Mouse Lymphotoxin-beta(LTB) ELISA kit

Code CSB-EL013220MO
Size 96T,5×96T,10×96T
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Product Details

Target Name
lymphotoxin beta (TNF superfamily, member 3)
Alternative Names
Ltb; Tnfc; Tnfsf3; Lymphotoxin-beta; LT-beta; Tumor necrosis factor C; TNF-C; Tumor necrosis factor ligand superfamily member 3
Abbreviation
LTB
Uniprot No.
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, tissue homogenates
Detection Range
12.5 pg/mL-800 pg/mL
Sensitivity
3.12 pg/mL
Assay Time
1-5h
Sample Volume
50-100ul
Detection Wavelength
450 nm
Research Area
Immunology
Assay Principle
quantitative
Measurement
Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%      
Three samples of known concentration were tested twenty times on one plate to assess.  
Inter-assay Precision (Precision between assays): CV%<10%      
Three samples of known concentration were tested in twenty assays to assess.    
             
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse LTB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)  
1:1 Average % 90  
Range % 86-94  
1:2 Average % 84  
Range % 80-88  
1:4 Average % 89  
Range % 84-93  
1:8 Average % 98  
Range % 94-102  
Recovery
The recovery of mouse LTB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range  
Serum (n=5) 95 90-99  
EDTA plasma (n=4) 104 100-108  
             
             
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/ml OD1 OD2 Average Corrected  
800 2.704 2.527 2.616 2.466  
400 2.047 2.206 2.127 1.977  
200 1.415 1.504 1.460 1.310  
100 0.704 0.735 0.720 0.570  
50 0.394 0.419 0.407 0.257  
25 0.291 0.279 0.285 0.135  
12.5 0.213 0.205 0.209 0.059  
0 0.154 0.145 0.150    
Troubleshooting
and FAQs
Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description

This Mouse LTB ELISA Kit was designed for the quantitative measurement of Mouse LTB protein in serum, plasma, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 12.5 pg/mL-800 pg/mL and the sensitivity is 3.12 pg/mL.

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Target Background

Function
(From Uniprot)
Cytokine that binds to LTBR/TNFRSF3. May play a specific role in immune response regulation. Provides the membrane anchor for the attachment of the heterotrimeric complex to the cell surface.
Gene References into Functions
  1. The expression of lymphotoxin (LT)-B was significantly downregulated in the absence of T cells from nude mice and was recovered after the transfusion of T cells. PMID: 25266629
  2. TNF is able to upregulate LT-beta expression in hepatic cells at the transcriptional level by the binding of NF-kappaB p50/p65 heterodimers and Ets1 to their respective sites in the LT-beta promoter. PMID: 22742857
  3. Lymphotoxin-beta-receptor (LTbetaR) signaling, a pathway essential for lymphoid organogenesis, abrogates tertiary lymphoid organ development in heart transplantation. PMID: 21926237
  4. LTbeta RNA is detectable in embryos ranging from 5.5 to 18.5 days of development, e.g., in peripheral lymph nodes, Peyer's patches, thymus and skin of the E18.5 embryo and fetal liver of E12.5. PMID: 11994460
  5. membrane lymphotoxin beta contributes to anti-leishmanial immunity by controlling structural integrity of lymphoid organs PMID: 12115620
  6. The organogenic function of B-LTbeta is almost entirely restricted to spleen, where it supports the correct lymphoid architecture that is critical for an effective humoral immune response. PMID: 12354378
  7. microenvironment in peripheral lymphoid organs associated with lymphotoxin alpha/lymphotoxin beta-lymphotoxin beta receptor signaling and chemokine production is critical for recruitment efficiency of dendritic cel PMID: 12560241
  8. membrane LT-beta is important in resistance to Theiler's virus infection PMID: 12882833
  9. Lymphotoxin-mediated adhesion molecule expression may be important in the development of graft-versus-host skin disease. PMID: 14734744
  10. LTbeta, LTbeta receptor, and IFNgamma are involved in oval cell-mediated, but not hepatocyte-mediated, liver regeneration, and the absence of these pathways impairs the oval cell-dependent regenerative response. PMID: 15660390
  11. The lymphotoxin beta signaling pathway is an essential effector pathway for host defense against the beta-herpesvirus muromegalovirus (MCMV). PMID: 15905567
  12. Data show that, as an ectodysplasin target, lymphotoxin-beta regulates hair phenotype in developing hair follicles. PMID: 16738056
  13. Expression of LTbeta on lymphocytes enhances the induction of immune responses against limiting amounts of antigen PMID: 16841297
  14. Expression of lymphotoxin-alphabeta on antigen-specific T cells is required for DC function. PMID: 17452522
  15. Activation of LTbetaR by LTalphabeta mainly expressed on T lymphocytes is crucial for the down regulation of the inflammatory response in this experimental model. PMID: 17590442
  16. LTbeta shows a primary early function in periderm differentiation, with later transient effects on epidermal and hair follicle differentiation. PMID: 17673451
  17. signal was essential for primary B cell cluster formation with initial differentiation of follicular dendritic cells (FDCs) in neonatal mice. PMID: 17982070
  18. LTbetaR on hepatic stellate cells may be involved in signaling with LTbeta-expressing liver progenitor cells mediating recruitment of progenitor cells, hepatic stellate cells, & leukocytes for wound healing & regeneration during chronic liver injury. PMID: 19111021

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Subcellular Location
Membrane; Single-pass type II membrane protein.
Protein Families
Tumor necrosis factor family
Database Links
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