Human suppressors of cytokine signaling 3,SOCS-3 ELISA Kit

Instructions
Code CSB-E11637h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
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Product Details

Target Name suppressor of cytokine signaling 3
Alternative Names ATOD4 ELISA Kit; CIS 3 ELISA Kit; CIS-3 ELISA Kit; CIS3 ELISA Kit; Cish3 ELISA Kit; Cytokine induced SH2 protein 3 ELISA Kit; Cytokine-inducible SH2 protein 3 ELISA Kit; E2a Pbx1 target gene in fibroblasts 10 ELISA Kit; EF 10 ELISA Kit; MGC71791 ELISA Kit; SOCS 3 ELISA Kit; SOCS-3 ELISA Kit; Socs3 ELISA Kit; SOCS3_HUMAN ELISA Kit; SSI 3 ELISA Kit; SSI-3 ELISA Kit; SSI3 ELISA Kit; STAT induced STAT inhibitor 3 ELISA Kit; STAT-induced STAT inhibitor 3 ELISA Kit; Suppressor of cytokine signaling 3 ELISA Kit
Abbreviation SOCS3
Uniprot No. O14543
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 47 pg/mL-3000 pg/mL
Sensitivity 11.75 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human SOCS-3 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 95  
Range % 88-99  
1:2 Average % 94  
Range % 82-102  
1:4 Average % 91  
Range % 84-105  
1:8 Average % 96  
Range % 90-102  
Recovery
The recovery of human SOCS-3 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 96 83-110  
EDTA plasma (n=4) 97 86-104  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
3000 2.069 2.105 2.087 2.002  
1500 1.486 1.517 1.502 1.417  
750 1.108 1.034 1.071 0.986  
375 0.591 0.603 0.597 0.512  
187.5 0.396 0.408 0.402 0.317  
93.75 0.286 0.274 0.280 0.195  
46.88 0.210 0.203 0.207 0.122  
0 0.083 0.087 0.085    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Target Data

