Mouse β-catenin,β-cat ELISA Kit

Instructions
Code CSB-E11307m
Size 96T,5×96T,10×96T
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Product Details

Target Name catenin (cadherin-associated protein), beta 1, 88kDa
Alternative Names Ctnnb1 ELISA Kit; CatnbCatenin beta-1 ELISA Kit; Beta-catenin ELISA Kit
Abbreviation CTNNB1
Uniprot No. Q02248
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates, cell lysates
Detection Range 0.156 ng/mL-10 ng/mL
Sensitivity 0.039 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse β-cat in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %92
Range %87-96
1:2Average %99
Range %95-103
1:4Average %101
Range %94-106
1:8Average %95
Range %89-98
Recovery
The recovery of mouse β-cat spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9186-98
EDTA plasma (n=4)9992-106
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
102.854 2.961 2.908 2.715
52.156 2.244 2.200 2.007
2.51.433 1.498 1.466 1.273
1.250.774 0.768 0.771 0.578
0.6250.457 0.443 0.450 0.257
0.3120.324 0.339 0.332 0.139
0.1560.245 0.228 0.237 0.044
00.189 0.197 0.193
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

Target Data

Function Key downstream component of the canonical Wnt signaling pathway. In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes. Involved in the regulation of cell adhesion, as component of an E-cadherin
Gene References into Functions
  1. O-GlcNAcylation of CTNNB1 is associated with tumorigenicity of colorectal cancer. PMID: 29391154
  2. this study identified activation of beta-catenin-mediated signaling in cardiac macrophages post-myocardial infarction PMID: 29356094
  3. This study reports that the gain-of-function mutation of full-length basally-expressing Htt in Huntington's disease cell Q111 (STHdhQ111/HdhQ111) upregulated microRNA-214 and decreased beta catenin & its transcriptional activity in an aggregate-independent manner. PMID: 29894684
  4. beta-catenin plays an essential role in differentiation and function of ameloblasts during amelogenesis PMID: 30066216
  5. Janus kinase 2 protein (JAK2) and beta-catenin were found to interact with cadherin-22 (Cdh22) and involved in CDH22 signaling in female germ line stem cells (FGSCs). PMID: 29063123
  6. In fibrocystin/polyductin complex-defective cholangiocytes, beta-catenin and IL-1beta are responsible for signal transducer and activator of transcription 3-dependent secretion of CXCL10 PMID: 29140564
  7. Data illustrates a cooperative effect of the direct oncogenic signaling of mutant beta-catenin and MET in hepatocytes with hepatic tumor-promoting stroma induced by beta-catenin deficiency PMID: 29152756
  8. Data show that beta-catenin can physically interact with Polycomb Repressive Complex 2 (PRC2) components in the cranial mesenchyme (CM). PMID: 29223978
  9. Study identified FXR/beta-catenin interaction whose modulation through beta-catenin suppression promotes FXR activation and decreases hepatic bile acids, which may provide unique therapeutic opportunities in cholestatic liver diseases PMID: 28714273
  10. In this study, we found that the wnt co-receptor Lrp6 was a potent positive regulator of beta-catenin signaling in TDI-induced asthma models, both in vivo and in vitro. Additionally, for the first time, we demonstrated that RAGE could mediate phosphorylation of Lrp6, suggesting a functional cross talk between RAGE and the canonical wnt/beta-catenin signaling pathway involved in mediating beta-catenin activation. PMID: 29656209
  11. These data indicate that SMAD3- and beta-catenin-dependent induction occurs in the taurine transporter knockout mouse PMID: 28849477
  12. beta-catenin in osterix-expressing cells is required for postnatal osteoblast differentiation, osteoblast proliferation, and bone resorption, and is essential for the anabolic actions of parathyroid hormone in bone. PMID: 29124436
  13. Adherens as well as tight junction marker proteins were rapidly and consistently upregulated in both the germinal as well as the functional layer of the oral mucosa. This represents a previously unknown parameter of the epithelial radiation response to clinically relevant fractionation protocols. CONCLUSION: Fractionated irradiation significantly enhanced the expression of all proteins investigated. This study revealed a PMID: 29675597
  14. the beta-catenin C-terminus indirectly represses p53, and this function is essential for embryogenesis. PMID: 27499244
  15. aberrant expression of AF1q may activate Wnt/beta-catenin signaling and result in podocyte injury. PMID: 29069662
