Mouse intercellular adhesion molecule 1,ICAM-1 ELISA KIT

Instructions
Code CSB-E04575m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name intercellular adhesion molecule 1
Alternative Names Icam1 ELISA Kit; Icam-1 ELISA Kit; Intercellular adhesion molecule 1 ELISA Kit; ICAM-1 ELISA Kit; MALA-2 ELISA Kit; MyD10 ELISA Kit; CD antigen CD54 ELISA Kit
Abbreviation ICAM1
Uniprot No. P13597
Species Mus musculus (Mouse)
Sample Types serum, plasma, tissue homogenates
Detection Range 78 ng/mL-5000 ng/mL
Sensitivity 19.5 ng/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Immunology
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse ICAM-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
SampleSerum(n=4)
1:1Average %97
Range %91-105
1:2Average %85
Range %80-92
1:4Average %96
Range %85-105
1:8Average %93
Range %83-98
Recovery
The recovery of mouse ICAM-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
Sample TypeAverage % RecoveryRange
Serum (n=5) 9582-101
EDTA plasma (n=4)9486-99
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
ng/mlOD1OD2AverageCorrected
50002.555 2.518 2.537 2.390
25002.047 2.105 2.076 1.929
12501.531 1.552 1.542 1.395
6250.812 0.803 0.808 0.661
3120.516 0.524 0.520 0.373
1560.312 0.321 0.317 0.170
780.229 0.225 0.227 0.080
00.145 0.149 0.147
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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Target Data