Function SOCS family proteins form part of a classical negative feedback system that regulates cytokine signal transduction. SOCS3 is involved in negative regulation of cytokines that signal through the JAK/STAT pathway. Inhibits cytokine signal transduction by binding to tyrosine kinase receptors including gp130, LIF, erythropoietin, insulin, IL12, GCSF and leptin receptors. Binding to JAK2 inhibits its kinase activity. Suppresses fetal liver erythropoiesis. Regulates onset and maintenance of allergic responses mediated by T-helper type 2 cells. Regulates IL-6 signaling in vivo (By similarity). Probable substrate recognition component of a SCF-like ECS (Elongin BC-CUL2/5-SOCS-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Seems to recognize IL6ST (By similarity).
Gene References into Functions
  1. Downregulation of miR-340 inhibited GC cell proliferation, arrested cell cycle, and facilitated apoptosis through upregulating SOCS3 expression to suppress JAK-STAT3 signaling pathway. PMID: 29658372
  2. Low SOCS3 expression is associated with higher grade breast cancer. PMID: 30453988
  3. Low SOCS3 expression is associated with invasion and lymph node metastasis in gastric cancer. PMID: 29970682
  4. Results show the CAT haplotype in SOCS3 was associated with metabolic syndrome (MetS) in Mexican Mestizos, and type 2 diabetes in Mexican Amerindians. PMID: 30176313
  5. SOCS3 overexpression decreased JAK-STAT3 signaling pathway activity, declined Bcl-2 expression, inhibited cell proliferation, elevated cell apoptosis, and enhanced Adriamycin sensitivity in T24 cells PMID: 28742207
  6. SOCS3 DNA methylation is associated with body weight and moderates the effect of cumulative stress on obesity. PMID: 27826092
  7. polymorphisms associated with risk of head and neck squamous cancer PMID: 27977878
  8. SOCS3 was targeted by miR30a- 5p in allergic rhinitis PMID: 29471302
  9. Cellular viability was significantly decreased, whereas IL6 and TNFalpha levels were significantly increased, following High Glucose stimulation of A549 cells. In addition, the protein levels of SOCS3, pJAK2 and pSTAT3 were significantly increased in High Glucosetreated cells. PMID: 28713982
  10. this study shows that Nrf2 activation induces lipocyte phenotype in hepatic stellate cells via enhancing SOCS3-dependent feedback inhibition on JAK2/STAT3 cascade PMID: 28601022
  11. Our study showed that the expressions of HER2, STAT3, and SOCS3 are associated with the progression of ovarian cancer (OC), and higher expressions of HER2 and STAT3 and lower expression of SOCS3 predict poor prognosis of OC. PMID: 28448787
  12. Authors found that SOCS3 and SOCS1 expression was reduced in vivo, in tumor lesions of BCC and SCC, as compared to other skin inflammatory conditions such as psoriasis, despite the high number of IL-22-secreting TILs. PMID: 28445952
  13. the presence of SOCS3 methylation is a marker to predict treatment response and prognosis in HCC patients receiving TACE therapy. PMID: 28404963
  14. anti-miR-203 suppresses breast cancer cell proliferation in vitro by targeting SOCS3 [review] PMID: 27517632
  15. miR-2909 could play a vital role in prostate carcinogenesis through modulation of ISGylation system and TGFbeta signalling via STAT1/SOCS3. PMID: 28622443
  16. Data show that miR-222-3p targeted SOCS3 genes and down-regulation of SOCS3 correlated with an increased expression of STAT3 activation. PMID: 27172798
  17. increased SOCS3 in combined IL-33 heterozygotes and minor allele homozygotes may be relevant for hay fever development PMID: 27171815
  18. In summary, this study has shown that LIF is implicated in the HG-mediated inhibition of osteoblast differentiation, via promoting STAT3/SOCS3 signaling. This study may provide insights into the signal pathway of HG-induced bone loss or delayed injured joint healing. PMID: 28064096
  19. Data suggest that SOCS3 is an important signaling protein in CLL, and Hsp90 inhibitors represent an approach to target transcriptional repression in B cell lymphoproliferative disorders. PMID: 27107422
  20. 6-Hydroxy-3-O-methyl-kaempferol 6-O-glucopyranoside potentiated the inhibitory effect of IFN-alpha on hepatocellular carcinoma cell proliferation through activation of the JAK/STAT signaling pathway by inhibiting SOCS3 expression. PMID: 29031523
  21. SOCS3 was down-regulated in CML cell lines and most of BMNCs from CML patients, and the expression level of SOCS3 was associated with the inhibition of cell proliferation and drug resistance of CML cells. PMID: 27516205
  22. SOCS3 is involved in suppression of Helicobacter pylori induced STAT3 phosphorylation by docosahexaenoic acid pretreatment. PMID: 27079734
  23. The increased miR-203 expression is associated with the reduced anti-inflammatory mediator SOCS-3 expression and the increased endothelial adhesion molecule expression in maternal vessel endothelium in preeclampsia. PMID: 27753461
