Human transthyretin(TTR) ELISA kit

Instructions
Code CSB-E11169h
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name transthyretin
Alternative Names HsT2651, PALB, TBPA, prealbumin, amyloidosis type I|thyroxine-binding prealbumin
Abbreviation TTR
Uniprot No. P02766
Species Homo sapiens (Human)
Sample Types serum, plasma, tissue homogenates
Detection Range 1.56 μg/mL-25 μg/mL
Sensitivity 0.78 μg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Competitive
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of human TTR in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:100 Average % 90  
Range % 87-97  
1:200 Average % 97  
Range % 93-104  
1:400 Average % 85  
Range % 82-91  
1:800 Average % 90  
Range % 85-97  
Recovery
The recovery of human TTR spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 89 85-93  
EDTA plasma (n=4) 92 88-94  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
μg/ml OD1 OD2 Average    
25 0.165 0.162 0.164    
12.5 0.311 0.305 0.308    
6.25 0.612 0.606 0.609    
3.12 0.856 0.849 0.853    
1.56 1.583 1.575 1.579    
0 2.715 2.690 2.703    
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Thyroid hormone-binding protein. Probably transports thyroxine from the bloodstream to the brain.
Involvement in disease Amyloidosis, transthyretin-related (AMYL-TTR); Hyperthyroxinemia, dystransthyretinemic (DTTRH); Carpal tunnel syndrome 1 (CTS1)
Subcellular Location Secreted, Cytoplasm
Protein Families Transthyretin family
Tissue Specificity Detected in serum and cerebrospinal fluid (at protein level). Highly expressed in choroid plexus epithelial cells. Detected in retina pigment epithelium and liver.
Database Links

HGNC: 12405

OMIM: 105210

KEGG: hsa:7276

STRING: 9606.ENSP00000237014

UniGene: Hs.427202

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