Mouse Tumor necrosis factor ligand superfamily member 13(TNFSF13) ELISA kit

Instructions
Code CSB-EL023989MO
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application
Have Questions? Leave a Message or Start an on-line Chat

Product Details

Target Name tumor necrosis factor (ligand) superfamily, member 13
Alternative Names Tnfsf13 ELISA Kit; April ELISA Kit; Tumor necrosis factor ligand superfamily member 13 ELISA Kit; A proliferation-inducing ligand ELISA Kit; APRIL ELISA Kit; CD antigen CD256 ELISA Kit
Abbreviation TNFSF13
Uniprot No. Q9D777
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates, cell lysates
Detection Range 93.75 pg/mL-6000 pg/mL
Sensitivity 23.43 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse TNFSF13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %94
Range %86-99
1:2Average %98
Range %90-102
1:4Average %92
Range %85-96
1:8Average %98
Range %92-103
Recovery
The recovery of mouse TNFSF13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9084-94
EDTA plasma (n=4)10091-105
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
60002.636 2.496 2.566 2.455
30002.123 2.089 2.106 1.995
15001.477 1.456 1.467 1.356
7500.776 0.753 0.765 0.654
3750.372 0.366 0.369 0.258
187.50.266 0.254 0.260 0.149
93.750.194 0.187 0.191 0.080
00.112 0.110 0.111
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 5-7 working days

