Human soluble platelet endothelial cell adhesion molecule 1,sPECAM-1 ELISA Kit

Instructions
Code CSB-E08133h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name soluble platelet endothelial cell adhesion molecule 1,sPECAM-1
Alternative Names CD31, FLJ58394, PECAM-1, CD31 antigen|CD31/EndoCAM|PECAM-1, CD31/EndoCAM|adhesion molecule
Abbreviation sPECAM1/sCD31
Uniprot No. P16284
Species Homo sapiens (Human)
Sample Types serum, plasma, cell culture supernates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Others
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SPECAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 95  
Range % 90-100  
1:200 Average % 88  
Range % 84-93  
1:400 Average % 100  
Range % 95-110  
1:800 Average % 90  
Range % 85-97  
Recovery
The recovery of human SPECAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 84 80-90  
EDTA plasma (n=4) 93 89-98  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
2000 2.468 2.324 2.396 2.302  
1000 1.931 1.852 1.892 1.798  
500 1.224 1.176 1.200 1.106  
250 0.568 0.602 0.585 0.491  
125 0.333 0.341 0.337 0.243  
62.5 0.237 0.230 0.234 0.140  
31.25 0.166 0.162 0.164 0.070  
0 0.093 0.094 0.094    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions
Subcellular Location Cell membrane, Single-pass type I membrane protein, Note=Cell surface expression on neutrophils is down-regulated upon fMLP or CXCL8/IL8-mediated stimulation, SUBCELLULAR LOCATION: Isoform Long: Cell membrane, Single-pass type I membrane protein, Membrane raft, Cell junction, Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells, SUBCELLULAR LOCATION: Isoform Delta15: Cell junction
Tissue Specificity Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells (PubMed:18388311, PubMed:21464369). Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level) (PubMed:19342684, PubMed:17580
Database Links

HGNC: 8823

OMIM: 173445

KEGG: hsa:5175

UniGene: Hs.376675

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