  16. this study suggested that stabilized beta-catenin ameliorated some Autoimmune lymphoproliferative syndrome-like symptoms of lpr/lpr mice by potentiating Fas-independent signal-mediated T cell apoptosis PMID: 29250557
  17. findings indicate that beta-catenin signaling plays a critical role in postnatal bone remodeling. Our study provides new insights into the regulation of epiphyseal bone homeostasis at postnatal stage PMID: 29230102
  18. Study validates the DNAJB1-PRKACA fusion kinase as an oncogenic driver and candidate drug target for fibrolamellar hepatocellular carcinoma in mouse model in which tumorigenesis was significantly enhanced by genetic activation of beta-catenin. PMID: 29162699
  19. BCAS2 is an upstream regulator of beta-catenin gene expression and plays a role in dendrite growth at least partly through beta-catenin in the forebrain. PMID: 27713508
  20. LncRNA MALAT1 is dysregulated in diabetic nephropathy and involved in high glucose-induced podocyte injury via its interplay with beta-catenin and SRSF1. PMID: 28444861
  21. These findings suggest a suppressive function for Ctbp2 in reducing the protein level of beta-catenin, along with priming its position on core pluripotency genes to hinder beta-catenin deposition, which is central to commitment to the appropriate lineage. PMID: 29026198
  22. Beta-catenin deletion in cathepsin K (CtsK)-expressing cells causes a severe loss of bone mass. PMID: 27804995
  23. the GSK-3beta/beta-catenin signal pathway mediates the adverse effects of Ti particles on osteoblast differentiation and bone destruction PMID: 28617442
  24. HIV and drug abuse mediate astrocyte senescence in a beta-catenin-dependent manner leading to neuronal toxicity. PMID: 28612507
  25. The absence of hepatic beta-catenin predisposes mice to hepatic injury and fibrosis following iron overload, which was reminiscent of hemochromatosis and associated with enhanced steatohepatitis and fibrosis. PMID: 28341391
  26. beta-catenin and p65 are activated in separate cellular compartments during liver regeneration, with p65 activity in nonparenchymal compartment contributing to the activation of hepatocyte beta-catenin, cyclin D1 expression, and subsequent proliferation PMID: 28474571
  27. beta-catenin mutations were not involved in early-stage hepatocarcinogenesis induced by protoporphyrinogen oxidase inhibitors PMID: 28580885
  28. the inhibition of beta-catenin's TCF-dependent transcriptional activity, independent of its protein expression level, retains the naive ground state pluripotency in mouse embryonic stem cells. PMID: 28577307
  29. Beta-catenin signaling plays an important part in protecting renal fibrogenesis. PMID: 27460630
  30. Rspo3-LGR4 axis protects hepatocytes from hypoxia/reoxygenation injury via activating beta-catenin. PMID: 29555474
  31. Modulation of beta-catenin levels or disruption of its cell adhesion role is correlated with aggressive tumorigenesis in basal ErbB2-driven mammary tumors with preexisting beta-catenin activation. Our data also emphasize the tumor suppressor role of beta-catenin, similar to other adherens junction proteins, in maintaining junctional integrity during tumor progression. PMID: 28096336
  32. study uncovers a role for Pdpn in mammary SC function and, importantly, identifies Pdpn as a new regulator of Wnt/beta-catenin signaling, a key pathway in mammary development and tumorigenesis PMID: 29361573
  33. CEACAM1 controls epithelial-mesenchymal transition in vitro and in vivo by site-specific regulation of beta-catenin phosphorylation PMID: 27572314
  34. beta-catenin (CTNNB1) maintains lung progenitors by promoting a hierarchical lung progenitor gene signature, suppressing gastrointestinal (GI) genes, and regulating NK2 homeobox 1 (NKX2.1) and SRY box 2 (SOX2) in a developmental stage-dependent manner. PMID: 29440304
  35. findings provide the first line of evidence that links beta-catenin function to the cell proliferation and progenitor establishment during larynx and vocal fold development. PMID: 29386246
  36. In mice that were separated from their mothers, cdh-1 is upregulated in the prefrontal cortex in both males and females. In addition, hippocampal CDH-1 mRNA levels were positively correlated with recognition memory performance in females. Maternal-separated reared male mice exhibited higher beta -Cat mRNA levels in the hippocampus. PMID: 28435022
