Product Details

Uniprot No.

P16284

Target Names

PECAM1

Alternative Names

PECAM1; Platelet endothelial cell adhesion molecule; PECAM-1; EndoCAM; GPIIA'; PECA1; CD antigen CD31

Species Reactivity

Human

Immunogen

A synthesized peptide

Immunogen Species

Homo sapiens (Human)

Conjugate

Non-conjugated

Clonality

Monoclonal

Isotype

Rabbit IgG

Clone No.

7B7

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Rabbit IgG in 10mM phosphate buffered saline , pH 7.4, 150mM sodium chloride, 0.05% BSA, 0.02% sodium azide and 50% glycerol.

Form

Liquid

Tested Applications

ELISA, WB, IHC, FC

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000
IHC	1:50-1:500

Protocols

ELISA Protocol
Western Blotting(WB) Protocol
Immunohistochemistry (IHC) Protocol
Flow Cytometry (FC) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

PECAM-1, commonly designated CD31, serves as a critical adhesion molecule expressed predominantly on endothelial cells, platelets, and various leukocyte subsets. This transmembrane glycoprotein plays essential roles in angiogenesis, vascular integrity, and transendothelial migration of immune cells, making it an indispensable marker for cardiovascular research and studies investigating endothelial biology.

This recombinant monoclonal antibody, clone 7B7, offers the reproducibility and consistency that demanding experimental workflows require. Because the antibody sequence is defined and production occurs in controlled recombinant systems, researchers benefit from lot-to-lot uniformity that traditional hybridoma-derived antibodies cannot guarantee. This consistency proves particularly valuable for longitudinal studies or when standardizing protocols across laboratory sites.

Validation studies confirm reliable performance across multiple applications with human samples. Western blot analysis of THP-1 whole cell lysate demonstrates specific detection at the predicted molecular weight of 125 kDa, with recommended dilutions ranging from 1:500 to 1:5000. Immunohistochemical staining of paraffin-embedded human tonsil tissue reveals clear target localization when used at dilutions between 1:50 and 1:500, providing flexibility for optimization in different tissue contexts. Flow cytometry analysis using Jurkat cells shows distinct positive signal separation from control, enabling quantitative assessment of CD31 expression in cell populations.

The versatility demonstrated across western blotting, immunohistochemistry, and flow cytometry applications positions this antibody as a practical tool for researchers investigating vascular biology, immune cell trafficking, or tumor microenvironment studies where endothelial markers inform experimental interpretation. Whether characterizing endothelial cell populations or examining adhesion molecule expression patterns, this antibody delivers the technical reliability cardiovascular research demands.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Related Products

Target Background

Function

Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient trafficking of PECAM1 to and from the lateral border recycling compartment (LBRC) and is also essential for the LBRC membrane to be targeted around migrating leukocytes. Trans-homophilic interaction may play a role in endothelial cell-cell adhesion via cell junctions. Heterophilic interaction with CD177 plays a role in transendothelial migration of neutrophils. Homophilic ligation of PECAM1 prevents macrophage-mediated phagocytosis of neighboring viable leukocytes by transmitting a detachment signal. Promotes macrophage-mediated phagocytosis of apoptotic leukocytes by tethering them to the phagocytic cells; PECAM1-mediated detachment signal appears to be disabled in apoptotic leukocytes. Modulates bradykinin receptor BDKRB2 activation. Regulates bradykinin- and hyperosmotic shock-induced ERK1/2 activation in endothelial cells. Induces susceptibility to atherosclerosis.; Does not protect against apoptosis.