Function ICAM proteins are ligands for the leukocyte adhesion protein LFA-1 (integrin alpha-L/beta-2). During leukocyte trans-endothelial migration, ICAM1 engagement promotes the assembly of endothelial apical cups through ARHGEF26/SGEF and RHOG activation (By similarity).
Gene References into Functions
  1. The present study demonstrated that IL-1b may induce ICAM-1 expression, thus enhancing the cohesion between mesenchymal stem cells and endothelial progenitor cells via the p38 MAPK signaling pathway. PMID: 29393395
  2. ICAM-1 overexpressing Mesenchymal Stem Cells could suppress Dendritic Cells maturation according to co-culture methods and suppress the T cell immune response according to the mixed lymphocyte response (MLR) and lymphoblast transformation test (LTT) tests. PMID: 29763906
  3. The results showed that MOVACs transfected with pCMV5-HA-RKIP significantly inhibited TNF-a induced mRNA and protein expression of ICAM-1 and VCAM-1. PMID: 28983072
  4. Endotoxaemia enhanced early venous thrombosis occurs in a TLR-4 and ICAM-1 dependent fashion, and is potentiated by neutropenia. PMID: 27975098
  5. Data show that intercellular adhesion molecule 1 (ICAM-1) and ICAM-2 on B cells are essential for long-lasting cognate T follicular helper (Tfh)-B cell interactions and efficient selection of low-affinity B cell clones for proliferative clonal expansion PMID: 28939548
  6. in T cells, PI3Kdelta attenuates the activation of Rac1, but sustains the activation of Rap1. PMID: 26740009
  7. PTPN22 colocalized with its substrates at the leading edge of cells migrating on surfaces coated with the LFA-1 ligand intercellular adhesion molecule-1 (ICAM-1). PMID: 27703032
  8. Blocking CD54 inhibited NK cell-mediated cytotoxicity of the GM-CSF-stimulated Flt3 ligand conventional dendritic cells. NK cell-mediated lysis of GM-CSF-stimulated cDCs was, in part, a result of increased expression of CD54. PMID: 27034402
  9. These results indicate that, whereas T cells use ICAM-1 and -2 for temporary pulmonary entrapment, neutrophils get sequestered and extravasate into inflamed lungs independent of ICAMs. PMID: 26823454
  10. This study indicates that the ANG II effects are, in part, mediated or triggered by Gal-3 together with the related intercellular signaling (ICAM-1 and IL-6), leading to cardiac inflammation and fibrosis. PMID: 27496875
  11. Data suggest CKIP1 (casein kinase 2 interacting protein-1) plays role in resistance to oxidative stress in glomerular mesangial cells in development of diabetic nephropathy; mechanisms appear to include down-regulation of expression of ICAM1 (intercellular adhesion molecule-1) and fibronectin plus activation of Nrf2/ARE (nuclear factor E2-related factor 2/antioxidant response element) antioxidative stress pathway. PMID: 27481061
  12. ICAM1 regulates pathological cardiac remodeling by mediating proinflammatory leukocyte infiltration in the left ventricle and cardiac fibrosis and dysfunction. PMID: 27068635
  13. Its antiatherogenic impact might be associated with a suppressed adhesion to the endothelium due to down-regulation of endothelial ICAM-1 expression. PMID: 26717272
  14. Luteolin may inhibit tumor angiogenesis and tumor cell proliferation by down-regulation of LFA- 3 and PCNA and up-regulation of ICAM-1 in tumor tissue of tumor-bearing mice, thereby achieving its anti-tumor effect. PMID: 26600503
  15. our results further highlight the pleiotropic role of ICAM-1 in T-cell-dependent immune responses, with a major role in Treg cell development and suppressive function. PMID: 26370005
  16. the present results shed light on regulation of expression and function of ICAM-1 on neutrophils and identify it as an additional regulator of neutrophil effector responses in host defense. PMID: 26647392
  17. we identified intracellular adhesion molecule (ICAM)-1, through fibrinogen binding, as a previously unreported mediator of platelet-monocyte interactions PMID: 26215112
  18. miR-222 was transported into recipient endothelial cells by endothelial microparticles and functionally regulated expression of its target protein ICAM-1 in vitro and in vivo. PMID: 26081516
  19. Timely blockade of ICAM-1.LFA-1 interaction prevents disease onset in a mouse model of emphysema. PMID: 26098520
  20. MRTF-A/B depletion results in an increase in the cell surface expression of ICAM-1 and interactions between HAoECs and leukocytes PMID: 26024305
  21. neutrophils are reliant on endothelial activation for adhesion, initially via E-selectin and subsequently, by integrin-mediated binding to ICAM-1 and VCAM-1, combined with CXCL8-dependent chemotaxis. PMID: 26216937
  22. Inhibition of the P2RX7 pathway not only decreased endothelial ICAM-1 expression and leukocyte adhesion but also prevented microglia overactivation, reduced brain injury, and consequently doubled the early survival of septic mice. PMID: 25998681
  23. ICAM-1 in the tumor microenvironment, via restraining efferocytosis of apoptotic tumor cells, can block M2 macrophage polarization through regulation of PI3K/AKT activation, which leads to prevention of tumor metastasis. PMID: 26068788
