Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CD63 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
The recovery of mouse CD63 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Average % Recovery
EDTA plasma (n=4)
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
What is the typical sample dilution for detection in serum?
Thanks for your inquiry! CSB-EL004476HU We tested the mentioned sample stated in the manual. We suggest you follow the manual.If you want to test human fecal samplee ,we suggest you do a pretest . Pls find following sample preparation method. Feces Dilute samples with appropriate Sample Diluent. This can be achieved by adding 0.1g sample to appropriate Sample Diluent. Remove particulates by centrifugation for 10 minutes at 4000x g at 2-8°C and assay immediately. Pls let me know if you have any further questions. Thank you.
Functions as cell surface receptor for TIMP1 and plays a role in the activation of cellular signaling cascades. Plays a role in the activation of ITGB1 and integrin signaling, leading to the activation of AKT, FAK/PTK2 and MAP kinases. Promotes cell survival, reorganization of the actin cytoskeleton, cell adhesion, spreading and migration, via its role in the activation of AKT and FAK/PTK2. Plays a role in VEGFA signaling via its role in regulating the internalization of KDR/VEGFR2. Plays a role in intracellular vesicular transport processes, and is required for normal trafficking of the PMEL luminal domain that is essential for the development and maturation of melanocytes. Plays a role in the adhesion of leukocytes onto endothelial cells via its role in the regulation of SELP trafficking. May play a role in mast cell degranulation in response to Ms4a2/FceRI stimulation, but not in mast cell degranulation in response to other stimuli.
Gene References into Functions
In renal proximal tubules, CD63 determines the insertion of OCT2 into the proper membrane domain and mediates transporter regulation by trafficking processes. PMID: 28031320
TIMP1 signaling via CD63 leads to activation of hepatic stellate cells, which create an environment in the liver that increases its susceptibility to pancreatic tumor cells. PMID: 27506299
The Muted protein is important for the steady-state level of CD63. PMID: 27531610
Data show that CD63 is a crucial player in the regulation of the tumor cell-intrinsic metastatic potential by affecting cell plasticity. PMID: 25354204
These findings identify CD63 as an important component of allergic inflammation. PMID: 23945142
CD63 serves to bridge between beta1 integrin and VEGFR2 because CD63 silencing disrupted VEGFR2-beta1 integrin complex formation identified using proximity ligation assay PMID: 23632027
Loss of CD63 is associated impairment of amyloidogenesis and downstream melanosome morphogenesis. PMID: 21962903
Loss of CD63 has a similar phenotype to loss of P-selectin itself, thus CD63 is an essential cofactor to P-selectin. PMID: 21803846
CXCR4 can be downregulated on activated B cells by IL-21-induced endocytosis and CD63-mediated endosomal recruitment. PMID: 21270405
The findings identify palmitoylation-dependent association with the tetraspanin CD63 as the mechanism by which Syt VII is targeted to lysosomes. PMID: 21041449
CD63, but not RILP, is recruited to the phagosomes in macrophages expressing the dominant negative form of Rab7. PMID: 20236428
CD63 is recruited to already-budded Weibel-Palade bodies by an AP-3-dependent route PMID: 16683915
Upon phagocytosis of Cryptococcus neoformans and polystyrene beads, CD63 was recruited selectively to C. neoformans-containing phagosomes in a MyD88-independent acidification-dependent manner PMID: 17043215
protein region defined by amino acid residues 103-205 for CD63 interacts with amelogenin PMID: 17708745
CD63 knockout mice show an increased urinary flow, water intake, reduced urine osmolality, and a higher fecal water content. PMID: 19075008
the activation-induced degranulation of Fas ligand has distinct requirements and involves different mechanisms than those of the granule markers CD63 and CD107a/Lamp-1 PMID: 19079288