Gene References into Functions

High CD31 expression is associated with Central Giant Cell Granuloma. PMID: 30139237
TNF-alpha and IL-10 treatment can affect the expression of ICAM-1 and CD31 in human coronary artery endothelial cells. PMID: 29949812
High CD31 expression is associated with early-stage, but not in late-stage, laryngeal squamous cell carcinoma. PMID: 29523110
Adenocarcinomas showed significantly higher staining scores of both VEGF and alphaSMA than squamous cell carcinomas did. In 42 cases of high CD31 score, five-year survival rate (87%) of patients with lung cancer showing mature tumor vessels was significantly better than that (69%) of patients with immature tumor vessels PMID: 29970531
Differences in trafficking of CD31(+) cytotoxic T lymphocytes during acute influenza infection could modulate tolerance and contribute to a dampened adaptive immune response in neonates PMID: 28355204
Cell adhesion assays on wildtype and mutant PECAM-1 further characterized the structural determinants in cell junction and communication. PMID: 27958302
In primary hip OA, angiogenesis may be induced by a combined mechanism: hypoxia-related VEGF-dependent vasculogenesis and endothelial differentiation of the activated pluripotent cells, which are released from the hyperplastic synovial cells layer. An endothelial mesenchymal transition is assumed to be involved in the fibrotic process. PMID: 27704157
upregulation of sVEGFR-1 with concomitant decline of PECAM-1 and sVEGFR-2 levels in preeclampsia compared to normotensive pregnancies, Irrespective of the HIV status PMID: 28609170
Increased expression of PECAM-1, ICAM-3, and VCAM-1 in colonic biopsies from patients with inflammatory bowel disease (IBD) in clinical remission is associated with subsequent flares; this suggests that increases in the expression of these proteins may be early events that lead to flares in patients with IBD. PMID: 27552332
PECAM-1 gene polymorphisms are associated with Kawasaki disease with and without coronary artery lesions in Chinese children. PMID: 28512385
Sirt1 expression is associated with CD31 expression in endothelial progenitor cells from patients with chronic obstructive pulmonary disease. PMID: 27784320
we found a significant cumulative contribution of the genetic heterogeneity of glycoproteins Ia and IIIa and platelet-endothelial cell adhesion molecule-1 and P-Selectin genes in the risk of recurrent IVF-ET failures. The coexistence of these SNPs was associated with increased IVF-ET failure risk and the more polymorphic alleles or genotypes were present the higher the risk of IVF-ET failure, especially for younger women PMID: 28388872
Dimer conformation of soluble PECAM-1 PMID: 27270333
RrgA, binds both polymeric immunoglobulin receptor (pIgR) and PECAM-1, whereas the choline binding protein PspC binds, but to a lower extent, only pIgR PMID: 28515075
this study shows a significant role for CD31 during T cell development PMID: 28159903
Cells in high glucose for 7 days showed a significant decrease in mRNA expression of CD31 and VE-cadherin, and a significant increase in that of alpha-SMA and collagen I. PMID: 28347704
Patients who had optic neuritis that progressed to multiple sclerosis had a decrease in serum PECAM-1 levels. PMID: 27806869
Platelet endothelial cell adhesion molecule (PECAM-1) is expressed in endothelial cells (ECs), platelets, and leukocytes, regulating the interaction between those cells. PMID: 27079772
Data indicate no association of maternal or fetal ITGA2 C807T SNP, ITGB3 T1565C SNP, PECAM1 CTG - GTG and SELP A/C polymorphisms with fetal growth restriction (FGR). PMID: 28358707
these data suggest that a sialic acid-containing glycan emanating from Asn-25 reinforces dynamic endothelial cell-cell interactions by stabilizing the PECAM-1 homophilic binding interface. PMID: 27793989
Decreased FoxP3 expression in CD31(+) Tr cells. PMID: 27997991
These results suggested that PECAM-1 could mediate platelet adhesion to endothelial cells under shear stress. Platelets binding to endothelial cells interfered with endothelial cell mechanotransduction through PECAM-1, affecting endothelial cell inflammatory responses towards pathological shear flow. PMID: 28013181
Immunohistochemical expression of CD31 and vascular endothelial growth factor (VEGF) were assessed in parallel. PMID: 27270504
CD31 is expressed in mycosis fungoides (MF) skin biopsies, which provides new evidence for the role of angiogenesis in the progression of MF PMID: 27630298
The Leu125Val polymorphism of PECAM-1 and the level of soluble PECAM-1 are not associated with diabetic nephropathy in Caucasians with type 2 diabetes mellitus. PMID: 28116228
We provide the first report that pro-angiogenic genes PECAM1, PTGS1, FGD5, and MCAM may play a vital role in pathological dermal angiogenesis disorders of psoriasis. PMID: 26748901
The PECAM-1 functions as an adhesive stress-response protein to both maintain endothelial cell junctional integrity and speed restoration of the vascular permeability barrier following inflammatory or thrombotic challenge. PMID: 27055047
These studies indicate a role for PECAM-1 in enhancing the inhibitory functions of TGF-beta in T cells PMID: 26956486
PECAM1 plays an important role in the formation of tight junction complex. PMID: 26607202
The most significant associations were detected for PECAM1*V/V + DDAH1*C (OR = 4.17 CI 1.56-11.15 Pperm = 0.005) PMID: 26662939
soluble CD38 (sCD38) in seminal plasma increases the capacitation of sperm via specific interactions between sCD38 and the CD31 on the sperm. PMID: 26407101
expression levels of CD31/ PECAM1 are deregulated in human glioblastoma multiforme tissue specimens; correlation among CD31/PECAM1 and HIF-1alpha and N-cadherin and ADAM-10, two other markers of aggressiveness in the same tumors PMID: 26376118
Radiation-induced stress conditions induce a transient accumulation of granulocytes within the liver by down-regulation/absence of PECAM-1. PMID: 26177067
Suggest that CD31 expression correlates with prognosis in gastrointestinal stromal tumors. PMID: 26078569
PECAM-1 125C/G polymorphism is associated with deep vein thrombosis. PMID: 25846278
Heterogeneity was found in the endothelial cells: their shape, the expression of adhesion molecules(ICAM-1, VCAM-1, and PECAM ), and the adhesion of lymphocytes and monocytes to them changed during the progression of the atherosclerotic process. PMID: 26841644
PECAM1+ melanoma cells form vascular channels PMID: 25335460
Low shear stress can induce inflammatory response via PECAM-1/PARP-1/HMGB1 pathway. PMID: 25793984
Nck promoted oxidative stress-induced activation of NF-kappaB by coupling the tyrosine phosphorylation of PECAM-1 (platelet endothelial cell adhesion molecule-1) to the activation of p21-activated kinase PMID: 25714462
PECAM-1 has a role in mediating the profibrotic and prometastasic environment caused by ethanol in endothelial cells PMID: 24734240
Report correlation between mast cell tryptase and CD31 expression in odontogenic tumors. PMID: 26247531
there are associations between various PECAM-1 polymorphisms in rheumatoid arthritis and systemic lupus erythematosus patients, and PECAM-1 polymorphisms in SLE are protective against atherosclerotic complications PMID: 25201689
preeclampsia does not significantly affect vascular growth or the expression of endothelial junction proteins in human placentas PMID: 25362142
C-CD31 have impaired angiogenic potential and the number of circulating CD31(+) cells were correlated with cardiovascular disease risk PMID: 25267411
demonstrate a functional link between HO-1 gene expression and PECAM-1 in endothelial cells, which might play a critical role in the regulation of inflammation PMID: 24500083
The positive correlation is established between content of polymorphic nuclear monocytes and level of expression of molecules of LFA-1, ICAM-1, LFA-3, and PECAM-1. PMID: 25884075
High CD31 expression associated significantly with better survival and VEGFR3 had no association with survival. Both higher tumor grade and stage were associated with a decreased survival time PMID: 25667475
Suggest that cell confluence and the type of flow are critical independent factors in the induction of TF and PECAM-1 phosphorylation in endothelial cells exposed to disturbed pulsatile flow and chemical stimuli. PMID: 24342062
The finding that the adhesive properties of PECAM-1 are regulatable suggests novel approaches for controlling endothelial cell migration and barrier function in a variety of vascular permeability disorders. PMID: 24936065
for the cytological diagnosis of angiosarcomas, ERG and CD31 are more sensitive vascular markers than CD34 PMID: 25352641

