph node metastasis PMID: 21630055
  17. Ubiquinol decreases monocytic expression and DNA methylation of the pro-inflammatory CXCL2 gene in humans. PMID: 23021568
  18. CXCL2, a WAT-produced chemokine being up-regulated in obesity, stimulates neutrophil adhesion to vis WAT endothelial cells. Activated neutrophils in obesity may influence vis WAT-ECs functions and contribute to WAT inflammation. PMID: 23372021
  19. This is the first report showing the role of CXCL2 in cancer-associated bone destruction. PMID: 22771802
  20. Anti-human ANXA1 antibodies and, to a lesser extent, anti-human ANXA4 antibodies increased MIP-2 or IL-8 production. PMID: 22056994
  21. Data suggest that GRObeta may function as an oncogene product and contribute to tumorigenesis and metastasis of esophageal squamous cell carcinoma. PMID: 21677836
  22. It can be hypothesized that for some targets, such as CXCL1 and CXCL2, additional signaling may be necessary to fully activate the 3'untranslated region-dependent human antigen R (HuR) function in airway epithelium PMID: 21220697
  23. A significantly increased expression of GRO-2, GRO-3, and IL-8 in colon carcinoma as compared to normal tissue, is reported. PMID: 20162422
  24. G-CSF stimulates the expression of the MIP-2 receptor via STAT3-dependent transcriptional activation of Il8rb PMID: 20185584
  25. modulation of the GRO beta concentration in the endometrium by inflammatory mediators may contribute to the normal and pathological processes of human reproduction by regulating the trafficking of neutrophils into the endometrium PMID: 12892904
  26. Neutrophil elastase, MIP-2, and TLR-4 have roles in progression of human sepsis and murine peritonitis PMID: 15614130
  27. CXCL2 tandem repeat promoter polymorphism is associated with susceptibiltiy to severe sepsis in the Spanish population. PMID: 16421598
  28. CXC chemokine CXCL10 and CC chemokine CCL2 serum levels increase with normal aging PMID: 16697212
  29. Inhibition of ERK phosphorylation decreased expression of Grob. PMID: 17466952
  30. data suggest that a tandem repeat polymorphism (AC)n at position -665 in the CXCL2 gene may be an independent predictor of mortality for severe sepsis PMID: 17944017
  31. Decrease of CXCL1 and -2 mediated by Curcumin is involved in the inhibition of metastasis in breast cancer cells. PMID: 17999991
  32. Peripheral neutrophilia and increased serum chemokines (IL-8 and MIP-2) may indicate hepatic injuries in glycogen storage disease type Ia. PMID: 18191274
  33. Resident tissue macrophages are the major source of MIP-2 and KC chemokines; these chemokines are newly synthesized products of signaling through Toll-like receptors. PMID: 18322244
  34. In colon epithelial cells, induction of MIP-2 alpha expression by tumor necrosis factor-alpha was accompanied by a concomitant reduction in miR-192 expression and miR-192 was observed to regulate the expression of MIP-2 alpha. PMID: 18835392
  35. Report gonadotropin-releasing hormone-regulated CXCL2 expression in human placentation. PMID: 19369450

Show More

Hide All

Subcellular Location Secreted.
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

HGNC: 4603

OMIM: 139110

KEGG: hsa:2920

STRING: 9606.ENSP00000427279

UniGene: Hs.75765

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2021 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1