  37. PTEN-beta-catenin signaling is a novel regulator involved in modulating Treg development and may lead to a potential therapeutic target in liver ischemia/reperfusion injury PMID: 28152578
  38. These results indicate that Wnt/beta-catenin signaling may play a key role in fibrotic scar formation after traumatic spinal cord injury. PMID: 29410176
  39. Data show that phospholipase C (PLC)-beta1 (PLC-beta1) overexpression determines an increase in beta-catenin translocation and that PLC-beta1, inositol polyphosphate multikinase (IPMK) and beta-catenin are mediators of the same signaling pathway. PMID: 27563828
  40. FGF23 promotes myocardial fibrosis and exacerbates diastolic dysfunction induced by myocardial infarction or ischemia/reperfusion , which is associated with the upregulation of active beta-catenin and TGF-beta PMID: 27579618
  41. Suggest that Pygo2 has a tumor promoting function related to Wnt/ss-catenin signaling activity during intestinal tumor initiation and progression. PMID: 27811361
  42. Data suggest that Wnt signaling directs fatty acid metabolism via canonical mechanism involving activation of beta-catenin in osteoblasts; beta-catenin accumulation and activation are required for normal lipid catabolism in osteoblasts and white adipose tissue. PMID: 29077850
  43. Stretch induced p120 degradation and the endocytosis of E-cadherin, which induced beta-catenin translocation into the nucleus, a key event in lung injury progress and repair. PMID: 27911872
  44. Expression of a dominant stable form of beta-catenin in hepatocytes results in severe cholestasis and biliary type fibrosis. PMID: 27895309
  45. These data suggested that Wnt/beta-catenin pathway might be a potential target to treat the LPS-induced inflammation in ALI. PMID: 29246763
  46. Data show that Wnt beta-Catenin signaling pathway activation followed by Notch inhibition strongly promotes the mitotic regeneration of new hair cell (HC) in both normal and neomycin-damaged cochleae. PMID: 27564256
  47. KY1022, a small molecule that destabilizes both beta-catenin and Ras by targeting the Wnt/beta-catenin pathway, inhibitits cellular events, including epithelial mesenchymal transformation, an initial process of metastasis, and apoptosis in colorectal cancer cells. PMID: 27835580
  48. Cardiomyocyte hypertrophy is blunted with cardiac fibroblast-specific loss of beta-catenin after trans-aortic constriction in vivo. PMID: 28959037
  49. These results collectively suggest that sustained activation of Wnt/beta-catenin signaling in endothelial cells might be a cause of heart failure through suppressing neuregulin-ErbB signaling. PMID: 27146149
  50. Our findings indicate that H2O2 inhibits NaV1.5 expression by activating the Wnt/b-catenin signaling and beta-catenin interacts with TCF4 to transcriptionally suppress cardiac NaV1.5 expression. PMID: 27068063
  51. TGF-beta and beta-catenin crosstalk in proximal tubules may have a role in tubular injury in two models of chronic kidney disease PMID: 28701516
  52. Results indicate that ablation of epithelial Wnt/beta-catenin signaling affected epididymal epithelial cell proliferation but did not inhibit cell differentiation. PMID: 29029059
  53. Interactions between the Wnt/beta-catenin and the Kras/ERK/Foxm1 pathways are essential to restrict SOX9 expression in basal cells during pulmonary branching morphogenesis PMID: 26869074
  54. The collective results indicate that the osteoanabolic response to loading can occur on the periosteal surface when beta-cat levels are significantly reduced in Dmp1-expressing cells. PMID: 27143110
  55. L. donovani triggered AKT activation to regulate GSK-3beta/beta-catenin/FOXO-1 axis. PMID: 27662364
  56. NFkB/beta-catenin signaling pathway plays a key role in the regulation of breast cancer-induced bone cell activity and osteolysis. PMID: 28965856
  57. Knockdown of MACF1 in osteoblastic cells inhibits osteoblast differentiation through suppressing the beta-catenin/TCF1-Runx2 axis. PMID: 28621459
  58. Transcriptional cofactors Bcl9, Bcl9l and Pygo1/2 act independently of beta-catenin to ensure proper enamel formation. PMID: 28174279
  59. Transient expression of WNT2 promotes somatic cell reprogramming by inducing beta-catenin nuclear accumulation. PMID: 27211212
  60. Beta-catenin regulates expression of downstream targets of a key transcriptional memory gene, Hoxa9 that is highly enriched in myeloid leukemia cancer stem cells and helps sustain leukemic self-renewal. PMID: 28978671
