Mouse Angiopoietin 2,ANG-2 ELISA Kit

Code CSB-E07305m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

This Mouse ANGPT2 ELISA Kit was designed for the quantitative measurement of Mouse ANGPT2 protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 0.156 pg/mL-10 pg/mL and the sensitivity is 0.039 pg/mL .

Target Name angiopoietin 2
Alternative Names Angpt2 ELISA Kit; Agpt2Angiopoietin-2 ELISA Kit; ANG-2 ELISA Kit
Abbreviation ANGPT2
Uniprot No. O35608
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 0.156 pg/mL-10 pg/mL
Sensitivity 0.039 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ANG-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:20Average %92
Range %88-95
1:40Average %105
Range %100-107
1:80Average %93
Range %87-97
1:160Average %97
Range %91-101
Recovery
The recovery of mouse ANG-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9589-98
EDTA plasma (n=4)9790-100
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
102.427 2.411 2.419 2.250
51.988 2.022 2.005 1.836
2.51.354 1.379 1.367 1.198
1.250.780 0.742 0.761 0.592
0.6250.495 0.464 0.480 0.311
0.3120.362 0.377 0.370 0.201
0.1560.265 0.255 0.260 0.091
00.171 0.167 0.169  
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

Citations

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Target Background

Function
(From Uniprot)
Binds to TEK/TIE2, competing for the ANGPT1 binding site, and modulating ANGPT1 signaling. Can induce tyrosine phosphorylation of TEK/TIE2 in the absence of ANGPT1. In the absence of angiogenic inducers, such as VEGF, ANGPT2-mediated loosening of cell-matrix contacts may induce endothelial cell apoptosis with consequent vascular regression. In concert with VEGF, it may facilitate endothelial cell migration and proliferation, thus serving as a permissive angiogenic signal.
Gene References into Functions
  1. Flunarizine protected ECs from TNFalpha-induced increase in Angpt-2 transcription and vascular barrier breakdown. PMID: 28276491
  2. At the molecular level NLGN2 colocalizes with its neuronal partner collibystin, a CDC42 guanine nucleotide exchange factor, which is also expressed by endothelial cells and in turn modulates Angiopoietin 2 release. PMID: 29709479
  3. ANGPT2-TIE2 signaling is essential for mesenteric lymphatic development.VEPTP (vascular endothelial protein tyrosine phosphatase) determines TIE2 response to ANGPT2. PMID: 29358379
  4. Endothelial glycocalyx breakdown is mediated by Angpt-2 in a non-redundant manner. PMID: 28453727
  5. These results indicate that the Angpt-Tie2 system is essential for SC integrity. The impairment of this system underlies POAG-associated pathogenesis, supporting the possibility that Tie2 agonists could be a therapeutic option for glaucoma. PMID: 28920924
  6. ANG2 activation of Tie2 supports stable enlargement of normal nonleaky vessels, but reduction of Tie1 in inflammation leads to ANG2 antagonism of Tie2 and initiates a positive feedback loop wherein FOXO1-driven ANG2 expression promotes vascular remodeling and leakage PMID: 27548529
  7. A balanced expression of the Ang1/Ang2 system is important for islet physiology. Ang-2 prevents beta-cell mass and islet vascular adaptation in response to HFD feeding with no major influence on glucose homeostasis PMID: 27617438
  8. These results underscore a pivotal role of Kaposi's sarcoma-associated herpesvirus -induced Ang-2 in KS tumor development by promoting both angiogenesis and inflammation. PMID: 27294705
  9. NDPKB is a protective factor in the retina, which controls Ang2 expression and the hexosamine pathway. PMID: 25900369
  10. miR-150 is a novel suppressor of Ang2 generation with a key role in resolving vascular injury and reducing mortality resulting from sepsis. PMID: 26743170
  11. Ang-2 blockage was beneficial as it decreased fatty streak formation and plasma triglyceride levels, but had no adverse effect on pre-existing atherosclerosis in hypercholesterolemic mice. PMID: 26062989
  12. The results establish Ang2-mediated beta1-integrin activation as a promoter of endothelial destablization, explaining the controversial vascular functions of Ang1 and Ang2. PMID: 25635707
  13. Alprostadil treatment can protect renal function by reducing proteinuria. These effects are mediated, at least in part, through down-regulation of Ang-2 and IL-18 expression PMID: 25200363
  14. ANG2 as the first essential regulator of the functionally important interendothelial cell-cell junctions that form during lymphatic development PMID: 25030698
  15. This study identifies angiopoietin-2 as a link between kidney fibrosis and arterial stiffness. PMID: 24511140
  16. vitamin D-VDR signaling prevents lung injury by blocking the Ang-2-Tie-2-MLC kinase cascade and the renin-angiotensin system. PMID: 24196349
  17. Angiopoietin-2 was expressed on lymphatic vessels and macrophages in inflamed cornea. While corneal LG response was abolished in Ang-2 knockout mice. PMID: 24781940
  18. The Ang-1, Ang-2, Tie-2 system takes part in the pathologic processes in the brains that suffered acute carbon monoxide poisoning by participating in neovascularization. PMID: 23424209
  19. Anti-angiogenic miR-542-3p directly targets the key angiogenesis-promoting protein Angpt2 to prevent tumor angiogensis. PMID: 24403060
  20. Data report here in a definite genetic setting that Ang-2 is critical for multiple vascular permeability-inducing cytokines. PMID: 23940579
