Human secondary lymphoid-tissue chemokine,SLC ELISA Kit

Code CSB-E04521h
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. O00585
Lead Time 3-5 working days
Protein Biological Process 2 chemokine
Abbreviation CCL21
Protein Biological Process 1 Immunity
Target Name chemokine (C-C motif) ligand 21
Alias 6Ckine, CKb9, ECL, MGC34555, SCYA21, SLC, TCA4, Efficient Chemoattractant for Lymphocytes|beta chemokine exodus-2|exodus-2|secondary lymphoid tissue chemokine|small inducible cytokine A21|small indu
Species Homo sapiens (Human)
Protein Biological Process 3 Chemotaxis
Sample Types serum, plasma
Detection Range 78 pg/mL-5000 pg/mL
Sensitivity 20 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Target Details This gene is one of several CC cytokine genes clustered on the p-arm of chromosome 9. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The CC cytokines are proteins characterized by two adjacent cysteines. Similar to other chemokines This protein inhibits hemopoiesis and stimulates chemotaxis. This protein is chemotactic in vitro for thymocytes and activated T cells, but not for B cells, macrophages, or neutrophils. The cytokine encoded by this gene may also play a role in mediating homing of lymphocytes to secondary lymphoid organs. It is a high affinity functional ligand for chemokine receptor 7 (CCR7) that is expressed on T and B lymphocytes and a known receptor for another member of the cytokine family (small inducible cytokine A19).
HGNC 10620
MGI 1349183
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SLC/CCL21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:1 Average % 92
Range % 89-98
1:2 Average % 103
Range % 95-107
1:4 Average % 91
Range % 86-98
1:8 Average % 93
Range % 87-98
Recovery
The recovery of human SLC/CCL21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 97 91-102
EDTA plasma (n=4) 86 82-92
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected
5000 2.983 2.991 2.987 2.844
2500 2.217 2.183 2.200 2.057
1250 1.437 1.489 1.463 1.320
625 0.890 0.883 0.887 0.744
312.5 0.558 0.576 0.567 0.424
156 0.399 0.382 0.391 0.248
78 0.257 0.252 0.255 0.112
0 0.142 0.143 0.143  
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Function Inhibits hemopoiesis and stimulates chemotaxis. Chemotactic in vitro for thymocytes and activated T-cells, but not for B-cells, macrophages, or neutrophils. Shows preferential activity towards naive T-cells. May play a role in mediating homing of lymphocytes to secondary lymphoid organs. Binds to atypical chemokine receptor ACKR4 and mediates the recruitment of beta-arrestin (ARRB1/2) to ACKR4.
Subcellular Location Secreted
Protein Families Intercrine beta (chemokine CC) family
Tissue Specificity Highly expressed in high endothelial venules of lymph nodes, spleen and appendix. Intermediate levels found in small intestine, thyroid gland and trachea. Low level expression in thymus, bone marrow, liver, and pancreas. Also found in tonsil, fetal heart
Database Links

HGNC: 10620

OMIM: 602737

KEGG: hsa:6366

STRING: 9606.ENSP00000259607

UniGene: Hs.57907

Pathway Chemokine signaling pathway
NF-kappa B signaling pathway

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