  24. Our results show, for the first time, that SOCS-3 regulates leptin-induced responses in cartilage PMID: 27716333
  25. In M1-activated human monocyte-derived macrophages, SOCS3 silencing decreased expression of proinflammatory markers and increased M2 macrophage markers. SOCS3 knockdown radically affects the temporal dynamics of M1 macrophages' particle engulfment through increased PI3K and Ras-related C3 botulinum toxin substrate 1 (Rac1) activity. SOCS3 drives macrophage inflammatory responses and modulates key phagocytosis signaling. PMID: 27106674
  26. The results of this study demonstrated that HBx of hepatitis B virus impairs interferon signaling via increased expression of SOCS3 and PP2A. PMID: 27459003
  27. Overexpression of SOCS3 inhibited proliferation, migration, invasion and tumorigenic ability of colorectal cancer cells while increased cell apoptosis. Reduced expression of SOCS3 promoted the growth and metastasis of colorectal cancer. PMID: 28603075
  28. SOCS3, which is a critical negative regulator of cytokine response to tuberculosis infection and its nearby lncRNA XLOC_012582, were highly expressed in active tuberculosis B cells. PMID: 28125665
  29. Data show that the suppressor of cytokine signaling-3 (SOCS3) promoter hypermethylation was associated with relatively low mRNA expression in tumor tissues. PMID: 28179578
  30. Data show that ELOVL7, SOCS3, ACSL4 and CLU were upregulated while PRKAR1A and ABCG1 were downregulated in the phlegm-dampness group. PMID: 27928700
  31. There are pronounced changes in the expression of SOCS3 only in Non-allergic Asthma. PMID: 28378729
  32. epigenetic signatures at AQP3 and SOCS3 engage in low-grade inflammation across different tissues, possible via JAK/STAT mediated pathways PMID: 27824951
  33. SHP2, SOCS3 and PIAS3 levels are reduced in medulloblastomas in vivo and in vitro, of which PIAS3 downregulation is more reversely correlated with STAT3 activation. In resveratrol-suppressed medulloblastoma cells with STAT3 downregulation and decreased incidence of STAT3 nuclear translocation, PIAS3 is upregulated, the SHP2 level remains unchanged and SOCS3 is downregulated. PMID: 28035977
  34. SOCS3 methylation status was associated with multiple metabolic syndrome traits, including central obesity, fat depots, insulin responsiveness, and plasma lipids. PMID: 27564309
  35. reduced type I interferon production in obesity is caused by SOCS3 overexpression as well as tolerance induced by leptin PMID: 27704310
  36. Data suggest that single nucleotide polymorphisms (SNPs) in suppressor of cytokine signaling 3 (SOCS3) may play a role in the pathogenesis of metabolic syndrome (MS). PMID: 27611604
  37. that siRNA-mediated SOCS3 knock-down in human osteoarthritic chondrocytes increased production of IL-1beta-induced prostaglandin E2 PMID: 28296552
  38. SOCS3 promoter was methylated in H2228 cells as well as in three cases of seven EML4-ALK-positive lung cancer tissues. PMID: 27666544
  39. Data suggest that microRNA miR19a3p plays an important role in beta cell function, and that the miR-19a-3p/suppressor of cytokine signaling 3 (SOCS3) axis may become a potential therapeutic target for diabetes. PMID: 27666763
  40. Significantly higher levels of suppressor of cytokine signalling proteins SOCS3 and SOCS4 in non-healing chronic wounds compared to the healing/healed chronic wounds were observed at the transcript level. PMID: 27635428
  41. The rs115785973 could be regulated by miR-4308 which caused an up-regulation ofsuppressor of cytokine signaling 3 in patients with GA and AA genotype. PMID: 27160093
  42. SOCS3 along with STAT3 participates in regulatory mechanisms in ccRCC. PMID: 26797799
  43. None of the clinicopathological parameters were associated with the expressions of FIH-1 and SOCS3 at mRNA level. PMID: 26749281
  44. Micronome revealed miR-19a/b as key regulator of SOCS3 during cancer related inflammation of oral squamous cell carcinoma. PMID: 27581787
  45. IGFBP2 and SOCS3 were up-regulated in Kashin-Beck disease chondrocytes and decreased with increasing selenium concentrations. IGFBP2 expressed highest in the middle zone of KBD cartilage, SOCS3 expressed higher in the middle and deep zone. IGFBP2 and SOCS3 may be the biomarkers for KBD diagnosis and evaluating the effect of selenium supplement. PMID: 27099071
  46. Results show that IFN-alpha antagonizes STAT3 and SOCS3 signaling triggered by hepatitis C virus (HCV )and that STAT3 regulation correlates inversely with SOCS3 induction by IFN-alpha, which may be important in better understanding the complex interplay between IFN-alpha and signal molecules during HCV infection. PMID: 26945996
  47. data show that HBV-induced mitochondrial ROS production represses SOCS3 expression through Snail-mediated epigenetic silencing, leading to the sustained activation of IL-6/STAT3 pathway and ultimately contributing to hepatocarcinogenesis. PMID: 26794444
