Mouse heme oxygenase 1,HO-1 ELISA Kit

Instructions
Code CSB-E08268m
Size 96T,5×96T,10×96T
See More Details 24T ELISA kits trial application

Product Details

Target Name heme oxygenase (decycling) 1
Alternative Names HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1
Abbreviation HMOX1
Uniprot No. P14901
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %92
Range %89-96
1:200Average %96
Range %91-101
1:400Average %97
Range %93-102
1:800Average %93
Range %87-99
Recovery
The recovery of mouse HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-101
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.416 2.312 2.364 2.173
10001.827 1.906 1.867 1.676
5001.247 1.265 1.256 1.065
2500.799 0.829 0.814 0.623
1250.477 0.486 0.482 0.291
62.50.334 0.362 0.348 0.157
31.250.257 0.269 0.263 0.072
00.194 0.188 0.191  
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days
Still Have Questions? Leave a Message or Start an on-line Chat

Target Data

Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Subcellular Location Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Heme oxygenase family
Database Links

KEGG: mmu:15368

STRING: 10090.ENSMUSP00000005548

UniGene: Mm.276389

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