Target Name
cAMP response element binding protein(CREB)
Alternative Names
Creb1 ELISA Kit; Creb-1Cyclic AMP-responsive element-binding protein 1 ELISA Kit; CREB-1 ELISA Kit; cAMP-responsive element-binding protein 1 ELISA Kit
Species
Mus musculus (Mouse)
Sample Types
serum, plasma, cell culture supernates
Detection Range
3.12 pg/mL-200 pg/mL
Detection Wavelength
450 nm
Research Area
Epigenetics and Nuclear Signaling
Assay Principle
quantitative
Precision
Intra-assay Precision (Precision within an assay): CV%<8% | | | |
Three samples of known concentration were tested twenty times on one plate to assess. | |
Inter-assay Precision (Precision between assays): CV%<10% | | | |
Three samples of known concentration were tested in twenty assays to assess. | | |
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Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse CREB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. |
| Sample | Serum(n=4) | |
1:100 | Average % | 95 | |
Range % | 87-103 | |
1:200 | Average % | 94 | |
Range % | 86-105 | |
1:400 | Average % | 96 | |
Range % | 91-101 | |
1:800 | Average % | 90 | |
Range % | 85-100 | |
Recovery
The recovery of mouse CREB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. |
|
Sample Type | Average % Recovery | Range | |
Serum (n=5) | 97 | 83-105 | |
EDTA plasma (n=4) | 95 | 87-101 | |
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Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. |
|
pg/ml | OD1 | OD2 | Average | Corrected | | 200 | 2.786 | 2.723 | 2.755 | 2.619 | | 100 | 2.128 | 2.054 | 2.091 | 1.955 | | 50 | 1.264 | 1.225 | 1.245 | 1.109 | | 25 | 0.772 | 0.809 | 0.791 | 0.655 | | 12.5 | 0.548 | 0.534 | 0.541 | 0.405 | | 6.25 | 0.351 | 0.366 | 0.359 | 0.223 | | 3.12 | 0.239 | 0.224 | 0.232 | 0.096 | | 0 | 0.137 | 0.134 | 0.136 | | |
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Storage
Store at 2-8°C. Please refer to protocol.
Lead Time
3-5 working days after you place the order, and it takes another 3-5 days for delivery via DHL or FedEx
Description
This Mouse CREB ELISA Kit was designed for the quantitative measurement of Mouse CREB protein in serum, plasma, cell culture supernates. It is a Sandwich ELISA kit, its detection range is 3.12 pg/mL-200 pg/mL and the sensitivity is 0.78 pg/mL.