Mouse heme oxygenase 1,HO-1 ELISA Kit

Code CSB-E08268m
See More Details
Product Type ELISA Kit
Size 96T,5×96T,10×96T
Uniprot No. P14901
Lead Time 3-5 working days
Abbreviation HMOX1
Target Name heme oxygenase (decycling) 1
Alias HO-1, HSP32, bK286B10, heme oxygenase (decyclizing) 1
Species Mus musculus (Mouse)
Sample Types serum, plasma, cell culture supernates, tissue homogenates
Detection Range 31.25 pg/mL-2000 pg/mL
Sensitivity 7.8 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Cardiovascular
Protocol
Protocol may be improved. Please feel free to contact CUSABIO product specialist to obtain the latest version.
Assay Principle quantitative
Measurement Sandwich
Target Details Heme oxygenase, an essential enzyme in heme catabolism, cleaves heme to form biliverdin, which is subsequently converted to bilirubin by biliverdin reductase, and carbon monoxide, a putative neurotransmitter. Heme oxygenase activity is induced by its substrate heme and by various nonheme substances. Heme oxygenase occurs as 2 isozymes, an inducible heme oxygenase-1 and a constitutive heme oxygenase-2. HMOX1 and HMOX2 belong to the heme oxygenase family.
HGNC 5013
RGD 2806
MGI 96163
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse HO-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
 SampleSerum(n=4)
1:100Average %92
Range %89-96
1:200Average %96
Range %91-101
1:400Average %97
Range %93-102
1:800Average %93
Range %87-99
Recovery
The recovery of mouse HO-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9693-101
EDTA plasma (n=4)9185-97
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
pg/mlOD1OD2AverageCorrected
20002.416 2.312 2.364 2.173
10001.827 1.906 1.867 1.676
5001.247 1.265 1.256 1.065
2500.799 0.829 0.814 0.623
1250.477 0.486 0.482 0.291
62.50.334 0.362 0.348 0.157
31.250.257 0.269 0.263 0.072
00.194 0.188 0.191  
References
Still Have Questions? Leave a Message or Start an on-line Chat
Function Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Subcellular Location Microsome, Endoplasmic reticulum membrane, Peripheral membrane protein, Cytoplasmic side
Protein Families Heme oxygenase family
Database Links

KEGG: mmu:15368

STRING: 10090.ENSMUSP00000005548

UniGene: Mm.276389

Pathway HIF-1 signaling pathway

Related Products

Hmox1 Antibodies

Hmox1 Antibodies for Mus musculus (Mouse)

Hmox1 Proteins

Hmox1 Proteins for Mus musculus (Mouse)

Most popular with customers

Newsletters

Get all the latest information on Events, Sales and Offers. Sign up for newsletter today.

Copyright © 2007-2018 www.cusabio.com CUSABIO TECHNOLOGY LLC All Rights Reserved.

Wait!

Join the 25,000 subscribers to get research hotpots, technical tips, latest information on events, sales and offers.

Sign up now!

We don't deal in spam.