Mouse parathyroid hormone (PTH) ELISA kit

Code CSB-E08690m
Size 96T,5×96T,10×96T
Trial Size 24T ELISA kits trial application
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Product Details

Description

The product CSB-E08690m is a sandwich ELISA kit developed to measure levels of mouse parathyroid hormone (PTH) in serum or tissue homogenates. The enzyme-substrate chromogenic reaction is also used to amplify the signal and quantify the levels of the analyte through the intensity of the colored product. The color intensity positively correlates with the amount of PTH bound in the initial step.

PTH is a polypeptide synthesized and secreted by the parathyroid gland in response to low calcium levels detected in the blood. PTH promotes the production of active vitamin D and calcitriol (1,25-dihydroxycholecalciferol) in the kidneys. In conjunction with calcitriol, PTH regulates calcium and phosphate. PTH effects are present in the bones, kidneys, and small intestines. When serum calcium levels decrease, the secretion of PTH by the parathyroid gland elevates. Increased calcium levels in the serum serve as a negative-feedback loop signaling the parathyroid glands to stop the release of PTH.

Target Name parathyroid hormone (PTH)
Abbreviation PTH
Species Mus musculus (Mouse)
Sample Types serum, tissue homogenates
Detection Range 6.25 pg/mL-400 pg/mL
Sensitivity 1.56 pg/mL
Assay Time 1-5h
Sample Volume 50-100ul
Detection Wavelength 450 nm
Research Area Signal Transduction
Assay Principle quantitative
Measurement Sandwich
Precision
Intra-assay Precision (Precision within an assay): CV%<8%        
Three samples of known concentration were tested twenty times on one plate to assess.    
Inter-assay Precision (Precision between assays): CV%<10%        
Three samples of known concentration were tested in twenty assays to assess.      
               
Linearity
To assess the linearity of the assay, samples were spiked with high concentrations of mouse PTH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.  
  Sample Serum(n=4)    
1:1 Average % 94    
Range % 87-99    
1:2 Average % 100    
Range % 96-104    
1:4 Average % 90    
Range % 84-94    
1:8 Average % 96    
Range % 89-100    
Recovery
The recovery of mouse PTH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.  
 
Sample Type Average % Recovery Range    
Serum (n=5) 90 84-95    
EDTA plasma (n=4) 104 97-110    
               
               
Typical Data
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.  
 
pg/ml OD1 OD2 Average Corrected    
400 2.670 2.584 2.627 2.507    
200 1.997 1.937 1.967 1.847    
100 1.360 1.339 1.350 1.230    
50 0.931 0.900 0.916 0.796    
25 0.514 0.501 0.508 0.388    
12.5 0.306 0.298 0.302 0.182    
6.25 0.224 0.221 0.223 0.103    
0 0.122 0.118 0.120      
ELISA Data Analysis Watch ELISA data processing video & download Curve Expert if needed
Troubleshooting
and FAQs
ELISA kit FAQs
Storage Store at 2-8°C. Please refer to protocol.
Lead Time 3-5 working days

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