  48. demonstrate an epigenetic mechanism of SOCS3 up-regulation in steroid resistant children with the nephrotic syndrome. The assessment of methylation/unmethylation of SOCS3 promoter might be an early marker for steroid responsiveness in NS patients PMID: 26828667
  49. Treatment of isolinderalactone increased the protein level of SCOS3, decreased phosphorylation of the STAT3 and suppressed expression of the down-stream genes of the X-linked inhibitor of apoptosis protein PMID: 26707189
  50. Dysregulation of SOCS3 functions can cause a variety of diseases, including allergy, autoimmune diseases, inflammation and cancer. (Review) PMID: 26429311
  51. induction of SOCS3 may be a novel mechanism by which FXR exerts its anti-hepatocellular carcinoma effects PMID: 26416445
  52. H. pylori infection induced hepatic insulin resistance by the c-Jun/miR-203/SOCS3 signaling pathway and provide possible implications with regard to resolving insulin resistance. PMID: 25689935
  53. A significant relevance involved in SOCS3 gene polymorphisms and infantile asthma development based on a Chinese Han population. PMID: 26464723
  54. In hepatocellular carcinoma tumor tissues, SOCS3 showed an increased methylation frequency and intensity compared with that in the adjacent non-tumor tissues. PMID: 26393582
  55. Data suggest that PCSK9 is involved in regulation of lipid metabolism in hepatocytes by proinflammatory cytokine TNFalpha; induction of lipogenesis and insulin resistance in liver (as seen in obesity) involves up-regulation of both PCSK9 and SOCS3. PMID: 26668321
  56. Epigenetic inhibition of SOCS3 is regulated by miR1264 which specifically targets DNMT1 transcript. PMID: 26047583
  57. Decreased SOCS3 expression is associated with cholangiocarcinoma. PMID: 26485275
  58. SOCS3 down-expression induces lineage commitment towards erythroid progenitor cell fate by activation of erythroid-specific gene in HSCs PMID: 26252772
  59. Suggest racial/ethnic- and/or sex-specific association between the SOCS3 A+930-->G (rs4969168) SNP and serum APOA1/LDL cholesterol levels in some Chinese populations. PMID: 26261631
  60. Increased SOCS3 expression in PBMCs and T cells from patients with ankylosing spondylitis correlates with patient's functional ability, acute-phase reactants and serum pro-inflammatory cytokines. PMID: 26812031
  61. This study showed that there was significantly increased levels of SOCS-2 mRNA in elderly and Alzheimer's disease brain. PMID: 25286386
  62. Lower expression of SOCS3 is associated with response to radiation in cervical cancer. PMID: 25849377
  63. Enhanced expression of suppresser of cytokine signaling 3 inhibits the IL-6-induced epithelial-to-mesenchymal transition and cholangiocarcinoma cell metastasis. PMID: 25744243
  64. there is a regulatory pathway consisting of miR-30, SOCS3, and Jak/STAT3 in GSCs, and targeting this pathway may be a promising strategy to treat glioma. PMID: 25840690
  65. SOCS-3 may have a role in dyslipidemia in China in the Xinjiang Uygur population PMID: 25730073
  66. Decreased SOCS3 and IL-35 plasma levels and increased Th1/Th2 cytokine ratios in RPL cases pointed out the supression of anti-inflammatory process and this suppression might play an important role in the pathogenesis of idiopathic RPL. PMID: 24762139
  67. Our findings suggest that the rs4969170A>G polymorphism of SOCS3 gene may be used as a prognostic predictor for HCC patients who underwent surgical treatment. PMID: 26447993
  68. SOCS3 negatively regulates proliferation and angiogenesis of NCI-H446 cells and that HIF-1alpha is required in this process. The results also suggested a suppressive role of SOCS3 in Akt signaling, but not STAT3 signaling to block HIF-1alpha expression PMID: 25695729
  69. crucial requirements for IL-31 signaling and its counter-regulation by SOCS3 PMID: 26306032
  70. Varicella-zoster virus infection induces SOCS3 expression, resulting in modulation of type I interferon signaling and viral replication. PMID: 26072679
  71. The role of the SOCS3 protein in the Jak/stat3 pathway is to mediate different mechanisms for preterm and term labor processes in the placenta. PMID: 26178755
  72. SOCS3 is an inducible host factor that regulates virus egress during Ebola virus infection. PMID: 26246577
  73. Reduced SOCS3 expression in eosinophils, lead to an inhibition of GATA-3 and FoxP3, impaired eosinophil migration, adhesion and degranulation. PMID: 25764157
  74. No correlations were reported between adipokine profile, HOMA-IR values and hepatic expression of the SOCS-3 gene in chronic hepatitis C. PMID: 25132761
  75. SOCS3 overexpression is associated with early relapse of mucosal inflammation in in ulcerative colitis. PMID: 25545374
  76. the present study demonstrated that advanced grade of urothelial bladder carcinoma was significantly associated with lowered mRNA expression of SOC-3 as well as elevated urinary levels of proinflammatory cytokines and CK-18. PMID: 25640370
