Mouse parathyroid hormone (PTH) ELISA kit

Instructions
Code CSB-E08690m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Target Name parathyroid hormone (PTH)
Abbreviation PTH
Species Mus musculus (Mouse)
Sample Types serum, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 87-99    
1:2 Average % 100    
Range % 96-104    
1:4 Average % 90    
Range % 84-94    
1:8 Average % 96    
Range % 89-100    
Recovery
The recovery of mouse PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 90 84-95    
EDTA plasma (n=4) 104 97-110    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
400 2.670 2.584 2.627 2.507    
200 1.997 1.937 1.967 1.847    
100 1.360 1.339 1.350 1.230    
50 0.931 0.900 0.916 0.796    
25 0.514 0.501 0.508 0.388    
12.5 0.306 0.298 0.302 0.182    
6.25 0.224 0.221 0.223 0.103    
0 0.122 0.118 0.120      
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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