  61. Under diabetic oxidative stress or H2O2 stimulation, nuclear beta-catenin accumulation upregulated downstream c-Myc and further facilitated DNA damage and p53-mediated apoptosis as well as cell viability reduction, followed by phenotypic changes of cardiac dysfunction, interstitial fibrosis deposition and myocardial atrophy. PMID: 28989026
  62. direct overexpression of Foxl2 decreased the expression of Sertoli cell-specific genes in primary Sertoli cells. Taken together, these results demonstrate that repression of beta-catenin (CTNNB1) signaling is required for lineage maintenance of Sertoli cells. PMID: 28900034
  63. Results demonstrate a new mechanism for the modulation of synapse formation, whereby MET activation induces an alignment of presynaptic and postsynaptic elements that are necessary for assembly and formation of functional synapses by subsets of neocortical neurons that express MET/beta-catenin complex. PMID: 27595133
  64. Following transepithelial migration, neutrophils adhesion to ICAM-1 resulted in activation of Akt and beta-catenin signaling, increased epithelial-cell proliferation, and wound healing. PMID: 26732677
  65. receptor for advanced glycation end products (RAGE) was required for stabilization of beta-catenin in toluene diisocyanate-induced asthma, identifying protective effects of RAGE blockade in this mouse model PMID: 27332707
  66. Axud1 mediated stress-induced cardiomyocytes apoptosis through activating Wnt/beta-catenin signaling pathway. PMID: 28830684
  67. Our findings are consistent with published reports wherein anterior taste buds have higher sweet sensitivity while posterior taste buds are better tuned to bitter, and suggest beta-catenin plays a greater role in renewal of anterior versus posterior taste buds. PMID: 28846687
  68. Exogenous or paracrine sources of NO promote the specification towards the myocyte lineage and expression of cardiac sarcomeric proteins of adult cardiac progenitor cells. This is contingent upon the expression and activity of the alpha1 subunit of guanylyl cyclase in CPC that is necessary for NO-mediated inhibition of the canonical Wnt/beta-catenin pathway. PMID: 27520736
  69. AP-2 beta and beta-catenin interact both in vitro through GST pull-down assays and in vivo by co-immunoprecipitation. We further identified the interaction regions to the DNA-binding domain of AP-2 beta and the 1-9 Armadillo repeats of beta-catenin. PMID: 28277615
  70. TIEG1 is involved in regulating the canonical Wnt signaling pathway in bone through multiple mechanisms of action. PMID: 28201653
  71. Activation of WNT/b-catenin activity improved cardiac contractility and ameliorated intraventricular conduction defects in LmnaH222P/H222P mice, which was associated with increased expression of myocardial connexin 43. These results indicate that decreased WNT/b-catenin contributes to the pathophysiology of LMNA cardiomyopathy and that drugs activating b-catenin may be beneficial in affected individuals PMID: 28069793
  72. Oncogenic beta-catenin and PI3K activities interact in the acquisition of multiple cancer-related phenotypes in organoids grown in Matrigel. PMID: 28442534
  73. We further found that knockdown of Kif2a decreased the protein level of b-catenin, which is a critical molecule for neocortical neurogenesis. Together, these results reveal an important function of Kif2a in embryonic neocortical neurogenesis PMID: 28591194
  74. Data show that both Wnt1-cre and P0-cre are similarly effective in deleting beta-catenin in the neural crest. PMID: 27184910
  75. HIRA, in cooperation with Setd1A, modulates beta-catenin expression to regulate neural stem cell proliferation and neurogenesis. PMID: 28515277
  76. Postnatal beta-catenin ablation in fibroblasts promoted hair follicle (HF) regeneration in neonatal and adult mouse wounds, whereas beta-catenin activation reduced HF regeneration in neonatal wounds. PMID: 27287810
  77. In the absence of the zona pellucida, embryos lacking CTNNB1 undergo fission and these separated blastomeres can become small trophoblastic vesicles, which in turn induce decidual reactions. PMID: 27246714
  78. These results support the notion that pharmacological modulation of beta-catenin can be used to treat pseudarthrosis in patients with neurofibromatosis type 1. PMID: 27306335
  79. we revealed miR-375-3p negatively regulated osteogenesis by targeting LRP5 and beta-catenin PMID: 28158288
  80. our results are consistent with an epithelial proliferative growth mechanism linking CTNNB1-driven Ccnd1 transcription and estrogen-mediated CCND1 protein stabilization. PMID: 27918915