  24. Activation of Wnt/beta-catenin signaling in AECs attenuated intercellular adhesion molecule 1 (ICAM-1)/vascular cell adhesion molecule 1 (VCAM-1)-mediated adhesion of both macrophages and neutrophils to AECs. PMID: 26351298
  25. miR-141, as a novel repressor of ICAM-1, is involved in the attenuation of MI/R injury via antithetical regulation of ICAM-1 and inflammatory cell infiltration. PMID: 26371161
  26. ICAM-1 and its localization to myofibers in muscles of mdx mice contributes to the dystrophic pathology. PMID: 25728314
  27. ICAM-1 expression can be visualized with in vivo molecular MRI during EAE, and provides an early tracer of disease activity. PMID: 24753465
  28. Anti-OX40L mAb could prolong secondary heart allograft survival based on CD40/CD40L and LFA-1/ICAM-1 blockade. PMID: 25613092
  29. findings reveal a novel function of ICAM-1 in osteogenesis and suggest a new molecular target to improve bone regeneration and repair in inflammatory microenvironments. PMID: 24702024
  30. ICAM-1 can be considered as a rapid-reacting and sustained gravity-regulated molecule in mammalian cells. PMID: 25654110
  31. This report provides evidence for early-stage regulation of ICAM1 by microRNAs, resulting in reduction of genital pathology associated with chlamydial infection. PMID: 25865776
  32. Caspase-1-independent IL-1 release mediates blister formation in autoantibody-induced tissue injury through modulation of endothelial adhesion molecules. PMID: 25795756
  33. cell surface levels of endothelial ICAM-1 rather than the inflammatory stimulus or blood-brain barrier integrity influence the pathway of T-cell diapedesis across the blood-brain barrier. PMID: 25545837
  34. Constrained ICAM-1 mobility is important for dendritic cell function, as dendritic cells expressing a high-mobility ICAM-1 mutant lacking the cytoplasmic domain exhibit diminished antigen-dependent conjugate formation and T cell priming. PMID: 25666808
  35. Our results indicate that nicotine can enhance the expression of ICAM-1 and VCAM-1 on mouse cardiac vascular endothelial cell via p38 MAPK signaling pathway, resulting in increased expression of the cellular adhesion molecules ICAM-1 and VCAM-1. PMID: 25803997
  36. ICAM-1 expression by skeletal muscle cells augments myogenesis, and establish a novel mechanism through which the inflammatory response facilitates growth processes in skeletal muscle. PMID: 25281303
  37. RAGE and ICAM-1 are required for the anti-inflammatory functions of protein C PMID: 24876676
  38. ICAM-1 has a role in polymicrobial sepsis; its blockade modulates neutrophil migration and reversing immunosuppression PMID: 24891762
  39. Transmigrated neutrophils in the intestinal lumen engage ICAM-1 to regulate the epithelial barrier and neutrophil recruitment. PMID: 24345805
  40. altered expression of ICAM-1 on thymic epithelium shifts autoimmunity specifically toward peripheral nerves. PMID: 25108020
  41. Hydrogen peroxide exposure upregulates ICAM-1 expression in skeletal muscle. PMID: 24333630
  42. Data suggest that PGE2/Ep4 (prostaglandin E receptor 4; Ptger4) signaling via cAMP/Epac (exchange protein directly activated by cAMP 1; Rapgef3) induces expression of Icam1 (intercellular adhesion molecule 1) in cerebrovascular endothelial cells. PMID: 23317035
  43. These data indicate that L-selectin plays a major role in the development of C protein induced polymyositis, whereas ICAM-1 plays a lesser role, if any. PMID: 24644046
  44. TNF-alpha-induced MMP-9 expression may contribute to the production of sICAM-1 by MC3T3-E1 cells. PMID: 24502696
  45. pretreatment with C66 not only significantly reduced high glucose (HG)-induced over-expressions of VCAM-1, ICAM-1 and MCP-1, but also remarkably inhibited NF-kappaB activation, MAPKs phosphorylation PMID: 24260158
  46. ICAM-1 is imperative for inducing regulatory T cells to produce IL-10 in vivo, which would prevent granuloma formation. PMID: 24103557
  47. Data suggest that ICAM-1 isoforms have unique roles in experimental autoimmune encephalomyelitis (EAE). PMID: 24435747
  48. These studies demonstrate that ICAM-1 immunobiology is highly complex but that individual isoforms, aside from the full-length molecule, make significant contributions to disease development and pathogenesis. [review] PMID: 24795464
  49. Downregulation of ICAM-1 mitigates MC38 colonic adenocarcinoma invasion. PMID: 24360118
  50. beta2 integrin-mediated neutrophil crawling on endothelial ICAM-1 and ICAM-2 is a prerequisite for transcellular neutrophil diapedesis across the inflamed BBB. PMID: 24259506

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Subcellular Location Membrane, Single-pass type I membrane protein
Protein Families Immunoglobulin superfamily, ICAM family
Tissue Specificity Expressed at low level on a subpopulation of lymphocytes, macrophages, and endothelial cells, but is strongly induced on these cells, and on fibroblasts and epithelial cells.
Database Links

KEGG: mmu:15894

STRING: 10090.ENSMUSP00000083587

UniGene: Mm.435508

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