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Subcellular Location

Cell membrane; Single-pass type I membrane protein.; [Isoform Long]: Cell membrane; Single-pass type I membrane protein. Membrane raft. Cell junction.; [Isoform Delta15]: Cell junction. Note=Localizes to the lateral border recycling compartment (LBRC) and recycles from the LBRC to the junction in resting endothelial cells.

Tissue Specificity

Expressed on platelets and leukocytes and is primarily concentrated at the borders between endothelial cells. Expressed in human umbilical vein endothelial cells (HUVECs) (at protein level). Expressed on neutrophils (at protein level). Isoform Long predom

Database Links

HGNC: 8823

OMIM: 173445

KEGG: hsa:5175

UniGene: Hs.376675

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Code	CSB-RA017767A0HU
Size	US$210
	Order now
Image	Western Blot Positive WB detected in THP-1 whole cell lysate All lanes CD31 antibody at 0.95μg/ml Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 125 KDa Observed band size: 125 KDa IHC image of CSB-RA017767A0HU diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4℃ overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system. Overlay histogram showing Jurkat cells stained with CSB-RA017767A0HU (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min.The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4℃.The secondary antibody used was FITC goat anti-rabbit IgG (H+L) at 1/200 dilution for 1 h at 4℃. Control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.
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CD31 Recombinant Monoclonal Antibody

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Target Background