  21. Ang-2 is involved in initial steps of the triple-negative breast cancer brain metastasis cascade PMID: 24421076
  22. The data establish liver sinusoidal endothelial cells (LSECs) as a dynamic rheostat of liver regeneration, spatiotemporally orchestrating hepatocyte and LSEC proliferation through angiocrine- and autocrine-acting Ang2, respectively. PMID: 24458641
  23. Myocardial upregulation of angiopoietin 2 is attenuated following thrombospondin-1 disruption. PMID: 24081879
  24. angiopoietin-2 deficiency do not protect mice against endotoxin-induced acute kidney injury. PMID: 22872727
  25. a protective role of Ang2 following cerebral ischemia, an action associated with a reduced BBB permeability. PMID: 23211963
  26. LRP5-Tie2-Ang signaling axis plays a central role in control of both angiogenesis and alveolarization during postnatal lung development PMID: 22848540
  27. Ang-2 may be involved in follicular development, atresia, ovulation, and corpus luteum formation and regression. PMID: 23350236
  28. During liver colonization showed, colony growth and neo-angiogenesis were strongly affected by host Ang2, specifically, larger tumor volume, higher vascular density and pericyte coverage development in its absence. PMID: 22699974
  29. a contextual model whereby differential TIE2 and integrin expression, binding, and activation control the role of ANG-2 in angiogenesis. PMID: 22585576
  30. Studies indicate an important role of Ang2 in pancreatic vascular defects induced by hyperglycaemia. PMID: 22272235
  31. In addition to its role during setup of the brain vasculature, Ang2 is also involved in embryonic cortical neurogenesis and in the radial migration of projection neurons. PMID: 21127017
  32. Results indicate that blocking Ang2 inhibits metastatic dissemination in part by enhancing the integrity of endothelial cell-cell junctions. PMID: 22343031
  33. Ang-2 acts as a crucial regulator of inflammatory lymphangiogenesis by sensitizing the lymphatic vasculature to inflammatory stimuli, thereby directly promoting lymphangiogenesis. PMID: 22058148
  34. Studies suggest that the combined targeting of angiogenic ECs and proangiogenic TEMs by selective ANG2/TIE2-pathway inhibitors may extend the reach of vascular-targeting therapies in cancer patients. PMID: 21576085
  35. human Ang2 antibodies are potentially therapeutic agents for ocular neovascularization in models of retinal and choroidal neovascularization, in the absence of VEGF inhibition. PMID: 21851472
  36. Ang-2 is a novel, endothelial-derived regulator of myeloid cell infiltration that modulates beta-integrin-mediated adhesion in a paracrine manner. PMID: 21868579
  37. In cultured brain endothelial cells exposed to hypoxia,expression of Ang-2, MMP2 and caspase 3 were elevated. PMID: 21904637
  38. results showed Ang-2-transfected tumours displayed aberrant angiogenic vessels with few associated smooth muscle cells. No detectable differences in VEGF expression were observed between Ang-2-transfected and parental tumours. PMID: 21163623
  39. Ang-1 treatment downregulates pulmonary inflammation, VEGF and Ang-2 expression but does not protect against vascular leakage and impaired gas exchange induced by HVT-ventilation PMID: 21179479
  40. Ang-2 exacerbated mouse heart myocardial endothelial cells dysfunction induced by high glucose condition. PMID: 21113176
  41. increased expression of Tie2 and Ang2 in allergic airway epithelium and alveolar macrophages, but not Ang1 or VEGF correlates with the severity of airway remodeling PMID: 20463289
  42. Ang2 acting as an antagonist of Tie2 receptors and the reduction of Tie2 phosphorylation in endothelial cells rendering the vasculature more responsive to cytokines that promote both vascular remodeling and inflammation after M. pulmonis infection. PMID: 20952594
  43. Angiopoietin 2 mediates inflammatory hemangiogenesis, lymphangiogenesis and neutrophil infiltration in dextran sodium sulfate-induced colitis. PMID: 19902545
  44. Findings provide evidence that the inflammatory cytokine oncostatin M is involved in modulation of the Ang-Tie system by increasing Ang2 expression in human endothelial cells in vitro, and in murine hearts and human hearts in vivo. PMID: 20088942
  45. Data suggest that inhibition of Angiopoietin 2 leads to normalization of tumor blood vessels by permitting the unopposed action of Ang1, but decreases tumor vascularity primarily by blocking Ang2 actions. PMID: 19815705
  46. data show that tumor cell aggregates initiate vascular growth by angiogenic sprouting via the simultaneous expression of VEGFR-2 and Ang-2 by host and tumor endothelium PMID: 11901186
  47. Orchestrates angiogenesis and arteriovenous contribution PMID: 12048246
  48. Angiopoietin-2 plays an important role in retinal angiogenesis PMID: 12115724
  49. Ang2 signals are required for the proper development and function of the lymphatic vessels. PMID: 12361591
  50. Angiopoetin 2 is required for postnatal angiogenesis and lymphatic patterning. PMID: 12361603

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Subcellular Location Secreted.
Tissue Specificity Expressed only at sites of vascular remodeling.
Database Links

KEGG: mmu:11601

STRING: 10090.ENSMUSP00000033846

UniGene: Mm.439874

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