Target Data

Function Cytokine that binds to TNFRSF13B/TACI and to TNFRSF17/BCMA. Plays a role in the regulation of tumor cell growth. May be involved in monocyte/macrophage-mediated immunological processes.
Gene References into Functions
  1. p40, a Lactobacillus rhamnosus-derived protein upregulates EGFR-dependent APRIL expression in intestinal epithelial cells, which may contribute to promoting IgA production PMID: 27353252
  2. this study identifies APRIL as a new target involved in B-cell activation, in the maintenance of plasma cell survival and subsequent increased autoantibody production that sustains lupus development in mice PMID: 28267197
  3. Our data indicate that APRIL contributes to fibrotic scar formation after spinal cord injury by mediating the inflammatory response PMID: 27098833
  4. this is the first study to implicate the APRIL signal transduction pathway in the pathogenesis of nephrogenic IgA production. Moreover, our findings identify APRIL as a potential target of therapy. PMID: 26348210
  5. the elevated presence of APRIL and BLyS in B cell-rich areas of chronically inflamed gingiva suggests that these cytokines may contribute to bone loss by promoting the survival and persistence of RANKL-expressing B cells/plasma cells. PMID: 26150532
  6. myelopoiesis dysregulation characterized by an increased proportion of precursor cells occurs in MM patients. Such dysregulation correlates with a stable expression of the MM-promoting factor APRIL in infiltrated BM. PMID: 25753925
  7. B cell activating factor (BAFF) and a proliferation inducing ligand (APRIL) mediate CD40-independent help by memory CD4 T cells in transplantations. PMID: 25496308
  8. Heteromers consisting of one BAFF and two APRIL bind to receptor TACI, BCMA but not to BAFFR. PMID: 25953898
  9. Immunization of mice with a DNA vaccine encoding BAFF or APRIL multitrimers, together with interleukin 12 and membrane-bound HIV-1 Env gp140, induced neutralizing antibodies against tier 1 and tier 2 (vaccine strain) viruses. PMID: 25631080
  10. APRIL mediates peritoneal B-1 cell homeostasis. PMID: 24512739
  11. APRIL selectively enhances axonal growth by activating ERK signaling and PI3-kinase/Akt/GSK-3beta signaling. PMID: 24444792
  12. Data indicate that APRIL expression accelerates the onset of TCL1-driven leukemia formation mainly through TACI activation. PMID: 24100449
  13. The ability of TACI-Fc to activate reverse signalling through BAFF and APRIL, was investigated. PMID: 23620746
  14. CD1d-restricted invariant NKT cell-derived BAFF and APRIL each contribute to survival of plasma cells induced by immunization. PMID: 23797666
  15. The TNF family member APRIL dampens collagen-induced arthritis. PMID: 23178293
  16. NF-kappaB mediates APRIL-induced HoxC4 transcription. PMID: 23178148
  17. APRIL is dispensable for the development of full-blown SLE in NZM mice. PMID: 22127792
  18. Grafted human colorectal tumor growth and metastasis were inhibited while tumor cell apoptosis and necrosis were induced after in vivo APRIL siRNA injection into nude mice. PMID: 22170570
  19. April production, both in the human and mouse systems, peaks in myeloid precursor cells, before dropping in fully mature granulocytes. PMID: 21642598
  20. Disruption of heparan sulfate proteoglycan conformation perturbs B-cell maturation and APRIL-mediated plasma cell survival. PMID: 21471524
  21. APRIL inhibits allergic lung inflammation by suppressing c-maf expression and directly reducing IL-13 production in CD4+ T cells. PMID: 21182087
  22. Review. Dendritic cell-derived TNF-family ligand APRIL enhances plasmablast survival and differentiation to plasma cells. PMID: 12354377
  23. Results describe the crystal structure of a proliferation-inducing ligand, APRIL. PMID: 15451660
  24. significantly increased APRIL levels in sera of B cell chronic lymphoid leukemia (B-CLL) patients, indicating that APRIL promotes onset of B-1-associated neoplasms PMID: 15488762
  25. heparan sulfate proteoglycans (HSPG) serve as a receptor for APRIL or that HSPG binding allows APRIL to interact with a receptor that promotes tumor growth PMID: 15846369
  26. B cell activation factor from the TNF family and proliferation-inducing ligand can modulate the survival of developing B cells in the bone marrow PMID: 16293214
  27. Review. The splice variants, binding specificities, structural determinants for ligand selectivity, and signaling pathways are reviewed. PMID: 16914324
  28. Review. APRIL interactions with BCMA likely govern memory B cell populations. PMID: 16919470
  29. Review. Direct BAFF/APRIL signalling in T cells and/or T cell modulation in response to a BAFF-modified B cell compartment may play an important role in inflammation and immunomodulation. PMID: 16931039
  30. overexpression of BAFF and APRIL may contribute to the development of autoimmune disease PMID: 17947668
  31. APRIL is a critical signal for plasmablast survival that is poorly expressed in the early-life bone marrow compartment. PMID: 18180376
  32. Long-lived memory B cell survival and function are completely independent of APRIL (a proliferation-inducing ligand) or BAFF (B cell-activating factor of the TNF family). Either APRIL or BAFF supports the survival of bone marrow plasma cells in vivo. PMID: 18322170
  33. These results indicate that APRIL affects specific aspects of the anti-influenza response, but plays a limited role in disease recovery. PMID: 18471884
  34. Erythroleukemia in APRIL transgenic mice is characterized by low hematocrits and grossly enlarged spleens with an increased percentage of erythroid precursors. PMID: 18483203
  35. in APRIL(-/-) mice, the incidence of collagen-induced arthritis was significantly reduced compared with WT mice in parallel with diminished levels of antigen-specific IgG2a autoantibody and IL-17 production PMID: 19016524
  36. APRIL blockade resulted in a significant decrease in IL-4 and eotaxin mRNA and IL-4 and KC protein levels in total homogenates and BAL fluid. PMID: 19037968
  37. IL-4 up-regulates APRIL expression through two divergent pathways in mouse macrophages, p38MAPK-CREB and p38MAPK-Stat6. PMID: 19427796

Show More

Hide All

Subcellular Location Secreted
Protein Families Tumor necrosis factor family
Database Links

KEGG: mmu:69583

STRING: 10090.ENSMUSP00000018896

UniGene: Mm.8983

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

© 2007-2020 CUSABIO TECHNOLOGY LLC All rights reserved. 鄂ICP备15011166号-1