Mouse Platelet Factor 4,PF-4 ELISA Kit

Code CSB-E07884m
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Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. Q9Z126
Lead Time 3-5 working days
Protein Biological Process 2 chemokine
Abbreviation PF4
Protein Biological Process 1 Cardiovascular
Target Name platelet factor 4
Alias CXCL4, MGC138298, SCYB4, chemokine (C-X-C motif) ligand 4|platelet factor 4 (chemokine (C-X-C motif) ligand 4)
Species Mus musculus (Mouse)
Protein Biological Process 3 Chemotaxis
Sample Types serum, plasma, tissue homogenates
Detection Range 3.125 ng/mL-200 ng/mL
Sensitivity 1.603 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PF-4 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
  Sample Serum(n=4)
1:200 Average % 86
Range % 81-90
1:400 Average % 98
Range % 92-104
1:800 Average % 97
Range % 94-102
1:1600 Average % 94
Range % 89-98
Recovery
The recovery of mouse PF-4 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample Type Average % Recovery Range
Serum (n=5) 89 86-94
EDTA plasma (n=4) 98 92-104
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/ml OD1 OD2 Average Corrected
200 2.501 2.402 2.452 2.308
100 2.093 1.975 2.034 1.890
50 1.354 1.383 1.369 1.225
25 0.778 0.769 0.774 0.630
12.5 0.386 0.371 0.379 0.235
6.25 0.268 0.257 0.263 0.119
3.125 0.209 0.202 0.206 0.062
0 0.144 0.143 0.144  
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Function Released during platelet aggregation. Neutralizes the anticoagulant effect of heparin because it binds more strongly to heparin than to the chondroitin-4-sulfate chains of the carrier molecule. Chemotactic for neutrophils and monocytes. Inhibits endothelial cell proliferation (By similarity).
Subcellular Location Secreted
Protein Families Intercrine alpha (chemokine CxC) family
Database Links

KEGG: mmu:56744

STRING: 10090.ENSMUSP00000031320

UniGene: Mm.332490

Pathway Chemokine signaling pathway
Lipids and Inflammation in Atherogenesis

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