  81. Rbm46 regulates mouse embryonic stem cell differentiation through down-regulation of beta-Catenin.Rbm46 regulates mouse embryonic stem cell differentiation by targeting beta-Catenin mRNA for degradation. PMID: 28212427
  82. Beta-catenin may promote bacterial killing via suppression of P. aeruginosa-induced macrophage autophagy. PMID: 28415949
  83. Data suggest that PKCe positively regulates beta-catenin expression and stabilization in a glycogen synthase kinase 3beta-independent manner; beta-catenin exhibits a perinuclear localization pattern in podocytes; however, beta-catenin is predominantly localized to nucleus in podocytes from PKCe knockout mice. (PKCe = protein kinase C epsilon) PMID: 28539358
  84. Leukocyte beta-catenin expression is disturbed in systemic lupus erythematosus in patients and lupus-prone mice. PMID: 27548498
  85. We have identified cooperation of hMet and beta-catenin activation in a subset of hepatocellular cancer patients and modeled this human disease in mice with a significant transcriptomic intersection; this model will provide novel insight into the biology of this tumor and allow us to evaluate novel therapies as a step toward precision medicine PMID: 27097116
  86. GLP-1 promoted adipogenesis through the modulation of the Wnt4/beta-catenin signaling pathway PMID: 27504979
  87. Study demonstrates a critical role of presynaptic cadherin/catenin/p140Cap cell adhesion complexes in stabilizing functional synapses and spines in the developing neocortex. PMID: 28641114
  88. YAP2-5SA-DeltaC drives beta-catenin activity to promote basal keratinocyte proliferation in the mouse skin in vivo PMID: 27816394
  89. the gliogenic property of Olig1-positive progenitors in forebrain can be efficiently switched to neurogenic by over-expressing beta-catenin, revealing a neurogenic effect of beta-catenin in the forebrain Olig1-positive progenitors. PMID: 27084689
  90. Transcriptome analysis of AKT-CAT tumors revealed that cellular growth and proliferation were mainly affected by chronic inflammation and caused up-regulation of Cxcl16, Galectin-3, and Nedd9, among others. PMID: 26844528
  91. TCF7L2 mediates canonic Wnt/beta-catenin signaling and c-Myc upregulation during abnormal cardiac remodeling in heart failure and suppression of Wnt/beta-catenin to c-Myc axis can be explored for preventing and treating heart failure. PMID: 27301468
  92. lung myeloid cells are responsive to Lrp5/beta-catenin signaling, leading to differentiation of an alveolar macrophage subtype that antagonizes the resolution of lung fibrosis. PMID: 27668462
  93. Vascular smooth muscle beta-catenin is required for formation of neointima after vascular injury. PMID: 28302627
  94. Fzd7 expressed by endothelial cells drives postnatal angiogenesis via activation of Dvl/beta-catenin signaling and can control the integrative interaction of Wnt and Notch signaling during postnatal angiogenesis. PMID: 27758766
  95. The data suggest that vitamin D and calcium signaling are necessary components of the epidermal response to wounding, likely by regulating stem cell activation through increased beta-catenin transcriptional activity. PMID: 26282157
  96. Data show that methyl gallate (MG) prevents beta-catenin degradation during 3T3-L1 adipocytes differentiation. PMID: 27592552
  97. the persistence of p-beta-catenin(Y489) is a durable marker of fibroblast activation in Bronchopulmonary dysplasia and may play an important role in BPD disease pathobiology. PMID: 27941077
  98. Activation of the EP3 receptor facilitates sprouting angiogenesis through protein kinase A/beta-catenin/notch signaling. PMID: 28254818
  99. AT2R inhibits adipogenic differentiation in mesenchymal stem cells. Moreover, this inhibitory effect is associated with Wnt10b/beta-catenin signaling. PMID: 27295344
  100. Beta-catenin signaling controls C4ST-1 gene expression through histone deacetylase. PMID: 27751852

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Subcellular Location Cytoplasm, Nucleus, Cytoplasm, cytoskeleton, Cell junction, adherens junction, Cell junction, Cell membrane, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, Cytoplasm, cytoskeleton, spindle pole, Cell junction, synapse
Protein Families Beta-catenin family
Tissue Specificity Expressed in cerebellar granule neurons (at protein level).
Database Links

KEGG: mmu:12387

STRING: 10090.ENSMUSP00000007130

UniGene: Mm.291928

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