  77. Data indicate that suppressor of cytokine signaling 3 (SOCS3) knockdown markedly increases the abundance of TANK-binding kinase 1 (TBK1). PMID: 25939384
  78. Data suggest that SOCS3 plays critical negative role in regulation of expression of TRAIL (TNF-related apoptosis-inducing ligand) in endoplasmic reticulum stress in macrophages via Jun N-terminal kinase/AP-1 transcription factor signaling. PMID: 25827060
  79. SOCS3 and STAT3 are the major controllers of the outcome of infection with Mycobacterium tuberculosis. (Review) PMID: 25458989
  80. Results so that AA genotype carriers had significantly higher levels of SOCS3 RNA and have greater susceptibility to GO PMID: 25770161
  81. Reg3A overexpression promoted cell growth in pancreatic cancer. SOCS3 is a key target in cancer by inhibiting cell growth and inducing apoptosis. SOCS3 negatively regulated Reg3A-mediated cell growth in pancreatic cancer. PMID: 24996521
  82. found SOCS3 gene polymorphisms are not associated with occurrence of adolescent idiopathic scoliosis (AIS) but the polymorphism(rs4969168)is associated with abnormal growth pattern of AIS, indicating SOCS3 gene might be a disease-modifying gene of AIS PMID: 25034082
  83. Loss of CAST in angiogenic ECs facilitates mu-calpain-induced SOCS3 degradation, which amplifies pathological angiogenesis through interleukin-6/STAT3/VEGF-C axis. PMID: 25648699
  84. Low expression of SOCS-3 is associated with gastric cancer. PMID: 25261987
  85. We investigated the role of SOCS3 in p53- and PTEN-mediated activation of inflammatory feedback loop and malignant transformation PMID: 24531711
  86. Data demonstrate that the serum levels of SOCS3 and STAT3 were increased in childhood atopic dermatitis (AD) compared with controls. PMID: 24931262
  87. Inhibition of SOCS3 expression promoted the proliferation, migration, and invasion of HepG2 cells. PMID: 24476004
  88. This study suggest that SOCS3 regulates IL-6 signaling as an antagonist in CSF. PMID: 25125049
  89. Down- regulation of both SOCS-1 and SOCS-3 genes in resistant cells. PMID: 24969884
  90. Increased expression of SOCS3 may serve to perpetuate "inflammation" by promoting TNF-alpha production and limiting epithelial repair in response to commensal microflora. PMID: 25377316
  91. Variations in genes AGRP, CPE, GHRL, GLP1R, HTR2A, NPY1R, NPY5R, SOCS3 and STAT3 showed modest associations with BMI in European Americans. PMID: 23900445
  92. IL-8 and CXCR2 expression was distinctly uncommon as opposed to IL-6, VEGF and SOCS-3, which were detected in the vast majority of cases. PMID: 24883303
  93. SOCS3 promoter hypermethylation status has a favorable prognostic value in GBM patients because of whole genome methylation status. Particularly, the hypermethylation of the SOCS3 promoter indicates positive G-CIMP status. PMID: 24633048
  94. Elevated SOCS-3 overexpression is associated with tumor aggressiveness in renal cell carcinoma. PMID: 24593195
  95. These results demonstrate a role for SOCS3 in regulating proinflammatory TNF-alpha signal transduction and reveal a novel immune-modulatory mechanism by which HCV suppresses inflammatory responses PMID: 24659790
  96. Results show that the cytokine signaling-3 (SOCS3) expression was significantly elevated in both the liver tissue and peripheral blood mononuclear cells (PBMCs) from patients with HBV-induced acute-on-chronic liver failure (HBV-ACLF). PMID: 24939297
  97. a reduction in function, rather than a reduction in expression of SOCS3 accounts for the unregulated expression of IL-17 in SS, and may play a crucial role in aetiopathogenesis. PMID: 24827536
  98. Cannabinoids induce autophagy in a model of intestinal epithelium, an effect that leads to the reduction of SOCS3. PMID: 24833710
  99. High levels of STAT3 expression were associated with early stages of breast cancer development and patients in the control group with obesity showed higher expression of SOCS3 regarding overweight PMID: 24932998
  100. miR-221 directly inhibits the expression of SOCS3 and IRF2. PMID: 24607843

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Involvement in disease There is some evidence that SOCS3 may be a susceptibility gene for atopic dermatitis linked to 17q25. SOCS3 messenger RNA is significantly more highly expressed in skin from patients with atopic dermatitis than in skin from healthy controls. Furthermore, a genetic association between atopic dermatitis and a haplotype in the SOCS3 gene has been found in two independent groups of patients.
Tissue Specificity Widely expressed with high expression in heart, placenta, skeletal muscle, peripheral blood leukocytes, fetal and adult lung, and fetal liver and kidney. Lower levels in thymus.
Database Links

HGNC: 19391

OMIM: 604176

KEGG: hsa:9021

STRING: 9606.ENSP00000330341

UniGene: Hs.527973

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