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24T ELISA Kit Trial Size (Only USD$150/ kit)
|Intra-assay Precision (Precision within an assay): CV%<8%|
|Three samples of known concentration were tested twenty times on one plate to assess.|
|Inter-assay Precision (Precision between assays): CV%<10%|
|Three samples of known concentration were tested in twenty assays to assess.|
|To assess the linearity of the assay, samples were spiked with high concentrations of mouse OSTN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.|
|The recovery of mouse OSTN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.|
|Sample Type||Average % Recovery||Range|
|EDTA plasma (n=4)||97||89-105|
|These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.|
This Mouse OSTN ELISA Kit was designed for the quantitative measurement of Mouse OSTN protein in serum, plasma, cell culture supernates, tissue homogenates. It is a Sandwich ELISA kit, its detection range is 15.6 pg/mL-1000 pg/mL and the sensitivity is 3.9 pg/mL.
Sample type: Serum
Sample species: Mouse
Sample dilution: 1:10
Review: This kit is easy to use. A 10-fold dilution of the mouse serum samples gave a range of OD between 0.3 to 0.6. A negative control (mouse BNP) was included and the OD was negative. Thus, this shows that the kit is specific.
Is an extraction step required from Plasma?